A Validated RP-HPLC Method for the Estimation of Ornidazole in Pharmaceutical Dosage Forms

Yıl 2012, Cilt: 40 Sayı: 4, 401 - 407, 01.09.2012

Senem Şanlı Tuğrul Yıldırım Bediha Akmeşe Nurullah Şanlı

Öz

Anew, simple, rapid, selective, precise and accurate isocratic reverse phase high performance liquid chromatography assay has been developed and validated for assay of Ornidazole in tablet formulations. Tinidazole was used as an internal standard. These compounds are separated well on a X-Terra Waters Corp., Milford, MA, USA RP-18 column by using a mobile phase consisting of a mixture of acetonitrile : water 30:70; v/v adjusted to pH 3.00 with 10 mM o-phosphoric acid at a flow rate of 1.0 mL min-1. The method was validated as per the ICH guidelines.

Anahtar Kelimeler

Ornidazole, RP-LC, Validation, Pharmaceutical Dosage Forms

Ornidazol’ün Farmasötik Dozaj Formlarından Valide Edilmiş RP-HPLC Metodu ile Belirlenmesi

Öz

O rnidazol için yeni, kolay, seçici, kesin ve doğru, izokratik ters faz sıvı kromatografik metot geliştirilmiş ve tablet formülasyonlarından miktar tayini için valide edilmiştir. Tinidazol iç standart olarak kullanılmıştır. Bu bileşikler için ayırma, X-Terra Waters Corp., Milford, MA, USA RP-18 kolonu ve mobil faz olarak 10 mM o-fosforik asit içeren pH 3’e ayarlanmış 30:70 v/v asetonitril-su karışımı kullanılarak 1.0 mL dakika-1 akış hızında gerçekleştirilmiştir. Metot ICH kurallarına göre valide edilmiştir

Anahtar Kelimeler

Ornidazol, RP-LC, Validasyon, Farmasötik Doz Formları

Kaynakça

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