The Influence of the Myricetin on the Liver, Kidney, Spleen and Some Endocrine Glands of Male Rats at Prepubertal Period

Yıl 2019, Cilt: 47 Sayı: 3, 317 - 326, 23.10.2019

Nurhayat Barlas Aslı Okan Oflamaz

https://doi.org/10.15671/hjbc.510820

Öz

In this study, the effects of myricetin exposure to rats from postnatal day (PND) 23 to 53 at various doses were investigated. The male rats were divided into five groups and each group consisted of six animals. Group of rats were treated with myricetin 25 and 50 mg/kg/day in a suspension of corn oil. Positive control males were received gavage orally with 17α-ethinyl estradiol 0.7 and 7 µg /kg /body weight day and control males were received corn oil. End of the study, weights of liver, kidney, spleen, pancreas, thymus and adrenal gland were measured. Organ/body weight ratios were calculated and tissue sections were examined histologically. In liver, the TUNEL method was applied and evaluated. In results, absolute liver weights were decreased statistically in 0.7 and 7 µg/kg/day etinil estradiol and 50 mg/kg/day myricetin treatment groups, compared with the oil control group. Histopathological examination of the liver, kidney and spleen revealed significantly increased frequency of congestion, cell degeneration and mononuclear cell infiltration when compared with the control group. Also myricetin dose groups, the apoptotic cells were increased. This study demonstrated that orally gavages myricetin caused adverse effects on male liver, kidney, spleen and endocrine glands, during peripubertal period to pubertal period.

Anahtar Kelimeler

Phytoestrogen, myricetin, endocrine glands, prepubertal male rat

Kaynakça

• Referans 1 A. Okan, N. Barlas, G. Karabulut, Investigation of effects of myricetin on thyroid-gonadal axis of male rats at prepubertal period. Environ. Tox. Pharmacol., 40(2015) (1), 268-279.
• Referans 2. G.D. Castro, N.Q. Leanordo, M.E. Maciel, J.A. Castro, Preventive effects of plant polyphenols in the promotion of mammary cancer and testicular damage induced by alcohol drinking, Chapter 88, Polyphenols in Human Health and Dis. (2014) 1181-1190.
• Referans 3. A.V. Sirotkin, A.H. Harrath, Phytoestrogens and their effects. Europ. J. Pharmacol., 741 (2014) 230-236.
• Referans 4. J.A. Ross, C.M. Kasum, Dietary flavonoids: bioavailability, metabolic effects, and safety, Ann. Rev. Nutritio., 22 (2002) 19-34.
• Referans 5. F. Sun, X.Y. Zheng, J. Ye, T.T. Wu, J. Wang, W. Chen, Potential anticancer activity of myricetin in human T24 bladder cancer cells both in vitro and in vivo, Nutr. Cancer., 64 (2012) 599–606
• .Referans 6. S.K. Jung, K.W. Lee, S. Byun, N.J. Kang, S.H. Lim, Y.S. Heo, A.M. Bode, G.T. Bowden, H.J Lee, Z.Dong, Myricetin suppresses UVB-induced skin cancer by targeting Fyn, Cancer Res., 68 (2008) 6021–6029.
• Referans7. C.J. Weng, G.C. Yen, Flavonoids, a ubiquitous dietary phenolic subclass, exert extensive in vitro anti-invasive and in vivo anti-metastatic activities, Cancer Metas. Rev., 31 (2012) 323–351.
• Referans 8. S. Zhang, L. Wang, H. Liu, G. Zhao and L. Ming, Enhancement of recombinant myricetin on the radiosensitivity of lung cancer A549 and H1299 cell, Diagnos. Pathol., 9 (2014) 68.
• Referans 9. J.P.E. Spencer, Flavonoids: modulators of brain function? British J Nutrit., 99 (2008) E-Suppl. 1, ES60–ES77. Referans 10. R. Henneberg, M. Fleith, O. Aline, E.F. Furman, P. Hermann, A.J. Nascimento, M.SS. Leonart, Protective effect of flavonoids against reactive oxygen species production in sickle cell anemia patients treated with hydroxyurea, Revis. Brasil. Hematol. Hemoterapia., 35(1) (2013) 52-55.
• Referans 11. R. Bertin, Z. Chen, R. Marin, M. Donati, A. Feltrinelli, M. Montopoli, S. Zambon, E. Manzato, G. Froldi, Activity of myricetin and other plant-derived polyhydroxyl compounds in human LDL and human vascular endothelial cells against oxidative stress, Biomed. Pharmac., 82 (2016) 472-478.
• Referans 12. M. Mu, P. An, Q. Wu, X. Shen, D. Shao, H. Wang, Y. Zhang, S. Zhang, H. Yao, J. Min, F. Wang, The dietary flavonoid myricetin regulates iron homeostasis by suppressing hepcidin expression, The J. Nutrit. Biochem., 30 (2016) 53-61.
• Referans 13. H. Pan, Q. Hu, J. Wang, Z. Liu, D. Wu, W. Lu, J. Huang, Myricetin is a novel inhibitor of human inosine 5′-monophosphate dehydrogenase with anti-leukemia activity, Biochem. Biophys. Res. Commn., 477(4) (2016) 915-922.
• Referans 14. O. Ganry, Phytoestrogens and prostate cancer risk. Preven. Medic. 41(1) (2005) 1-6.
• Referans 15. D.K. Semwal, R.B. Semwal, S. Combrinck, A. Viljoen, Myricetin: A dietary molecule with diverse biological activities, Nutrients., 8(2) (2016) 1-31.
• Referans 16. N. Barlas, S. Özer, G. Karabulut, The estrogenic effects of apigenin, phloretin and myricetin based on uterotrophic assay in immature Wistar albino rats, Toxic. Let., 226(1) (2014) 35-42.
• Referans 17. United States Environmental Protection Agency, Endocrine Disruptor Screening Program Test Guidelines 1500: Pubertal Development and Thyroid Function in Intact Juvenile/ Peripubertal Male Rats. OPPTS 890. (2009).
• Referans 18. H. Sugimoto, K. Shikata, M. Matsuda, M. Kushiro, Y. Hayashi, K. Hiragushi, J. Wada, H. Makino, Increased expression of endothelial cell nitric oxide synthase (ecnos) in afferent and glomerular endothelial cells is involved in glomerular hyperfiltration of diabetic nephropathy, Diabetologia., 41 (1998) 1426–1434.
• Referans 19. P.H.M. Peeters, L. Keinan-Boker, Y.T. van der Schouw, D.E. Grobbee, Phytoestrogens and breast cancer risk, Breast Cancer Res. Treat., 77 (2003) 171-183.
• Referans 20. M.S. Kurzer, X. Xu, Dietary Phytoestrogens, An. Rev. Nut. 17 (1997) 353-381.
• Referans 21. D.L. Trent, D.L. Edwin, Dietary soy phytoestrogens produce anxiolytic effects in the elevated plus-maze, Brain Research., 913 (2001) 180-184.
• Referans 22. R.C. Santhell, Y.C. Chang, M.G. Nair, W.G. Helferich, Dietary genistein exerts estrogenic effects upon the uterus, mammary, gland and the hypothalamic/pituitary axis in rats, The J. Nutrit., 127 (2) (1997) 263-269.
• Referans 23. J.W. Erdman, D. Balentine, L. Arab, G. Beecher, J.T. Dwyer, J. Folts, J. Harnly, P. Hollman, C.L. Keen, G. Mazza, M. Messina, A. Scalbert, J. Vita, G. Williamson, J. Burrowes, Flavonoids and heart health: Proceedings of the ILSI North America Flavonoids Workshop, The J. Nutrit., 137 (2007) 718-737.
• Referans 24. J. Wu, X. Wang, H. Chiba, M. Higuchi, T. Nakatani, O. Ezaki, H. Cui, K. Yamada, Y. Ishimi, Combined intervention of soy isoflavone and moderate exercise prevents body fat elevation and bone loss in ovariectomized mice, Metabol., 53(7) (2004) 942–948.
• Referans 25. K.C. Ong, H.E. Khoo, Biological effects of myricetin. General Pharmacology, The Vascular System., 29 (2) (1997) 121-126.
• Referans 26. S. Assinder, R. Davis, M. Fenwick, A. Glover, Adult-only exposure of male rats to a diet of high phytoestrogen content increases apoptosis of meiotic and post- meiotic germ cells. Reprod., 133 (1) (2007) 11-19.
• Referans 27. P.A. Phillips, V. Sangwan , B. D. orja-Cacho , V. Dudeja , S.M. Vickers, A.K. Saluja , Myricetin induces pancreatic cancer cell death via the induction of apoptosis and inhibition of the phosphatidylinositol 3-kinase (PI3K) signaling pathway. Cancer Let., 308 (2) (2011) 181-188.
• Referans 28. C.R. Cederroth, M. Vinciguerra, A. Gjinovci, F. Kuhne , M. Klein, M. Cederroth , D. Caille, M. Suter, D. Neumann, R.W. James, D.R. Doerge, T. Wallimann, P. Meda, M. Foti, F. Rohner-Jeanrenaud , J.D. Vassalli, S. Nef, Dietary phytoestrogens activate AMP-activated protein kinase with improvement in lipid and glucose metabolism, Diabetes., 57 (5) (2008) 1176–1185.
• Referans 29. G.E. Kelly, G.E. Joannou, A.Y. Reeder, C. Nelson, M.A. Waring, The variable metabolic response to dietary isoflavones in humans, Proceedings of the Society for Exper. Biol. Med., 208 (1) (1995) 40–43.