Effects of waiting time between trials and water temperature on cognitive functions, body temperature and body weight in rats in Morris water maze

Yıl 2022, Cilt: 6 Sayı: 2, 70 - 75, 31.08.2022

Duygu Sultan Oran , Zeynep Yıldız , Arzu Temizyürek , Ayşin Selcan

https://doi.org/10.30704/http-www-jivs-net.1115170

Öz

The Morris water maze (MWM) is a widely used test among neurobiologists to measure spatial memory. The implementation of this test carries the risk of hypothermia periods in animals. The level of hypothermia may affect age-related memory processes as a significant factor. The occurrence of hypothermia throughout the MWM protocol should be better understood as hypothermia may impair memory performance. Ensuring the standardization of the experiments and minimizing side effects require a detailed examination of the hypothermia-related processes. Our study aims to replicate and extend the data of previous studies in terms of determining the possible species-specific variations and provide data for reorganizing the time intervals. In this study, rats (Wistar Hannover) were used and grouped according to the differences in the inter-trial interval (ITI) (30-s and 13-min) and water temperatures (20 °C and 24 °C). The effects of ITI and water temperature on probe performance were analysed statistically (mixed two-way ANOVA). Results showed that the 13 minute waiting group of animals performed statistically better in the MWM probe phase compared to the 30 second waiting group. The prolongation of ITI between the tests was found to have a positive impact on the memory performance. Longer ITI should be preferred instead of the frequently used 30-60 second test intervals. Thus, animals will be exposed to less stress and more reliable results can be obtained, also possible side effects of hypothermia can be minimized while performing the MWM test.

Anahtar Kelimeler

comorbidity, hypothermia, memory, spatial, temperature

Teşekkür

The authors thank Dr. Ali Behram Salar for his contributions and suggestions for statistical analysis and visualization of the results.

Kaynakça

• Alam, A., Hana, Z., Jin, Z., Suen, K. C., & Ma, D. (2018). Surgery, neuroinflammation and cognitive impairment. EBioMedicine, 37, 547–556. https://doi.org/10.1016/j.ebiom.2018.10.021
• Bellmund, J. L. S., Gärdenfors, P., Moser, E. I., & Doeller, C. F. (2018). Navigating cognition: Spatial codes for human thinking. Science (New York, N.Y.), 362(6415), eaat6766. https://doi.org/10.1126/science.aat6766
• Bizon, J. L., Helm, K. A., Han, J. S., Chun, H. J., Pucilowska, J., Lund, P. K., & Gallagher, M. (2001). Hypothalamic-pituitary-adrenal axis function and corticosterone receptor expression in behaviourally characterized young and aged Long-Evans rats. The European Journal of Neuroscience, 14(10), 1739–1751. https://doi.org/10.1046/j.0953-816x.2001.01781.x
• Conn, P. M. (2011). Handbook of Models for Human Aging. Elsevier.
• D’Hooge, R., & De Deyn, P. P. (2001). Applications of the Morris water maze in the study of learning and memory. Brain Research. Brain Research Reviews, 36(1), 60–90. https://doi.org/10.1016/s0165-0173(01)00067-4
• Ecevitoglu, A., Soyman, E., Canbeyli, R., & Unal, G. (2020). Paw preference is associated with behavioural despair and spatial reference memory in male rats. Behavioural Processes, 180, 104254. https://doi.org/10.1016/j.beproc.2020.104254
• Eichenbaum, H. (2000). A cortical–hippocampal system for declarative memory. Nature Reviews Neuroscience, 1(1), 41–50. https://doi.org/10.1038/35036213
• Gallagher, M., Burwell, R. D., & Burchinal, M. (2015). Severity of Spatial Learning Impairment in Aging: Development of a Learning Index for Performance in the Morris Water Maze. Behavioral Neuroscience, 129(4), 540–548. https://doi.org/10.1037/bne0000080
• Golub, V. M., & Reddy, D. S. (2022). Contusion brain damage in mice for modelling of post-traumatic epilepsy with contralateral hippocampus sclerosis: Comprehensive and longitudinal characterization of spontaneous seizures, neuropathology, and neuropsychiatric comorbidities. Experimental Neurology, 348, 113946. https://doi.org/10.1016/j.expneurol.2021.113946
• Hamm, R. J. (1981). Hypothermia-induced retrograde amnesia in mature and aged rats. Developmental Psychobiology, 14(4), 357–364. https://doi.org/10.1002/dev.420140408
• Iivonen, H., Nurminen, L., Harri, M., Tanila, H., & Puoliväli, J. (2003). Hypothermia in mice tested in Morris water maze. Behavioural Brain Research, 141(2), 207–213. https://doi.org/10.1016/S0166-4328(02)00369-8
• Kim, J. J., Lee, H. J., Welday, A. C., Song, E., Cho, J., Sharp, P. E., Jung, M. W., & Blair, H. T. (2007). Stress-induced alterations in hippocampal plasticity, place cells, and spatial memory. Proceedings of the National Academy of Sciences of the United States of America, 104(46), 18297–18302. https://doi.org/10.1073/pnas.0708644104
• Li, L., Li, Z., Cao, Y., Fan, D., Chui, D., & Guo, X. (2016). Increased extrasynaptic GluN2B expression is involved in cognitive impairment after isoflurane anesthesia. Experimental and Therapeutic Medicine, 12(1), 161–168. https://doi.org/10.3892/etm.2016.3306
• Lindner, M. D., & Gribkoff, V. K. (1991). Relationship between performance in the Morris water task, visual acuity, and thermoregulatory function in aged F-344 rats. Behavioural Brain Research, 45(1), 45–55. https://doi.org/10.1016/s0166-4328(05)80179-2
• Mansvelder, H. D., Verhoog, M. B., & Goriounova, N. A. (2019). Synaptic plasticity in human cortical circuits: Cellular mechanisms of learning and memory in the human brain? Current Opinion in Neurobiology, 54, 186–193. https://doi.org/10.1016/j.conb.2018.06.013
• Morris, R. (1984). Developments of a water-maze procedure for studying spatial learning in the rat. Journal of Neuroscience Methods, 11(1), 47–60. https://doi.org/10.1016/0165-0270(84)90007-4
• O’Keefe, J., & Nadel, L. (1979). Précis of O’Keefe & Nadel’s The hippocampus as a cognitive map. Behavioral and Brain Sciences, 2(4), 487–494. https://doi.org/10.1017/S0140525X00063949
• Pezük, P., Göz, D., Aksoy, A., & Canbeyli, R. (2006). BNST lesions aggravate behavioral despair but do not impair navigational learning in rats. Brain Research Bulletin, 69(4), 416–421. https://doi.org/10.1016/j.brainresbull.2006.02.008
• Rauch, T. M., Welch, D. I., & Gallego, L. (1989). Hyperthermia impairs retrieval of an overtrained spatial task in the Morris water maze. Behavioral and Neural Biology, 52(3), 321–330. https://doi.org/10.1016/s0163-1047(89)90442-1
• Scoville, W. B., & Milner, B. (1957). Loss of recent memory after bilateral hippocampal lesions. Journal of Neurology, Neurosurgery, and Psychiatry, 20(1), 11–21.
• Vorhees, C. V., & Williams, M. T. (2006). Morris water maze: Procedures for assessing spatial and related forms of learning and memory. Nature Protocols, 1(2), 848–858. https://doi.org/10.1038/nprot.2006.116
• Xu, G., Li, T., & Huang, Y. (2022). The Effects of Intraoperative Hypothermia on Postoperative Cognitive Function in the Rat Hippocampus and Its Possible Mechanisms. Brain Sciences, 12(1), 96. https://doi.org/10.3390/brainsci12010096