A new, sensitive and disposable electrochemical immunosensor based on Benzaldehyde side group containing phosphazene polymer modified ITO substrate for Interleukin 1β detection

Abstract

Bu çalışmada, interlökin 1β (IL 1β) tespiti için tek kullanımlık benzaldehit ikameli fosfazen polimeri (BSPP) ile modifiye edilmiş ITO elektrot temelli yeni bir elektrokimyasal ultrahassas immünosensör geliştirilmiştir. Aldehit yan grupları içeren fosfazen polimeri (BSPP) halka açma polimerizasyonu yöntemi ile sentezlenmiştir. Bu aldehit grupları, anti-IL 1β antikorları için bir bağlantı noktaları sağlamıştır. Önerilen immünosensörün üretim süreci, Elektrokimyasal Empedans Spektroskopisi (EIS) ve Döngüsel Voltammetri (CV) gibi elektrokimyasal tekniklerle izlendi. Ek olarak, bu üretim aşamaları, Taramalı Elektron Mikroskobu (FE-SEM) ve Atomik Kuvvet Mikroskobu (AFM) kullanılarak karakterize edildi. Ayrıca, polimer kaplı elektrot yüzeyindeki BSPP polimer tabakası, Enerji Dağıtıcı X-ışını (EDX) kullanılarak araştırıldı. Üretilen immünosensör düşük bir tespit sınırına (9,3 fg / mL) ve geniş bir doğrusal tayin aralığına (0,03-7,5 pg / mL) sahiptir. Ayrıca, iyi tekrarlanabilirlik (% 1.82), mükemmel tekrarlanabilirlik (% 1.56), iyi seçicilik ve yüksek stabiliteye sahipti. Deney sonuçları, BSPP polimerinin, klinik teşhis ve pratik uygulamalarda IL1β antijen tespiti için arzu edilen bir platform olduğunu gösterdi. Önerilen biyosensörün uygulanabilirliği, insan serumunda IL 1β seviyesi ölçülerek test edildi ve önerilen immünosensör, nicel analiz için kabul edilebilir sonuçlara sahipti.

Keywords

• Poly(phosphazene),Label free immunosensor,Electrochemical impedance spectroscopy

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