From Invasion to Biomaterial: Extraction and Comprehensive Characterization of Type I Collagen from the Invasive Gibel Carp (Carassius gibelio) Skins by Different Methods

Abstract

Driven by the escalating global demand for biopolymers, this study investigates the strategic potential of aquatic collagen as a superior alternative to conventional bovine and porcine sources. The research specifically focuses on extracting Type I collagen from the skin of Carassius gibelio (Prussian carp), an invasive species that threatens ecological stability in European and Turkish freshwater ecosystems. By evaluating three distinct extraction methodologies—Alcalase-assisted (A), pepsin-assisted (B), and phosphate-based (C)—the study analyzes their impact on yield, purity, and structural integrity. Characterization through pH analysis, SDS-PAGE, DSC, and SEM confirms that C. gibelio provides high-quality collagen suitable for biomedical and nutritional applications. Furthermore, the results demonstrate that extraction parameters can be tailored to modulate surface morphology and solubility.

Keywords

• invasive
• biomaterial
• valorisation
• characterisation

Supporting Institution

Eskişehir Osmangazi University

Project Number

FHD-2022-2514

Ethical Statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

İstiladan Biyomateryale: İstilacı Gümüşi Sazanı (Carassius gibelio) Derisinden Farklı Yöntemlerle Tip I Kolajen Ekstraksiyonu ve Karakterizasyonu

Abstract

Biyopolimerlere yönelik artan küresel talepten hareketle, bu çalışma, geleneksel sığır ve domuz kaynaklarına üstün bir alternatif olarak aquatic kolajenin stratejik potansiyelini incelemektedir. Araştırma, özellikle Avrupa ve Türkiye'deki tatlı su ekosistemlerinin ekolojik dengesini tehdit eden istilacı bir tür olan Carassius gibelio (Gümüşi sazan) derisinden Tip I kolajen ekstraksiyonuna odaklanmaktadır. Üç farklı ekstraksiyon yöntemini değerlendirerek—Alcalase destekli (A), pepsin destekli (B) ve fosfat bazlı (C) yöntemler—çalışma, bu yöntemlerin verim, saflık ve yapısal bütünlük üzerindeki etkilerini analiz etmektedir. pH analizi, SDS-PAGE, DSC ve SEM yoluyla yapılan karakterizasyon, C. gibelio’nun biyomedikal ve beslenme uygulamaları için uygun yüksek kaliteli kolajen sağladığını doğrulamaktadır.

Keywords

• İstilacı
• biyomateryal
• katma değer
• karakterizasyon

Supporting Institution

Eskişehir Osmangazi üniversitesi

Project Number

FHD-2022-2514

Ethical Statement

Yazarlar, bu makalede sunulan çalışmayı etkilemiş gibi görünebilecek herhangi bir bilinen çıkar çatışması veya kişisel ilişkinin bulunmadığını beyan ederler.

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