Antimicrobial Activity of Hemolymph and Venom Obtained from Some Scorpion Species

Yıl 2020, Cilt: 48 Sayı: 4, 349 - 354, 06.07.2020

Halil Koç Cumhur Avşar Yusuf Bayrakcı

https://doi.org/10.15671/hjbc.646190

Öz

The aim of this study
was to determine the antibacterial and antifungal activities of hemolymph and
venom obtained from some scorpion species. Venom samples were obtained by
electrical stimulation method from five different scorpion species. Again,
hemolymph fluids were obtained from five different scorpion species.
Antimicrobial activities of hemolymph and venom diluted 1:1 with sterile saline
were tested against a total of 10 microorganisms including seven bacteria and
three yeasts using disc diffusion method. Venom samples of Protoiurus kraepelini showed inhibition zones of 9-20 mm on all
microorganisms tested. Venom from this species has the highest antibacterial
activity against Bacillus subtilis. The venom samples of Mesobuthus gibbosus were effective (7-12
mm) on all microorganisms except Enterococcus faecalis and Micrococcus
luteus. In addition, it was found that venoms obtained from Androctonus crassicauda, Hottentotta saulcyi and Leiurus abdullahbayrami have different
levels of antibacterial effect but no antifungal effect against Candida
species. Scorpion hemolymphs have been shown to have similar effects against
Gram-positive and Gram-negative bacteria. Besides, the obtained hemolymphs have
been found to have low effect (7-9 mm) on microorganisms.

Anahtar Kelimeler

Scorpion, antimicrobial activity, venom

Teşekkür

We wish to thank Dr. Ersen Aydın Yağmur for his help during the field trips and specimen collecting. Special thanks to Prof. Dr. İsmet BERBER (Late).

Bazı Akrep Türlerinden Elde Edilen Hemolenf ve Zehrin Antimikrobiyal Aktivitesi

Öz

Bu çalışmanın amacı, bazı akrep türlerinden elde edilen hemolenf ve zehirlerin antibakteriyal ve antifungal özelliklerinin belirlenmesidir. Zehir örnekleri elektrik stimülasyonu yöntemiyle beş farklı akrep türünden elde edilmiştir. Bununla birlikte hemolenf sıvısı da beş farklı akrep türünden elde edilmiştir. 1:1 oranında tuz solüsyonu ile seyreltilen hemolenf ve zehirlerin antimikrobiyal aktiviteleri yedi bakteri ve üç mayadan oluşan 10 mikroorganizmaya karşı disk difüzyon yöntemi ile test edilmiştir. Protoiurus kraepelini’den elde edilen zehir örnekleri test edilen bütün mikroorganizmalar üzerinde 9-20 mm inhibisyon zonu meydana getirmiştir. Bu türden elde edilen zehir Bacillus subtilis’e karşı en yüksek antibakteriyal aktivite gösteren örnek olmuştur. Mesobuthus gibbosus’dan alınan zehir örnekleri Enterococcus faecalis ve Micrococcus luteus haricindeki tüm mikroorganizmalarda etkili (7-12 mm) olmuştur. Ayrıca, Androctonus crassicauda, Hottentotta saulcyi ve Leiurus abdullahbayrami’den elde edilen zehirler farklı düzeylerde antibakteriyal etkiye sahip olmakla birlikte Candida türleri üzerinde antifungal bir etkisi olmamıştır. Akrep hemolenflerinin Gram-pozitif ve Gram-negatif bakteriler üzerinde benzer etkilerinin olduğu gösterilmiştir. Bunun yanında, elde edilen hemolenflerin mikroorganizmalar üzerinde düşük etkiye (7-9 mm) sahip olduğu tespit edilmiştir

Anahtar Kelimeler

Akrep, antimikrobiyal aktivite, zehir, hemolenf

Kaynakça

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