Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract
Öz
Relying on the previous literature, Albizia amara possesses phytochemical constituents having significant pharmacological activities. Our study preliminary screened the phytochemical constituents and investigated the potential anticonvulsant activity using the Pilocarpine-induced seizures on albino rats. The qualitative phytochemical screening of the extract indicated the presence of flavonoids, alkaloids, saponins, tannins, and sterols. The anticonvulsant activity assessment revealed that the extract (400 mg/kg I.P.) increased the latency to the first seizure and significantly decreased the duration of the seizure. Moreover, the extract reduced the mortality rate during the first observed 24 hours. Furthermore, to predict the probable mechanism of action, in silico study was conducted. The results indicated that predicted compounds that have the highest contribution in the activity are Budmunchiamine A, 1, 2-benzenedicarboxylic acid, mono (2-Ethylhexyl) ester, Hexadecanoic acid methyl ester, and Pithecolobine. The predicted targets that involved in the antiepileptic activity are neural acetylcholine receptor subunits alpha-4 and alpha-7, 5-hydroxytryptamine receptor 3A, Cannabinoid receptor 1, kinase C gamma & epsilon subunits, and Glutamate carboxypeptidase-2 enzyme. Unfortunately, Budmunchiamine A was predicted to be Cardiotoxic and Hepatotoxic. Pithecolobine was predicted to be Cardiotoxic. In conclusion, the findings demonstrate the potentiality of A. amara’s ethanolic leaves extract in the attenuation of Pilocarpine induced convulsion.
Anahtar Kelimeler
Albizia amara; Phytochemical screening Anticonvulsant activity Pilocarpine-induced seizure silico study
Kaynakça
- 1 Wahab, A. Difficulties in Treatment and Management of Epilepsy and Challenges in New Drug Development. Pharmaceuticals (Basel). 2010;3: 2090-2110.
- 2 McCagh, J. in Epilepsy Topics (ed Mark D. Holmes) (July 16th 2014).
- 3 JA, D. Mechanisms of action of antiepileptic drugs. Seizure. 1995;4, 267-271.
Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract
Öz
Relying on the previous literature, Albizia amara possesses phytochemical constituents having significant pharmacological activities. Our study preliminary screened the phytochemical constituents and investigated the potential anticonvulsant activity using the Pilocarpine-induced seizures on albino rats. The qualitative phytochemical screening of the extract indicated the presence of flavonoids, alkaloids, saponins, tannins, and sterols. The anticonvulsant activity assessment revealed that the extract (400 mg/kg I.P.) increased the latency to the first seizure and significantly decreased the duration of the seizure. Moreover, the extract reduced the mortality rate during the first observed 24 hours. Furthermore, to predict the probable mechanism of action, in silico study was conducted. The results indicated that predicted compounds that have the highest contribution in the activity are Budmunchiamine A, 1, 2-benzenedicarboxylic acid, mono (2-Ethylhexyl) ester, Hexadecanoic acid methyl ester, and Pithecolobine. The predicted targets that involved in the antiepileptic activity are neural acetylcholine receptor subunits alpha-4 and alpha-7, 5-hydroxytryptamine receptor 3A, Cannabinoid receptor 1, kinase C gamma & epsilon subunits, and Glutamate carboxypeptidase-2 enzyme. Unfortunately, Budmunchiamine A was predicted to be Cardiotoxic and Hepatotoxic. Pithecolobine was predicted to be Cardiotoxic. In conclusion, the findings demonstrate the potentiality of A. amara’s ethanolic leaves extract in the attenuation of Pilocarpine induced convulsion.
Anahtar Kelimeler
Albizia amara phytochemical screening anticonvulsant activity Pilocarpine-induced seizures insilico study
Kaynakça
- 1 Wahab, A. Difficulties in Treatment and Management of Epilepsy and Challenges in New Drug Development. Pharmaceuticals (Basel). 2010;3: 2090-2110.
- 2 McCagh, J. in Epilepsy Topics (ed Mark D. Holmes) (July 16th 2014).
- 3 JA, D. Mechanisms of action of antiepileptic drugs. Seizure. 1995;4, 267-271.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Eczacılık ve İlaç Bilimleri |
| Bölüm | Research Articles |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Ocak 2021 |
| Kabul Tarihi | 14 Mart 2021 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 41 Sayı: 1 |