Comparison of Six Commercial DNA Extraction Kits for DNA Extraction from Wheat

Yıl 2014, Cilt: 42 Sayı: 3, 395 - 400, 01.09.2014

Çağatay Karaaslan Hayriye Akel Sibel Ünlü Işık Perçin

Öz

In this study, six commercial DNA extraction kits were compared with each other in terms of DNA yield, DNA purity, amplification efficiency, the time spent and the cost per extraction. Extracted DNA samples were controlled by agarose gel electrophoresis and spectrophotometric measurement. The DNA samples were used as a template for 18SrRNA and chloroplast trnL–trnF intergenic spacer region amplifications to evaluate the PCR efficiency of DNA samples. Results showed that SIGMA GenEluteTM Plant Genomic DNA Miniprep Kit and QIAGEN DNeasy Plant Mini Kit are the most efficient commercial kits for DNA isolation from both wheat seed and leaf samples.

Anahtar Kelimeler

Wheat, DNA extraction kits, DNA isolation, Plant DNA

Buğdaydan DNA Ekstraksiyonu için Altı Ticari DNA Ekstraksiyon Kitinin Karşılaştırılması

Öz

B u çalışmada, 6 adet DNA ekstraksiyon kiti DNA verimi, DNA saflığı, amplifikasyon etkinliği, harcanan zaman ve ekstraksiyon başına düşen fiyat açısından karşılaştırılmıştır. Ekstrakte edilen DNA örnekleri, agaroz jel elektroforezi ve spektrofotometrik ölçümler ile kontrol edilmiştir. DNA örneklerinin PCR etkinliğini değerlendirmek için, ekstrakte edilen DNA örnekleri 18SrRNA kloroplast ve trnL–trnF genler arasındaki bölgenin amplifikasyonları için kalıp olarak kullanılmıştır. Sonuçlar, SIGMA GenElute™ Plant Genomic DNA Miniprep ve QIAGEN DNeasy Plant Mini kitlerinin buğday tohumundan ve yapraklarından DNA izolasyonunda en etkili ticari kitler olduğunu göstermiştir

Anahtar Kelimeler

Buğday, DNA ekstraksiyon kitleri, DNA izolasyonu, Bitki DNA’sı

Kaynakça

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