Assessment of the Cytotoxicity of Melia azedarach L. Extracts on Human Adipose-derived Mesenchymal Stem Cells

Abstract

I n this study, aqueous extracts of Melia azedarach L. green fruit and leaves were obtained using two different extraction methods. The extraction yields of the green fruit and leaves were found as 24.11% and 37.98% for the infusion method; 17.76% and 27.00% for the rotating method, respectively. The total phenolic content, related to the infusion method, was ascertained for green fruit extract 173.67±10.84 mg Gallic Acid Equivalent (GAE)/g dry weight and leaf extract 312.33±9.81 mg GAE/g dry weight. In other respects, antioxidant activity related to the infusion method was determined for green fruit extract 172.51±13.23 mg Trolox/L and leaf extract 569.16±10.41 mg Trolox/L. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to identify the chemical composition of the extracts. The cytotoxicity levels of the extracts were assessed on human adipose-derived mesenchymal stem cells (hAMSCs) using commercially available XTT assay. Consequently, it has been found that the green fruit extract has more cytotoxic activity than the leaf extract on hAMSCs.

Keywords

• Melia azedarach L.,toplam fenolik bileşikler,sitotoksisite,insan adipoz kaynaklı mezenkimal kök hücreler

References

1. S.S. Handa, S.P.S. Khanuja, G. Longo, D.D. Rakesh, Extraction technologies for medicinal and aromatic plants, J. Nat. Prod., 5 (2008) 440.
2. B. Biswas, K. Rogers, F. Mclaughlin, D. Daniels, A. Yadav, Antimicrobial activities of leaf extracts of guava (Psidium guajava l.) on two gram-negative and gram-positive bacteria, Int. J. Microbiol., (2013) 1-6.
3. M. Azam, A. Mamun-Or-Rashid, N. Towfique, M. Sen, S. Nasrin, Pharmacological potentials of Melia azedarach L., A review, Am. J. Biosci., 1 (2013) 44-49.
4. I. Khan, M. Yasinzai, Z. Mehmood, I. Ilahi, Comparative study of green fruit extract of Melia azedarach linn. with its ripe fruit extract for antileishmanial, larvicidal, antioxidant and cytotoxic activity, Asian J. Nat. Appl. Sci., 2 (2014) 442-454.
5. C. Carpinella, C. Ferrayoli, G. Valladares, M. Defago, S. Palacios, Potent limonoid insect antifeedant from Melia azedarach, Biosci. Biotechnol. Biochem., 66 (2002) 1731-1736.
6. T. Akihisa, X. Pan, Y. Nakamura, T. Kikuchi, N. Takahashi, M. Matsumoto, E. Ogihara, M. Fukatsu, K. Koike, H. Tokuda, Limonoids from the fruits of Melia azedarach and their cytotoxic activities, Phytochemistry, 89 (2013) 59-70.
7. K. Sangeetha, S. Rajarajan, In vitro antiviral activity of indian medicinal plants to asian and east central south african lineage of Chikungunya virus, Int. J. Pharm. Sci. Res., 6 (2015) 692-697.
8. B. Asokan, B. Rajkumar, S. Balamuruganvelu, S. Jaikumar, In vivo antidiabetic activity of ethanolic flower extract Melia azedarach L., Acta Biomed. Sci., 2 (2015) 215-217.

9. S. Sultana, H.M. Asif, N. Akhtar, M. Waqas, S.U. Rehman, Comprehensive review on ethanobotanical uses, phytochemistry and pharmacological properties of Melia azedarach linn., Asian J. Pharm. Res Heal. Care, 6 (2014) 26-32.
10. J. Yu, Y. Wang, Z. Deng, L. Tang, Y. Li, J. Shi, Y. Jin, Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells, Biol. Cell, 99 (2007) 465-474.
11. H. Jin, Y. Bae, M. Kim, S.-J. Kwon, H. Jeon, S. Choi, S. Kim, Y. Yang, W. Oh, J. Chang, Comparative analysis of human mesenchymal stem cells from bone marrow, adipose tissue, and umbilical cord blood as sources of cell therapy, Int. J. Mol. Sci., 14 (2013) 17986-18001.
12. Y.E. Arslan, M.M. Hız, T. Sezgin Arslan, The use of decellularized animal tissues in regenerative therapies, Kafkas Univ. Vet. Fak. Derg., 21 (2015) 139- 145.
13. I. Jun, Y. Bin Lee, Y.S. Choi, A.J. Engler, H. Park, H. Shin, Transfer stamping of human mesenchymal stem cell patches using thermally expandable hydrogels with tunable cell-adhesive properties, Biomaterials, 54 (2015) 44-54.
14. M.T. Sutton, D. Fletcher, S.K. Ghosh, A. Weinberg, R. van Heeckeren, S. Kaur, Z. Sadeghi, A. Hijaz, J. Reese, H.M. Jane, D.P. Lennon, A.I. Caplan, T.L. Bonfield, Antimicrobial properties of mesenchymal stem cells: therapeutic potential for cystic fibrosis infection, and treatment., Stem Cells Int., 2016 (2016) 1-12.
15. V.L. Singleton, J.A. Rossi, J. Jr, Colorimetry of total phenolics with acid reagents, Am. J. Enol. Vitic., 16 (1965) 144-158.
16. E. Erten, T. Arslan Sezgin, B. Derkus, Y.E. Arslan, Detergent-free decellularization of bovine costal cartilage for chondrogenic differentiation of human adipose mesenchymal stem cells in vitro, RSC Adv., 6 (2016) 94236-94246.
17. Y.E. Arslan, T. Sezgin Arslan, B. Derkus, E. Emregul, K.C. Emregul, Fabrication of human hair keratin/ jellyfish collagen/eggshell-derived hydroxyapatite osteoinductive biocomposite scaffolds for bone tissue engineering: From waste to regenerative medicine products, Coll. Surf. B Biointerf., 154 (2017) 160-170.
18. A.V. Khân, A.A. Khan, I. Shukla, In vitro antibacterial potential of Melia azedarach crude leaf extracts against some human pathogenic bacterial strains, Ethnobot. Leafl., 12 (2008) 439-445.
19. J. Kubola, S. Siriamornpun, Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro, Food Chem., 110 (2008) 881-890.
20. G. Zengin, C. Sarikurkcu, P. Uyar, A. Aktumsek, S. Uysal, M.S. Kocak, R. Ceylan, Crepis foetida L. subsp. rhoeadifolia (Bieb.) Celak. as a source of multifunctional agents: Cytotoxic and phytochemical evaluation, J. Funct. Foods, 17 (2015) 698-708.
21. R. Apak, K. Güçlü, M. Özyürek, S.E. Karademir, Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method, J. Agric. Food Chem., 52 (2004) 7970-7981.
22. S.D. Çekiç, K.S. Başkan, E. Tütem, R. Apak, Modified cupric reducing antioxidant capacity (CUPRAC) assay for measuring the antioxidant capacities of thiolcontaining proteins in admixture with polyphenols, Talanta, 79 (2009) 344-351.
23. H. Aoudia, B.D. Oomah, F. Zaidi, R. Zaidi-Yahiaoui, J.C.G. Drover, J.E. Harrison, Phenolics, antioxidant and anti-inflammatory activities of Melia azedarach extracts, Int. J. Appl. Res. Nat. Prod., 6 (2013) 19-29.
24. N.G. Ntalli, S. Vargiu, U. Menkissoglu-Spiroudi, P. Caboni, Nematicidal carboxylic acids and aldehydes from Melia azedarach fruits, J. Agric. Food Chem., 58 (2010) 11390-11394.
25. A. Sen, A. Batra, Chemical composition of methanol extract of the leaves of Melia azedarach L., Asian J. Pharm. Clin. Res., 5 (2012) 42–45.