Biosynthesis of Gold Nanoparticles using Scytosiphon lomentaria (Brown algae) and Spyridia filamentosa (Red algae) from Kyrenia Region and Evaluation of their Antimicrobial and Antioxidant Activity

Year 2019, Volume: 47 Issue: 4, 367 - 382, 01.12.2019

Doga Kavaz , Huzaifa Umar Tariro Zimuto

https://doi.org/10.15671/hjbc.518593

Cited By: 11

Abstract

This
study was carried out for biosynthesis of gold nanoparticles using Scytosiphon lomentaria (brown algae) and Spyridia
filamentosa (red algae) and compared. Synthesized gold nanoparticles were
characterized using the UV-Vis spectroscopy (UV-Vis), Fourier transform
infrared (FTIR) and Master Sizer analysis. Antioxidant and antimicrobial
activity of the synthesized nanoparticles were tested against E. coli and S. typhi. Macro algae involvement in the stabilization of the gold
nanoparticles was confirmed by the presence of UV-Vis peak at 540 nm and is an
indication of the presence of the gold nanoparticles (AuNPs). Stretching in
peaks of the FTIR showed that the biomolecules present in the seaweed extract
reduced the gold ions. Master sizer results for AuNPs were within the range of
15-55 nm. Antioxidant activity carried out using DPPH free radical scavenging
activity revealed significant activity for both AuNPs. Biosynthesized AuNPs
also showed antimicrobial activity against Salmonella
typhii and Escherichia coli. The S. lomentaria gold nanoparticles
exhibited great inhibition against Escherichia
coli, whereas S. filamentosa gold
nanoparticles showed great antibacterial activity against S .typhi. Synthesized AuNPs using S. lomentaria and S.
filamentosa   as stabilizing agents showed convincing
antioxidant and antimicrobial activity against gram negative and gram positive
bacteria.

Keywords

Gold nanoparticles, antimicrobial activity, Spyridia filamentosa, Scytosiphon lomentaria

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