The Role of Different Metal and Heavy Metal Ions on Chromium Reduction by Pseudomonas mendocina DS0601- FX-P22 ​

Year 2015, Volume: 43 Issue: 4, 295 - 300, 01.11.2015

Nazime Mercan Doğan Eda Evgen Gülümser Acar Doğanlı Göksel Doğan

Abstract

In this study, the effects of different metal ions such as Mn2+, Cu2+, Fe2+, Ba2+, Al3+, Ni2+, Co2+, Zn2+, Cd2+ and Pb2+ on the bacterial chromium reduction by Pseudomonas mendocina DS0601-FX-P22 were investigated. Two dif- ferent initial chromium concentrations 15 mg/L and 25 mg/L Cr VI were studied. The Cr VI reduction ability of the bacterium increased in the presence of metal ions like Cu2+ and Fe2+ and was significantly inhibited in the presence of metal ions like Ba2+ and Ni2+. Also, Cu2+ was the most efficient metal ion at Cr VI reduction for both Cr VI concentration 15 and 25 mg/L in P. mendocina DS0601-FX-P22 bacterium.

Keywords

Bioremediation, Chromate reduction, Heavy metal, Pseudomonas

Pseudomonas mendocina DS0601-FX-P22’nın Krom İndirgemesi Üzerine Farklı Metal ve Ağır Metallerin Rolü

Abstract

B u çalışmada, Mn2+, Cu2+, Fe2+, Ba2+, Al3+, Ni2+, Co2+, Zn2+, Cd2+ and Pb2+ gibi farklı metal iyonlarının Pseudomonas mendocina DS0601-FX-P22 tarafından bakteriyel krom indirgeme üzerine etkisi araştırılmıştır. İki farklı krom derişiminde çalışılmıştır 15 mg/L ve 25 mg/L Cr VI . Cu2+ ve Fe2+ gibi metal iyonlarının varlığında bakterinin Cr VI indirgeme yeteneği artmış, Ba2+ and Ni2+ gibi metal iyonlarının varlığında önemli derecede inhibe olmuştur. Ayrıca, P. mendocina DS0601-FX-P22 bakterisinin her iki Cr VI derişimi 15 ve 25 mg/L için Cr VI indirgemesinde en etkili metal iyonu Cu2+ olarak bulunmuştur

Keywords

Biyoremediasyon, Krom indirgeme, Ağır metal, Pseudomonas

References

• 1. B. Dhal, H.N. Thatoi, N.N. Das, B.D. Pandey, Chemical and microbial remediation of hexavalent chromium from contaminated soil and mining/metallurgical solid waste: A review, J. Hazard. Mater., 250-251 (2013) 272-291.
• 2. K. Salnikow, A. Zhitkovich, Genetic and epigenetic mechanisms in metal carcinogenesis and cocarcinogenesis: nickel, arsenic, and chromium, Chem. Res. Toxicol., 21 (2008) 28-44.
• 3. J. Kotas, Z. Stasicka, Chromium occurrence in the environment and methods of its speciation, Environ. Poll., 107 (2000) 263-283.
• 4. C. Desai, K. Jain, D. Madamwar, Evaluation of In vitro Cr(VI) reduction potential in cytosolic extracts of three indigenous Bacillus sp. isolated from Cr(VI) polluted industrial landfill, Biores. Technol., 99 (2008) 6059-6069.
• 5. V. Gomez, M.P. Callao, Chromium determination and speciation since 2000, Trend. Anal. Chem., 25 (2006) 1006-1015.
• 6. A.N. Mabbett, D. Sanyahumbi, P. Yong, L.E. Macaskie, Biorecovered precious metals from industrial wastes: Single-step conversion of a mixed metal liquid waste to a bioinorganic catalyst with environmental application, Environ. Sci. Techn. 40 (2006) 1015-1021.
• 7. A. Zahoor, A. Rehman, Isolation of Cr(VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater, J. Environ. Sci., 21 (2009) 814-820.
• 8. M. He, X. Li, H. Liu, S.J. Miller, G. Wang, C. Rensing, Characterization and genomic analysis of a highly chromate resistant and reducing bacterial strain Lysinibacillus fusiformis ZC1, J. Hazard. Mater., 185 (2011) 682-688.
• 9. Y. Cheng, F. Yan, F. Huang, W. Chu, D. Pan, Z. Chen, et al. Bioremediation of Cr(VI) and immobilization as Cr(III) by Ochrobactrum anthropi, Environ. Sci. Techn., 44 (2010) 6357-6363.
• 10. R. Boopathy, Factors limiting bioremediation Technologies, Biores. Technol., 74 (2000) 63-67.
• 11. Y.G. Liu , W.H. Xu, G.M. Zeng, X. Li, H. Gao, Cr(VI) reduction by Bacillus sp. isolated from chromium landfill, Process Biochem., 4 (2006) 1981-1986.
• 12. L. Xu, M. Luo, W. Li, X. Wei, K. Xie, L. Liu, C. Jiang, H. Liu, Reduction of hexavalent chromium by Pannonibacter phragmitetus LSSE-09 stimulated with external electron donors under alkaline conditions, J. Hazard. Mater., 185 (2011) 1169-1176.
• 13. D. Long, X. Tang, K. Cai, G. Chen, L. Chen, D. Duan, J. Zhu, Y. Chen, Cr(VI) reduction by a potent novel alkaliphilic halotolerant strain Pseudochrobactrum saccharolyticum LY10, J. Hazard. Mater., 256–257 (2013) 24–32.
• 14. N.M. Dogan, C. Kantar, S. Gulcan, C.J. Dodge, B.C. Yilmaz, M.A. Mazmanci, Chromium(VI) Bioremoval by Pseudomonas Bacteria: Role of Microbial Exudates for Natural Attenuation and Biotreatment of Cr(VI) Contamination, Environ. Sci. Technol. 45(6), (2011) 2278-2285.
• 15. G. Acar, N.M. Dogan, E. Evgen, G. Dogan, Cr(VI) Reduction by Bacillus licheniformis B22 Isolated From Pamukkale Thermal Region, Current Opinion in Biotechnol., 22S, S15-S152 (2011) 69-70.
• 16. G.A. Doganlı, N.M. Dogan, Reduction of Cr(VI) to Cr(III) by thermal Bacillus licheniformis B22 under different temperatures using binary and ternary combinations of organic acids, Desalin. Water Treat. 52 (2014) 7163–7171.
• 17. American Public Health Association (APHA). 1995. Standard Methods for the Examinations of Water and Wastewater. 19th ed.; Washington, DC.
• 18. F.A.O. Camargo, B.C. Okeke, F.M. Bento, W.T. Frankenberger, In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES 29 stimulated by Cu2+, Appl.Microbiol. Biotechnol., 62 (2003) 569-573.
• 19. J. McLean, T.J. Beveridge, Chromate reduction by a Pseudomonad isolated from a site contaminated with chromated copper arsenate, Appl. Environ. Microbiol. 67 (2001) 1076-1084.
• 20. S. Sultan, S. Hasnain, Reduction of toxic hexavalant chromium by Ochrobacterium intermedium strain SDCr-5 stimulated by heavy metals, Biores. Technol., 98(2) (2007) 340-344.
• 21. A. Pal, A.K. Paul, Aerobic Chromate Reduction by Chromium-resistant Bacteria Isolated from Serpentine Soil, Microbiol. Res., 159 (2004) 347-354.
• 22. A. Pal, S. Dutta, A.K. Paul, Reduction of hexavalent chromium by cell free extract of Bacillus sphaericus AND 303 isolated from serpentine soil, Current Microbiol., 51 (2005) 327-30.