Preparation and Characterization of Nanoparticular Systems For Topical Treatment of Ocular Infections

Yıl 2020, Cilt: 48 Sayı: 1, 49 - 58, 17.04.2020

Ebru Erdal , Yeşim Aslan Altay , Nagihan Ugurlu

https://doi.org/10.15671/hjbc.552231

Cited By: 2

Öz

Çalışmada, PLGA nanopartiküllere besifloksasin etken maddesi yüklenmiş ve elde edilen yapının bakteriyel konjuktivit tedavisi için kontrollü ilaç salım sistemi olarak kullanılabilirliği araştırılmıştır. Besifloksasin, sistemik bir formülasyon içermediği ve hem gram-pozitif hem de gram-negative patojenlere karşı etkili olduğu için seçilmiştir. Besifoksasinin topikal uygulama sonrasında, uygulama bölgesindeki kalım süresini uzatmak amacıyla nanopartiküler formülasyonu hazırlanmış ve etkinliği test edilmiştir. Bunun için, bu çalışmada besifloksasin PLGA nanopartiküllere yüklenmiş, karakterize edilmiş ve in vitro salım çalışmaları yapılmıştır. Ayrıca besifloksasin yüklü PLGA nanopartiküllerin toksisitesi belirlenmiştir. Nanopartiküller, morfolojik yapısı taramalı elektron mikroskobu, boyut ve yük analizi Zeta Sizer, fizikokimyasal yapısı Fourier Dönüşümü Kızılötesi Spektrometresi (FTIR) ile karakterize edilmiştir. Zeta Sizer sonuçlarına göre nanopartiküllerin ortalama 200nm boyutuna sahip olduğu belirlenmiştir ve SEM görüntüleri de sonuçları destekler niteliktedir. Nanopartiküllerden besifloksasinin salım patterninin, burst effect şeklinde olmayıp daha yavaş ve kontrollü bir şekilde salındığı görülmüştür.

Anahtar Kelimeler

PLGA nanoparticles, besifloxacin, drug delivery systems, eye infection

Kaynakça

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