The Effect of Aqueous Extract of Pine Kindling on the Liver and Kidney Tissues of Diabetic Rats

Abstract

This study aimed to investigate the effect of aqueous extracts from the obtained kindling wood of pine trees on liver and kidney tissues in diabetes induced by streptozotocin in rats. Male Sprague Dawley rats were divided into five groups: 1) Controls, 2) STZ rats 3) STZ rats treated with aqueous extracts from the obtained kindling wood of pine trees (different doses) for 10 weeks. According to our results, it was determined that these extracts show beneficial effects on oxidative stress in liver and kidney tissues. In vitro studies, bovine, porcine pancreas, fungi and bacteria derived from amylase on enzyme activity of the pine extracts haven’t been observed the inhibitory effect. Also disaccharides α-glycosidase enzyme that hydrolyzes on kindling of the pine was found to not have an inhibitory effect.

Keywords

• Amylase and glucosidas enzymes,Oxidative stress,Pine kindling wood

References

1. H. Ahn, G.W. Go, Pinus densiflora bark extract (PineXol) decreases adiposity in mice down regulation of hepatic de novo lipogenesis and adipogenesis in white adipose tissue, J. Microbiol. Biotechnol., 27(4) 2017 660-667.
2. T.P. Akerboom, H. Sies, Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples, Methods Enzymol., 77 1981 373-82. M. Aktaş, U. Değirmenci, S.K. Ercan, L. Tamer, U. Atik, Redükte glutatyon ölçümünde HPLC ve spektrofotometrik yöntemlerin karşılaştırılması, Türk Klinik Biyokimya Derg., 3(3) 2005 95-99.
3. F. Babaee, L. Safaeian, B. Zolfaghari, S. Haghjoo Javanmard, Cytoprotective effect of hydroalcoholic extract of Pinus eldarica bark against H2O2-induced oxidative stress in human endothelial cells, Iran Biomed., J., 20(3) 2016 161-7.
4. T. Balasubramanian, M. Karthikeyan, K.P. Muhammed Anees, C.P. Kadeeja, K. Jaseela, Antidiabetic and antioxidant potentials of Amaranthus hybridus in streptozotocin-induced diabetic rats, J. Diet Suppl., 14(4) 2017 395-410.
5. T. Baytop, Therapy with Medicinal Plants in Turkey (Past and Present), Istanbul University, Istanbul, Turkey, 2001.
6. R.R. Brenner, Hormonal modulation of delta6 and delta5 desaturases: case of diabetes, Prostaglandins Leukot Essent Fatty Acids, 68(2) 2003 151-62.W.W. Christie, Gas Chromatography and Lipids. The Oil Press, Glaskow, 1992.
7. S. Dewanjee, A.K. Das, R. Sahu, M. Gangopadhyay, Antidiabetic activity of Diospyros peregrina fruit: effect on hyperglycemia, hyperlipidemia and augmented oxidative stress in experimental type 2 diabetes, Food Chem. Toxicol., 47(10) 2009 2679-85.
8. GL. Ellman, Tissue sulfhydryl groups, Arch. Biochem. Biophys., 82(1) 1959 70-7.

9. N. Erdal, S. Gürgül, S. Kavak, A. Yildiz, M. Emre, Deterioration of bone quality by streptozotocin (STZ)-induced type 2 diabetes mellitus in rats, Biol. Trace. Elem. Res., 140(3) 2011 342-53.
10. A. Hara, N.S. Radin, Lipid extraction of tissues with a low-toxicity solvent, Anal. Biochem., 90(1) 1978 420-6.
11. W.J. Hasid, S. Abraham, Carbohydrates, S.P.Colowick, O.N, Kaplan (eds) Methods in Enzymology Chemical procedures for analysis of polysaccharides. Vol III, Academic Press, New York, 1957.
12. H.E. Joo, H.J. Lee, E.J. Sohn, M.H. Lee, H.S. Ko, S.J. Jeong, H.J. Lee, S.H. Kim, Anti-diabetic potential of the essential oil of Pinus koraiensis leaves toward streptozotocin-treated mice and HIT-T15 pancreatic β cells, Biosci. Biotechnol. Biochem., 77(10) 2013 1997-2001.
13. N.J. Kruger, A. Von Schaewen, The oxidative pentose phosphate pathway: structure and organization, Curr. Opin. Plant Biol., 6(3) 2003 236-46.
14. Y. Li, Y. Liu, G. Chen, Vitamin A status affects the plasma parameters and regulation of hepatic genes in streptozotocin-induced diabetic rats, Biochimie, 137 2017 1-11.
15. J. López-Cervantes, D.I. Sánchez-Machado, N.J. Ríos-Vázquez, High-performance liquid chromatography method for the simultaneous quantification of retinol, alpha-tocopherol, and cholesterol in shrimp waste hydrolysate, J. Chromatogr. A 1105(1-2) 2006 135-9.
16. O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 1951, 265-275.
17. T. Mašek, N. Filipović, L.F. Hamzić, L. Puljak, K. Starčević, Long-term streptozotocin diabetes impairs arachidonic and docosahexaenoic acid metabolism and Δ5 desaturation indices in aged rats, Experimental Gerontology 60 2014 140–146.
18. H. Miyazaki, K. Takitani, M. Koh, R. Takaya, A. Yoden, H. Tamai, α-Tocopherol status and expression of α-tocopherol transfer protein in type 2 diabetic Goto-Kakizaki rats, J. Nutr. Sci. Vitaminol. (Tokyo), 59(1) 2013 64-8.
19. A.G. Moat, J.W. Foster, M.P. Spector, Microbial Physiology, Central Pathways of Carbohydrate Metabolism. Wiley-Liss, Inc, New York, 2003.
20. U. Muruganathan, S. Srinivasan, V. Vinothkumar, Antidiabetogenic efficiency of menthol, improves glucose homeostasis and attenuates pancreatic β-cell apoptosis in streptozotocin-nicotinamide induced experimental rats through ameliorating glucose metabolic enzymes, Biomed. Pharmacother., 92 2017 229-239.
21. N. Mushtaq, R. Schmatz, M. Ahmed, L.B. Pereira, P. da Costa, K.P. Reichert, D. Dalenogare, L.P. Pelinson, J.M. Vieira, N. Stefanello, L.S. de Oliveira, N. Mulinacci, M. Bellumori, V.M. Morsch, M.R. Schetinger, Protective effect of rosmarinic acid against oxidative stress biomarkers in liver and kidney of strepotozotocin-induced diabetic rats, J. Physiol. Biochem., 71(4); 2015:743-51.
22. R. Naresh Kumar, R. Sundaram, P. Shanthi, Protective role of 20-OH ecdysone on lipid profile and tissue fatty acid changes in streptozotocin induced diabetic rats, Eur. J. Pharmacol., 698(1-3) 2013 489-98.
23. D.L. Nelson, M.M. Cox, Lehninger Biyokimyanın İlkeleri (Kılıç, N.), Palme Yayınları, Ankara, 2005.
24. H. Ohkawa, N. Ohishi, K. Yagi, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal. Biochem., 95(2) 1979 351-8.
25. K. Parveen, M.R. Khan, M. Mujeeb, W.A. Siddiqui, Protective effects of Pycnogenol on hyperglycemia-induced oxidative damage in the liver of type 2 diabetic rats, Chem. Biol. Interact., 186(2) 2010 219-27.
26. B. Ramesh, P. Viswanathan, K.V. Pugalendi, Protective effect of Umbelliferone on membranous fatty acid composition in streptozotocin-induced diabetic rats, Eur. J. Pharmacol., 566(1-3) 2007 231-9.
27. K.M. Ramkumar, R.S. Vijayakumar, P. Ponmanickam, S. Velayuthaprabhu, G. Archunan, P. Rajaguru Antihyperlipidaemic effect of Gymnema montanum: a study on lipid profile and fatty acid composition in experimental diabetes. Basic Clin. Pharmacol. Toxicol., 103(6) 2008 538-45.
28. S. Samarghandian, M. Azimi-Nezhad, T. Farkhondeh, Catechin treatment ameliorates diabetes and its complications in streptozotocin-induced diabetic rats, Dose Response, 15(1) 2017 1-7.
29. D.I. Sánchez-Machado, J. López-Hernández, P. Paseiro-Losada, High-performance liquid chromatographic determination of alpha-tocopherol in macroalgae, J. Chromatogr. A 976(1-2) 2002 277-84.
30. G. Saravanan, P. Ponmurugan, Ameliorative potential of S-allylcysteine: effect on lipid profile and changes in tissue fatty acid composition in experimental diabetes, Exp. Toxicol. Pathol., 64(6) 2012 639-44.
31. K. Takitani, K. Inoue, M. Koh, H. Miyazaki, K. Kishi, A. Inoue, H.Tamai, α-Tocopherol status and altered expression of α-tocopherol-related proteins in streptozotocin-induced type 1 diabetes in rat models. J. Nutr. Sci. Vitaminol. (Tokyo) 60(6) 2014 380-6.
32. P.J. Tuitoek, S.J. Ritter, J.E. Smith, T.K. Basu Streptozotocin-induced diabetes lowers retinol-binding protein and transthyretin concentrations in rats, Br. J. Nutr., 76(6) 1996 891-7.
33. E. Tuzlacı, M.K. Erol, Turkish folk medicinal plants, Part II: Eğridir (Isparta), Fitoterapia, 70 1999 593–610.
34. E. Tvrzická, M. Vecka, B. Staňková, A .Žák, Analysis of fatty acids in plasma lipoproteins by gas chromatography–flame ionization detection: Quantitative aspects. Analytica Chimica Acta 465 2002 337-350.
35. M. Wang, H.L. Ma, B. Liu, H.B. Wang, H. Xie, R.D. Li, J.F. Wang, Pinus massoniana bark extract protects against oxidative damage in L-02 hepatic cells and mice. Am. J. Chin Med., 38(5) 2010 909-19.
36. X.T. Wang, J. Li, L. Liu, N. Hu, S. Jin, C. Liu, D. Mei, X.D. Liu, Tissue cholesterol content alterations in streptozotocin-induced diabetic rats. Acta Pharmacol Sin., 33(7) 2012 909-17.
37. J. Yi, H. Qu, Y. Wu, Z. Wang, L. Wang, Study on antitumor, antioxidant and immunoregulatory activities of the purified polyphenols from pinecone of Pinus koraiensis on tumor-bearing S180 mice in vivo, Int. J Biol. Macromol., 94(PtA) 2017 735-744.