Development of a Flow-Injection with Spectrophotometric Method for Total Iron in Water Samples

Year 2017, Volume: 45 Issue: 4, 515 - 522, 01.11.2017

Adem Asan Bediha Akmeşe

Abstract

Asimple spectrophotometric flow-injection method is reported for the highly sensitive and fast determina- tion of iron III and total iron. The method is based on the formation of iron III - 1-nitroso-2-naphthol-3,6 sulphonate NRS complex. The optimum conditions for the reaction of iron III with NRS is studied. And the complex is selectively monitored at λmax370 nm. With the reagent carrier solvent used was 5x10-4 M 1-nitroso-2-naphthol-3,6 sulphonate in 0.1 M acetic acid-acetate buffer solution at pH 4.0. Parameters affect simultaneously for the determination of iron III and interfering ions were tested. Relative standard deviation for five consecutive injections of 70 μg.L-1 iron III was 1.46% and for 5 μg.L-1 iron III , it was 3.12%. The calibration graphwaslinearintheiron III concentrationrange0.1-100μg.L-1 andthelimitofdetectionwas0.05μg.L-1 for a 20 μL injection volume. The precision and accuracy of the method were checked by analysis of certified reference material and AAS method. The method was successfully applied to determine trace amounts of iron III and total iron in river and sea water samples.

Keywords

Flow-Injection Analysis, iron, Spectrophotometric Determination, 1-Nitroso-2-naphthol-3, 6 sulphonate

Su Örneklerinde Toplam Demir Tayini için Spektrofotometrik Akış Enjeksiyon Yöntemi Geliştirilmesi

Abstract

D emir III ve toplam demirin oldukça hassas ve hızlı tayin edilmesi için basit bir spektrofotometrik akış enjeksiyon yöntemi geliştirilmiştir. Yöntem, demir III ile 1-nitrozo-2-naftol-3,6 sülfonat NRS reaktifi arasında oluşan gelen kompleksin oluşumuna dayanmaktadır. Demir III ile NRS tepkmesi için optimum koşullar belirlenmiş ve kompleks λmax370 nm’de spesifik absorpsiyon dalga boyuna sahip olduğu görülmüştür. Hareketli faz olarak pH 4.0’de 0.1 M asetik asit-asetat tampon çözeltisinde 5x10-4 M derişimde l-nitrozo-2-naftol-3,6 sülfonat tuzu kullanılmıştır. Demir III tayininde kullanılan parametreler ve girişim yapan iyonlar test edildi. Peş peşe yapılan 70 µg.L-1 derişimdeki demir III enjeksiyonu için bağıl standart sapma %1.46 ve 5 µg L-1 derişimdeki demir III için ise elde edilen bağıl standart sapma %3.12 olarak belirlendi. Doğrusal çalışma bölgesinin 0.1-100 μg.L-1 demir III derişim aralığında olduğu belirlendi ve 20 μL enjeksiyon hacmi için tayin sınırı 0.05 μg.L-1 olarak bulundu. Yöntemin kesinliği ve doğruluğu, standart referans madde ve AAS yöntemi ile analiz edilerek test edildi. Yöntem, nehir ve deniz suyu numunelerinde demir III ve toplam demirin eser miktarlarını belirlemek için başarıyla uygulanmıştır

Keywords

Akış-Enjeksiyon Analizi, Demir, Spektrofotometrik Tayin, l-Nitrozo-2-naftol-3, 6 sülfonat.

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