Investigation of The Effects of Some Edible Mushroom Extracts on Human Carbonic Anhydrase Isozymes

Year 2015, Volume: 43 Issue: 3, 187 - 193, 01.09.2015

Hülya Karaçam Ekrem Tunca Yasemin Kaygısız Gözde İmdat Metin Bülbül

Abstract

In this study the effects of four edible mushroom species Lentinula edodes, Lactarius deliciosus, Lactarius deterrimus, Terfezia boudieri on human carbonic anhydrase isozymes hCA I and hCA II were investiga- ted as in vitro. hCA I and II isozymes were purified from erythrocytes by using Sepharose®4B-L-tyrosine-p- aminobenzene sulfonamide affinity chromatography. Later the effects of the mushroom extracts on the hydra- tase and esterase activities of hCA I and II were measured. L. edodes showed inhibitory effect on the esterase activities of hCA I and II. However, other extracts showed activator effects on the esterase activities of hCA I and II.

Keywords

Carbonic anhydrase, hydratase activity, esterase activity, mushroom extracts

Bazı Yenebilir Mantar Ekstrelerinin İnsan Karbonik Anhidraz İzozimleri Üzerine Etkilerinin İncelenmesi

Abstract

B u çalışmada, dört yenebilir mantar türünün Lentinula edodes, Lactarius deliciosus, Lactarius deterrimus, Terfezia boudieri insan karbonik anhidraz izoenzimleri hCA I ve hCA II üzerine etkileri in vitro olarak incelenmiştir. hCA I ve II izoenzimleri Sepharose®4B-L-tirozin-p-aminobenzen sülfonamit afinite kromatografisi kullanılarak eritrositlerden saflaştırıldı. Daha sonra mantar özütlerinin hCA I ve II izoenzimlerinin hidrataz ve esteraz aktivitelerinin üzerine etkileri ölçüldü. L. edodes hCA I ve II nin esteraz aktiviteleri üzerinde inhibitor etki göstermiştir. Ancak diğer özütler hCA I ve II nin esteraz aktiviteleri üzerinde aktivatör etki göstermişlerdir

Keywords

Karbonik anhidraz, hidrataz aktivitesi, esteraz aktivitesi, mantar özütleri

References

• C. Sarikurkcu, B. Tepe, M. Yamac, Evaluation of the antioxidant activity of four edible mushrooms from the Central Anatolia, Eskisehir Turkey: Lactarius deterrimus, Suillus collitinus, Boletus edulis, Xerocomus chrysenteron, Bioresource Technol., 99 (2008) 6651.
• A. Hamza, N. Zouari, S. Zouari, H. Jdir, S. Zaidi, M. Gtari, M. Neffati, Nutraceutical potential, antioxidant and antibacterial activities of Terfezia boudieri Chatin, a wild edible desert truffle from Tunisia arid zone, Arabian J. Chem., In press (2013) http://dx.doi. org/10.1016/j.arabjc.2013.06.015. Cólus, Shiitake (Lentinula edodes (Berkeley) Pegler) extracts as a modulator of micronuclei induced in Hep-2 cells, Toxicol. in Vitro, 20 (2006) 1555. stimulating of a new heteropolysaccharide from Lentinula edodes, J. Agric. Food Chem., 60 (2012) 11560.
• C.S.G. Kitzberger, A. Smânia Jr., R.C. Pedrosa, S.R.S. Ferreira, Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids, J. Food Eng., 80 (2007) 631.
• E. Baba, G. Uluköy, C. Öntaş, Effects of feed supplemented with Lentinula edodes mushroom extract on the immune response of rainbow trout Oncorhyncus mykiss, and disease resistance against Lactococcus garviae, Aquaculture, 448 (2015) 476- 482.
• D. Onbaşılı, G. Yuvalı Çelik, H. Katırcıoğlu, İ. Narin, Total phenolic, total flavonoid content and the DPPH free radical scavenging activity of Lactarius deliciosus (L.), Curr. Opin. Biotechnol., 24 (2013) 113-113.
• C. Tănase, L. Odochian, N. Apostolescu, A. Pui, TG- FTIR analysis applied to the study of thermal behavior of some edible mushrooms, J. Therm. Anal. Calorim., 103 (2011) 1079.
• R.F. Miambo, M. Laronze-Cochard, A.M. Lawson, R. Guillot, B. Baldeyrou, A. Lansiaux, C.T. Supuran, J. Sapi, Synthesis of new biologically active isothiazolo[4,5-b] carbazole-type tetracyclic derivatives via an indole- 2,3-quinodimethane approach, Tetrahedron, 70 (2014) 8286.
• C.T. Supuran, A. Scozzafava, Carbonic anhydrases as targets for medicinal chemistry, Bioorg. Med. Chem., 15 (2007) 4336.
• S.B. Öztürk Sarikaya, F. Topal, M. Şentürk, İ. Gülçin, C.T. Supuran, In vitro inhibition of a-carbonic anhydrase isozymes by some phenolic compounds, Bioorg. Med. Chem. Lett., 21 (2011) 4259.
• C.T. Supuran, Carbonic anhydrases: novel therapeutic applications for inhibitors and activators, Nature. Rev. Drug Discov., 7 (2008) 168.
• T.A. Coban, Ş. Beydemir, İ. Gülcin, D. Ekinci, A. Innocenti, D. Vullo, C.T. Supuran, Sildenafil is a strong activator of mammalian carbonic anhydrase isoforms I-XIV, Bioorg. Med. Chem., 17 (2009) 5791.
• I. Basaran, S. Sinan, U. Cakir, M. Bulut, O. Arslan, O. Ozensoy, In vitro inhibition of cytosolic carbonic anhydrases I and II by some new dihyroxycoumarin compounds, J. Enzym. Inhib. Med. Ch., 23 (2008) 32.
• C.T. Supuran, A. Scozzafava, Applications of carbonic anhydrase inhibitors and activators in therapy, Expert Opin. Ther. Pat., 12 (2002) 217.
• E.E. Rickli, S.A. Ghazanfar, B.H. Gibbons, J.T. Edsall, Carbonic anhydrases from human erythrocytes, Preparation and properties of two enzymes, J. Biol. Chem., 239 (1964) 1065.
• M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding, Anal. Biochem. 72 (1976) 248.
• U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227 (1970) 680.
• K.M. Wilbur, N.G. Anderson, Electrometric and colorimetric determination of carbonic anhydrase, J. Biol. Chem., 176 (1948) 147.
• J.A. Verpoorte, S. Mehta, J.T. Edsall, Esterase activities of human carbonic anhydrases B and C, J. Biol. Chem., 242 (1967) 4221.