Purification of Lysozyme with Congo Red Immobilized Cryogel Discs

Year 2014, Volume: 42 Issue: 4, 563 - 568, 01.11.2014

Işık Perçin

Abstract

In this study, congo red immobilized poly hydroxyethyl methacrylate [CR-PHEMA] cryogel discs were pre- pared for purification of lysozyme. CR-PHEMA cryogel discs were characterized by swelling test, elemental analysis and scanning electron microscopy. Lysozyme adsorption was performed from aqueous solutions and maximum lysozyme adsorption capacity of CR-PHEMA cryogel discs was found to be 136.6 mg/g. Effects of initial lysozyme concentration, pH and ionic strength on lysozyme adsorption capacity of CR-PHEMA cryogel discs were investigated. Maximum adsorption was obtained at pH 8.0. In the last step of this study, lysozyme was purified from egg white using CR-PHEMA cryogel discs. Egg white fractions were observed on sodium do- decyl sulfate polyacrylamide gel electrophoresis.

Keywords

Congo red, lysozyme, PHEMA, cryogel

Kongo Kırmızısı İmmobilize Kriyojel Disklerle Lizozim Saflaştırılması

Abstract

B u çalışmada, lizozim saflaştırılması için congo kırmızısı immobilize edilmiş poli hidroksietil metakrilat [CRPHEMA] kriyojel diskler hazırlanmıştır. CR-PHEMA kriyojel diskler şişme testi, elementel analiz ve taramalı elektron mikroskobu ile karakterize edilmiştir. Lizozim adsorpsiyonu sulu çözeltilerden gerçekleştirilmiş ve CRPHEMA kriyojel disklerin maksimum lizozim adsorpsiyon kapasitesi 136.6 mg/g olarak bulunmuştur. Başlangıç lizozim derişiminin, pH’ın ve iyonik şiddetin CR-PHEMA kriyojel disklerin lizozim adsorpsiyon kapasitesine olan etkileri araştırılmıştır. Maksimum adsorpsiyon pH 8.0’de elde edilmiştir. Bu çalışmanın son basamağında, lizozim CR-PHEMA kriyojel diskler kullanılarak yumurta beyazından saflaştırılmıştır. Yumurta beyazı fraksiyonları sodyum dodesil sülfat poliakrilamid jel elektroforezinde incelenmiştir

Keywords

Congo kırmızısı, lizozim, PHEMA, kriyojel

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