Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches

Yıl 2023, Cilt: 51 Sayı: 3, 297 - 307, 16.07.2023

Elmas Gökoğlu Buse Aklar Tugba Taskın Tok

https://doi.org/10.15671/hjbc.1272350

Öz

The binding properties of insulin hormone to the potential antidiabetic coumarin derivative umbelliferone (7hydroxycoumarin, 7HC) was investigated by absorption, fluorescence quenching and molecular docking methods. The negative signs of thermodynamic parameters (H and S) indicated that hydrogen bonds and van der Waals forces were dominant in the binding mode. The effect of common metal ions was investigated on binding parameters. According to the Förster’s theory; binding distance, r was obtained as 4.17 nm. The spectral data further supported by molecular docking calculations which show hydrogen bonds between 7HC and insulin.

Anahtar Kelimeler

Insulin, fluorescence quenching, thermodynamic parameter, FRET

Destekleyen Kurum

Hacettepe University Scientific Research Fund

Proje Numarası

FBA2017-12854

Kaynakça

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