Effect Of α- Tocopherol and Ascorbic Acid on the Genotoxicity by Gamma-Irradiation in Drosophila melanogaster

Yıl 2022, Cilt: 50 Sayı: 1, 93 - 98, 05.01.2022

Güzin Emecen , Hacer Ünlü

https://doi.org/10.15671/hjbc.932240

Cited By: 1

Öz

Toksik olmayan ve etkili doğal antioksidanların radyoprotektif etkilerinin belirlenmesi önemli bir konudur. E Vitamini, radyasyon ile oluşan serbest radikalleri etkili bir şekilde temizlemesiyle bilinmektedir. Bununla birlikte, C vitamini konsantrasyona bağlı olarak prooksidan veya antioksidan görevi görebilir.
Bu çalışmada, Drosophila melanogaster’de gama ışınlamasına karşı E ve C vitamini uygulamasının (ayrı ayrı ve birlikte) antimutajenik etkilerini araştırdık. Bu amaçla, SLRL ve Translokasyon genetik toksikoloji testlerini kullandık. Her iki test sisteminde, sadece erkek bireylere radyasyon ve vitamin uygulamaları yapıldı. Bu erkekler, daha önce vitamin ve radyasyon almamış dişilerle çaprazlandı ve radyasyon yoluyla üretilen letal kromozomları taşıyan bireyleri belirlemek için bu erkeklerin yavruları tarandı. Ardından, vitamin uygulamasının, radyasyonla oluşmuş letal kromozom taşıyan birey sayısını azaltıp azaltmadığını araştırdık. Sonuçlar, E vitamininin letal frekansını yalnızca tek başına ve ışınlamadan önce uygulandığında önemli ölçüde azalttığını göstermiştir. C vitamini hiçbir şekilde önleyici etki göstermemiştir. C ve E vitaminlerinin birlikte verilmesinin, kontrole kıyasla letaliteyi azaltmadığı tespit edilmiştir.

Anahtar Kelimeler

Drosophila, genotoxicity, radioprotection, vitamin E, vitamin C

Teşekkür

We would like to thank Prof. Dr. Dilek Şolpan at Department of Chemistry, Hacettepe University for her helps during the irradiation assay and Associate Professor Pınar Emecen Huja for her valuable assistance during the experiments.

Kaynakça

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