Deplastinasyon Sonrası Plastine Böbreğin Histolojik Yapısı

Abstract

Plastinasyon, gelecekte araştırma ve eğitim amaçlı kullanılabilecek kalıcı kuru doku ve organ örnekleri elde etmeye yönelik bir laboratuvar işlemidir. Kısaca bu metodoloji silikon gibi sentetik maddelerin dehidrasyona ve dokuya nüfuz etmesine dayanmaktadır. Bu çalışmada ışık mikroskobu altında incelemek için önceden plastine edilmiş böbrekleri deplastine etmeyi amaçladık. Bu çalışmada 7 örnek kontrol ve 7 örnek plastinasyon-deplastinasyon (p/d) grubu olmak üzere, 14 koyun böbreği kullanıldı. Hem kontrol hem de p/d gruplarındaki böbrekler %10 formalin içinde sabitlendi. Kontrol grubundaki örnekler rutin doku işleme protokolü izlenerek parafine gömüldü, ancak p/d grubundaki örnekler alkol ve metil benzen içinde deplastine edildikten sonra parafine gömüldü. Parafin bloklarından elde edilen 5 μm kalınlığındaki kesitler Hematoksilen ve eozin (H&E) ile boyanarak ardından periyodik asit-schiff (PAS) ile ışık mikroskobu altında incelendi. Kontrol
grubunda normal histolojik yapılar gözlendi. P/d grup bloklarından kesitler elde etmek zor olduğu için küçük parçalar elde edildi. Morfolojik yapılarda bazı dejenarasyonlar gözlemlendi. Bu, plastine böbrek örneklerinin alkol ve metil benzen ile deplastinasyonunun ışık mikroskobu altında değerlendirilmesinde kısmen başarılı olabileceğini gösteren ilk çalışmadır. Ancak literatürdeki sonuçlarla karşılaştırıldığında böbreğin geniş parankiminden dolayı bazı kısıtlılıklarının olabileceğini düşünmekteyiz, ancak optimum protokolleri geliştirmek için daha fazla çalışmaya ihtiyaç vardır.

Keywords

• Böbrek
• histoloji
• koyun
• plastinasyon
• veteriner anatomi

Histological Structure of the Plastinated Kidney Following Deplastination

Abstract

Plastination is a laboratory process to obtain permanent dry tissue and organ sample. That can be used in the future for investigation and educational purposes. This methodology is based on dehydration and penetration of synthetic substances such as silicon into tissue. In this study, it was aimed to deplastinate previously plastinated kidneys in order to examine them under the light microscope. In this study, 14 sheep kidneys were used, seven samples of control and seven samples of plastination-deplastination (p / d) group. Kidneys in both control and p/d groups were fixed in 10% formalin. The samples in the control group were embedded in paraffin following routine tissue processing protocol. However, the samples in the p/d group were deplastinated in alcohol and methylbenzene and embedded into paraffin. 5 μm thick sections obtained from paraffin blocks were stained with hematoxylin and eosin (H&E), periodic acid-shiff (PAS) and then examined under the light microscope. Typical histological structures were observed in the control group. Small fragments were obtained as it was challenging to obtain sections from the P/d group blocks. Morphological structures were visible with some pseudo degenerations and wrong staining. This study is the first study that demonstrates alcohol and methylbenzene deplastination can be partially successful for evaluating plastinated kidney samples under a light microscope. However, we believe that the kidney may have limitations due to its wide parenchyma compared with literature conclusions. Nevertheless, more studies are required to develop the optimum protocols.

Keywords

• histology
• Kidney
• sheep
• plastination
• veterinary anatomy

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