Determination of Resistance in Pseudomonas aeruginosa Strains Against Beta-Lactam, Aminoglycoside and Ciprofloxacin Group Antibiotics

Year 2015, Volume: 43 Issue: 1, 59 - 67, 01.03.2015

Sezen Bilen Özyürek Işıl Seyis Bilkay

Abstract

Nowadays, due to widespread use of broad range spectrum antibiotics in treatments, Pseudomonas aerugi- nosa developed high levels of resistance to these antibiotics. Therefore it is challenging to treat the infec- tions caused by P. aeruginosa. In this study, multi-drug resistant strains and antibiotype groups were identified by investigating resistance in P. aeruginosa strains isolated from three different hospitals in Ankara, against beta-lactam, aminoglycoside and ciprofloxacin. Thus, it was aimed that the most effective antibiotics for treat- ment of P. aeruginosa sourced infections were determined. Sixty-nine P. aeruginosa strains were investigated for resistance against aztreonam, meropenem, imipenem, amikacin, tobramycin, piperacillin, ceftazidime, ce- fepime and ciprofloxacin. It was found that the highest resistance rate was against aztreonam 66.6% and the most effective antibiotic was found to be amikacin 5.7% . Additionally, the rate of multi-drug resistance in P. aeruginosa strains was determined as 36%. Although, 18 antibiotype groups were identified in P. aeruginosa strains, the highest antibiotype rate was observed in antibiotype 8 which was intermediate to aztronem and sensitive to other 8 antibiotics. In this respect, the combined treatment of aminoglycoside and ciprofloxacin is thought to be effective against resistant P.aeruginosa strains.

Keywords

Pseudomonas aeruginosa, Antibiotic resistance, Antibiotype, Multi-drug resistance

Pseudomonas aeruginosa Suşlarında Beta-Laktam, Aminoglikozid ve Siprofloksasin Grubu Antibiyotiklere Karşı Direncin Belirlenmesi

Abstract

G ünümüzde geniş spekturumlu antibiyotiklerin yaygın kullanımına bağlı olarak Pseudomonas aeruginosa’ da yüksek oranda antibiyotik direnci gelişmektedir. Böylece etken olduğu enfeksiyonların tedavisinde zorluklar yaşanmaktadır. Çalışmamızda Ankara’ daki üç farklı hastaneden izole edilen P. aeruginosa suşlarının beta-laktam, aminoglikozid ve siprofloksasin grubu antibiyotiklere karşı olan dirençlilikleri incelenerek çoklu ilaç dirençli suşlar ve antibiyotip grupları belirlendi. Böylece etkeni P. aeruginosa olan enfeksiyonların tedavisinde kullanılabilecek en etkili antibiyotiklerin belirlenmesi amaçlandı. 69 P. aeruginosa suşu ile aztreonam, meropenem, imipenem, amikasin, tobramisin, piperasilin, seftazidim, sefepim ve siprofloksasine karşı direnç durumları incelendi. P. aeruginosa suşlarında en yüksek direnç oranın aztreonama %66.6 karşı olduğu görülürken, en etkili antibiyotiğin amikasin %5.7 olduğu belirlendi. Ayrıca, P. aeruginosa suşlarında %36 oranında çoklu ilaç direnci saptandı. Bu bağlamda, aminoglikozid ve siprofloksasinin kombine antibiyotik tedavisinin dirençli P. aeruginosa suşlarında etkili olacağı düşünülmektedir

Keywords

Pseudomonas aeruginosa, Antibiyotik direnci, Antibiyotip, Çoklu ilaç direnci

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