Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification

Yıl 2008, Cilt: 36 Sayı: 1, 61 - 76, 01.01.2008

İlker Koç Nilay Bereli Gözde Baydemir Igor Yu Galaev Adil Denizli

Öz

Poly acrylamide-methacrylamidohistidine [poly AAm-MAH ] cryogel was prepared by bulk polymerization whichproceeds in aqueous solution of monomers frozen inside a syringe column cryo-polymerization . After thawing, themonolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 µm size. Poly AAmMAH cryogel was characterized by swelling studies, FTIR and scanning electron microscopy. The equilibrium swellingdegree of the poly AAm-MAH monolithic cryogel was 6.97 g H2O/g cryogel. Poly AAm-MAH cryogel was used in theadsorption/desorption of immunoglobulin G IgG from aqueous solutions and human plasma. The non-specificadsorption of IgG was very low 0.22 mg/g . The maximum amount of IgG adsorption from aqueous solution in HEPESbuffer was 32.879 mg/g at pH 7.4. Higher IgG adsorption value was obtained from human plasma up to 100 mg/g .Desorption of IgG from poly AAm-MAH cryogel was obtained using 20 mM Acetate buffer containing 1.0 M NaCl atpH 4. It was observed that IgG could be repeatedly adsorbed and desorbed with poly AAm-MAH cryogel withoutsignificant loss in the adsorption capacity

Anahtar Kelimeler

Affinity chromatography, cryogel, Protein Purification, IgG

Kaynakça

• 1. V.I. Lozinsky, F.M. Plieva, I.Y. Galaev, B. Mattiasson, Bioseparation 10 (2002) 163.
• 2. V.I. Lozinsky, I.Y. Galaev, F.M. Plieva, I.N. Savina, H. Jungvid, B. Mattiasson, TRENDS in Biotechnol., 21 (2003) 445.
• 3. P. Arvidsson, F.M. Plieva, V.I. Lozinsky, I.Y. Galaev, B. Mattiasson, J. Chromatogr. A 986 (2003) 275.
• 4. P. Arvidsson, F.M. Plieva, I.N. Savina, V.I. Lozinsky, S. Fexby, L. Bulow, I.Y. Galaev, B. Mattiasson, J. Chromatogr. A, 977 (2002) 27.
• 5. F. Svec, Electrophoresis, 27 (2006) 947.
• 6. L. Uzun, R. Say, A. Denizli, React. Funct. Polym. 64 (2005) 93.
• 7. C. Babac, H. Yavuz, I.Y. Galaev, E. Pişkin, A. Denizli, React. & Functl. Polym. 66 (2006) 163.
• 8. Guerrier, L., Girot, P., Schwartz, W., Boschetti, E., Bioseparation, 9 (2000) 211.
• 9. G. Tishchenko, J. Dybal, K. Meszarosova, Z. Sedlakova, M. Bleha, J. Chromatogr. A, 954 (2002) 115.
• 10. V.I. Muronetz, T. Korpela, J. Chromatogr. B, 790 (2003) 53.
• 11. T. Burnouf, M. Radosevich, J. Biochem. Biophys. Methods, 49 (2001) 575.
• 12. Tejeda-Mansir, R. Espinoza, R.M. Montesinos, R. Guzman, Bioproc. Eng., 17 (1997) 39.
• 13. A. Denizli, J.Chromatogr.B, 668 (1995) 13.
• 14. P. Füglistaller, J. Immunol. Methods 124 (1989) 171.
• 15. Denizli, Y. Arica, J. Biomater. Sci. Polym. Ed. 11 (2000) 367.
• 16. O. Pitiot, C. Legallais, L. Darnige, M.A. Vijayalakshmi, J. Membr. Sci. 166 (2000) 221.
• 17. O. Pitiot, L. Folley, M.A. Vijayalakshmi, J. Chromatogr. B, 758 (2001) 163.
• 18. Huang, P.Y., and Carbonell, R.G., Biotechnol. Bioeng., 63 (1999) 633-641.
• 19. M.A. Vijayalakshmi, Trends Biotechnol. 7 (1989) 71.
• 20. K. Haupt, M.A. Vijayalakshmi, J. Chromatogr. 644 (1993) 289.
• 21. C.I. Alvarez, M.C. Strumia, H.E. Bertorello, React. Functl. Polym., 34 (1997) 103.
• 22. A. Legallais, F.B. Anspach, S.M.A. Bueno, K. Haupt, M.A. Vijayalakshmi, J. Chromatogr. B, 691 (1997) 33.
• 23. K. Haupt, S.M.A. Bueno, M.A. Vijayalakshmi, J. Chromatogr. B, 674 (1995) 13.
• 24. A. El-Kak, S. Manjini, M.A. Vijayalakshmi, J. Chromatogr. B, 604 (1992) 29.
• 25. S.M.A. Bueno, K. Haupt, M.A. Vijayalakshmi, J. Chromatogr. B, 66 (1995) 57.
• 26. E. Klein, E. Eichholtz, D.H. Yeager, J. Membrane Sci., 95 (1994) 199.
• 27. M. Kim, K. Saito, S. Furusaki, S., Sato, T., Sugo, T., Ishigaki, I., 1991, J. Chromatogr., 585, 45.
• 28. D. Müller-Schulte, S. Manjini, M.A. Vijayalakshmi, J. Chromatogr., 539 (1991) 307.
• 29. A. Denizli and E. Pişkin, J. Chromatogr. B, 666 (1995) 215-222.
• 30. P. Langlotz, K.H. Kroner, J. Chromatogr., 591 (1992) 107.
• 31. O.P. Dancette, J.L. Taboureau, E. Tournier, C. Charcosset, P. Blond, J. Chromatogr. B., 723 (1999) 61.
• 32. S.F. Teng, K. Sproule, A. Husain, C.R. Lowe, J. Chromatogr. B., 740 (2000) 1.
• 33. S. Özkara, H. Yavuz, S. Patir, Y. Arica, A. Denizli, Sep. Sci. Technol., 37 (2002) 717.
• 34. S.F. Teng, K. Sproule, A. Hussain, C.R. Lowe, J. Mol. Recog., 12 (1999) 67.
• 35. G. Fassina, A. Verdoliva, G. Palombo, M. Ruvo, G. Cassani, J. Mol. Recog. 1998, 11, 128.