Bioactive Compound Activity Inducement of Thermophile Cyanobacterium aponinum Under Stress Conditions

Yıl 2021, , 233 - 242, 17.05.2021

Nur Koçberber Kılıç , Gönül Dönmez

https://doi.org/10.15671/hjbc.696762

Cited By: 4

Öz

Bu çalışmada, Türkiye'de kaplıcalardan izole edilen 7 termofil siyanobakterinin biyoaktif özellikleri araştırılmıştır. Bunlardan en yüksek antimikrobiyel aktiviteye sahip olan Suş H2, Cyanobacterium aponium olarak tanılanmıştır. Siyanobakteriyel biyokütlenin biyoaktif karakteri, farklı azot konsantrasyonları (0.5 g/L, 1.0 g/L, 1.5 g/L ve 2.0 g/L), ışık yoğunlukları (1200lx, 2400 lx, 3600 lx ve 4800 lx), inkübasyon süreleri (7 gün, 14 gün, 21 gün ve 28 gün) ve sıcaklıklar (30 °C, 40 °C, 45 °C ve 50 °C) açısından araştırılmıştır. Siyanobakteriler tarafından üretilen biyoaktif maddelerin etkinliğinin stres koşulları tarafından tetiklendiği gözlenmiştir. C. aponinum yüksek ışık yoğunluğuna veya sıcaklığa maruz kaldığında, siyanobakteriler test edilen diğer çevresel koşullardan daha verimli biyoaktif bileşikler üretmiştir. En yüksek antimikrobiyel aktivite, siyanobakteri 1.0 g/L azot içeren bir ortamda, 3600 lx ışık şiddeti altında, 45 °C'de 14 gün boyunca inkübasyondan sonra elde edilen biyokütleden alınan ekstraktlar ile E. coli 0157: H7 ATCC 35150'ye karşı bulunmuştur. Bu çalışmada ilk kez böyle bir yaklaşımla, termofilik C. aponinum tarafından biyoaktif bileşiklerin üretilmesi ve en etkin biyoaktif bileşikleri elde etmek için çevresel koşulların optimizasyonu araştırılmıştır.

Anahtar Kelimeler

Bioactive compounds, Cyanobacterium aponinum, thermophile, stress

Kaynakça

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