Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography

Yıl 2007, Cilt: 35 Sayı: 2, 129 - 134, 01.04.2007

Nurullah Şanlı Senem Şanlı Güleren Ozkan

Öz

In this study, the determination of aromatic biogen amines; histamine, β-phenyl ethylamine, tyramine and tryptamine,and their precursor amino acids; histidine, phenylalanine, tyrosine and tyriptophan was performed without using anyderivatization reagents. Mobile phase was optimised by establishing relationships between retention parameters andReichardt’s ETNscale of solvent polarity. In addition, selectivity α and resolution RS values were used in order toestablish a general model relating elution behaviour of substances with the composition of mobile phase. In order tofind the optimum pH value, pKa values of amino acids in the mobile phase were determined by potentiometric methodin 20% and 30% v/v MeCN media. For biogenic amines and amino acids studied, the optimised mobile phase wascomposed of acetonitrile-water and 6.10-3mol l-11-heptanesulphonic acid sodium salt adjusted at pH 2.45 with a percentage of MeCN-water of 28% v/v

Anahtar Kelimeler

Biogenic amines, LC, Optimisation of mobile phase, Reichardt’s ETN scale of solvent polarity

Kaynakça

• 1. Shalaby, A.R., Significance of biogenic amines to food safety and human health, Food Res. Int., 29, 675-690, 1996.
• 2. Armağan, Ö., A review: Current analytical methods for the determination of biogenic amines in foods, Food Chemistry, 103, 1475–1486, 2007.
• 3. Edwards, R.A., Dainty, R.H., Hibbard, C.M., Ramantanis, S.V., Amines in fresh beef of normal pH and the role of bacteria in changes in concentration observed during storage in vacuum packs at chill temperatures. J. Appl. Bacteriol., 63, 427-434, 1987.
• 4. Lange, J., Thomas, K., & Wittmann, C., Comparison of a capillary electrophoresis method with high-performance liquid chromatography for the determination of biogenic amines in various food samples. J. Chromatogr. B., 779, 229–239, 2002.
• 5. Innocente, N., Biasutti, M., Padovese, M., Moret, S., Determination of biogenic amines in cheese using HPLC technique and direct derivatization of acid extract, Food Chemistry, 101, 1285–1289, 2007.
• 6. Loukou, Z., and Zotou, A., A Comparative Survey of the Simultaneous Ultraviolet and Fluorescence Detection in the RP-HPLC Determination of Dansylated Biogenic Amines in Alcoholic Beverages, Chromatographia, 58, 579-585, 2003.
• 7. Vidal-Carou, C., Lahoz-Portolés, F., Bover-Cid S., and Mariné-Font A., Ion-pair high-performance liquid chromatographic determination of biogenic amines and polyamines in wine and other alcoholic beverages, J. Chromatogr. A. 998, 235-241, 2003.
• 8. Lozanov, V., Petrov, S., and Mitev, V., Simultaneous analysis of amino acid and biogenic polyamines by high-performance liquid chromatography after precolumn derivatization with N-(9- fluorenylmethoxycarbonyloxy) succinimid, J.Chromatogr. A. 1025, 201-208, 2004.
• 9. Levin, S., and Grushka, E., Reversed-phase liquid chromatographic separation of amino acids with aqueous mobile phases containing copper ions and alkylsulfonates, Anal. Chem., 57, 1830-1835, 1985.
• 10. Walker, T.A., Pietrzyk, D.J., Ion-interaction chromatographic separation of free amino acids, J. Liquid Chromatogr.10 (1), 161-174, 1987.
• 11. Hajos, G., Sass-Kiss, A., Szerdahelyı, E., Bardocz, S., Changes in biogenic amine content of Tokaj Grapes, wines and Aszu wines, J. Food Sci., 65 (7), 1142-1144, 2000.
• 12. Reichardt, C., Solvent and Solvent Effects in Organic Chemistry, VCH, Weinheim. 1988.
• 13. Rondinini, S., Mussini, P.R., Mussini, T., References value standard and primary standard for pH measurements in organic solvents and water-organic solvents mixtures of moderate to high permittivities, Pure Appl. Chem., 59, 1549-1560, 1987.
• 14. Mussini, P.R., Mussini, T., Rondinini, S., Reference value standards for pH measurements in D2O and aqueous–organic solvent mixtures: New accessions and assessments, Pure Appl. Chem.69, 1007-1014, 1997.
• 15. Barbosa, J., Sanz-Nebot, V., Preferential solvation in acetonitrile-water mixtures. Relationship between solvatochromic parameters and standard pH values. J. Chem. Soc. Faraday Trans., 90, 3287-3292, 1994.
• 16. Barbosa, J., Marqués, I., Barrón, D., Sanz-Nebot, V., The application of factor analysis to solvatochromic parameters and pHs values for the standardization of potentiometric sensors in mobile phases used in liquid chromatography, TRAC Trends Anal. Chem., 18, 543-549, 1999.
• 17. Gran G., Determination of the equivalence point in potentiometric titration. Part II. Analyst, 77, 661-665, 1952.
• 18. Gameiro, P., Reis, S., Lima, J.L.F.C., De, Castro B., Calibration of pH glass electrodes by direct strong acid/strong base titrations under dilute conditions, Anal. Chim. Acta, 405, 167-172, 2000.
• 19. Barbosa, J., Barron, D., Beltran, J.L., Sanz-Nebot, V., PKPOT A program for the potentiometric study of ionic equilibria in aqueous and non-aqueous media, Anal. Chim. Acta.317, 75-81, 1995.
• 20. Marcus, Y., and Migron, Y., Polarity, hydrogen bonding, and structure of mixtures of water and cyanomethane. J. Phys. Chem., 95, 400-406, 1991.