Düzce İli'nden Elde Edilen Çeşitli Bitki Ekstraktlarının Clavibacter michiganensis subsp. michiganensis'e Karşı Antibakteriyel Aktivitesi

Öz

Clavibacter michiganensis subsp. michiganensis (Cmm) kaynaklı domates bakteriyel kanseri, %60'a varan verim kayıplarına yol açabilen ve bakır bazlı bakterisitlere karşı gelişen direnç ile çevresel sorunlar nedeniyle mücadelesi giderek zorlaşan bir hastalıktır. Bu çalışma; çevre dostu ve yerel kaynaklı alternatifler belirlemek amacıyla, Düzce ilinden toplanan 18 bitki türünün etanolik ekstraktlarının Cmm'ye karşı in vitro antibakteriyel aktivitelerini 10, 20 ve 30 mg/mL konsantrasyonlarda agar kuyu difüzyon yöntemi kullanarak değerlendirmiştir. Bitki ekstraktları ve konsantrasyonları arasında antibakteriyel etkinlik bakımından önemli farklılıklar gözlemlenmiştir. Tüm konsantrasyonlarda en güçlü inhibitör etkiyi Nane (Mentha × piperita L.) göstermiş; onu sırasıyla Siyah Üzüm (Vitis vinifera L.), Defne (Laurus nobilis L.), Okaliptüs (Eucalyptus globulus Labill.) ve Aloe Vera (Aloe vera (L.) Burm.f.) izlemiştir. Limon (Citrus limon (L.) Osbeck), Üzüm Çekirdeği (Vitis vinifera L. semen) ve Zencefil (Zingiber officinale Roscoe) gibi bazı ekstraktlar doza bağlı aktivite gösterirken, Çay Ağacı (Melaleuca alternifolia (Maiden & Betche) Cheel) ve Pelin Otu/Sivri Otu (Artemisia abrotanum L.) en düşük antibakteriyel etkiyi sergilemiştir. Bu bulgular, yerel kaynaklı botanik ekstraktların domates bakteriyel kanseriyle mücadelede geleneksel bakır bazlı bakterisitlere sürdürülebilir bir alternatif olma potansiyelini vurgulamaktadır. Minimum inhibitör konsantrasyonlarının belirlenmesi, aktif bileşiklerin izole edilmesi ve entegre hastalık yönetimi uygulamaları için in vivo etkinliğin değerlendirilmesi amacıyla ileri araştırmalar önerilmektedir.

Anahtar Kelimeler

• Bitki ekstraktı
• Antibakteriyel aktivite
• Biyokontrol
• Domates bakteriyel kanseri

Etik Beyan

Bu çalışma; insan katılımcılar, klinik örnekler veya hayvan deneyleri içermediği için etik kurul onayı gerektirmemektedir.

Antibacterial Activity of Various Plant Extracts Obtained From Duzce Province Against Clavibacter michiganensis subsp. michiganensis

Öz

Tomato bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis (Cmm), can result in yield losses of up to 60% and is increasingly difficult to manage due to environmental concerns and resistance associated with copper based bactericides. To identify ecofriendly and locally sourced alternatives, this study evaluated the in vitro antibacterial activity of ethanolic extracts from 18 plant species collected in Düzce province against Cmm using the agar well diffusion method at concentrations of 10, 20, and 30 mg/mL. Significant variation in antibacterial efficacy was observed among plant extracts and concentrations. Peppermint (Mentha × piperita L.) exhibited the strongest inhibitory effect across all concentrations, followed by Black Grape (Vitis vinifera L.), Bay Laurel (Laurus nobilis L.), Eucalyptus (Eucalyptus globulus Labill.), and Aloe Vera (Aloe vera (L.) Burm.f.). Some extracts, such as Lemon (Citrus limon (L.) Osbeck), Grape Seed (Vitis vinifera L. semen), and Ginger (Zingiber officinale Roscoe), showed dose-dependent activity, while Tea Tree (Melaleuca alternifolia (Maiden & Betche) Cheel) and Southernwood (Artemisia abrotanum L.) had the lowest antibacterial effects. These findings highlight the potential of locally sourced botanical extracts as sustainable alternatives to conventional copper based bactericides for managing tomato bacterial canker. Further research is recommended to determine minimum inhibitory concentrations, isolate active compounds, and assess in vivo efficacy for integrated disease management applications.

Anahtar Kelimeler

• Plant extract
• antibacterial activity
• biocontrol
• tomato bacterial canker

Etik Beyan

This study does not require ethical approval as it does not involve human participants, clinical samples, or animal experiments

Kaynakça

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