Effects of Thymoquinone on Lipid Profile in Rats with Experimentally Acute Pancreatitis

Abstract

This study aimed to determine the possible effects of thymoquinone on lipid profile in rats with cerulein-induced acute pancreatitis. No application was made to the control group (K) during the study period of nine days. The thymoquinone group (TQ) animals were administered 20 mg/kg of thymoquinone daily for nine days. In the acute pancreatitis group (AP), acute pancreatitis was induced by intraperitoneal cerulein administration of 50 μg/kg and 2 hours later, 25 μg/kg on the seventh day of the study. Animals in the acute pancreatitis+thymoquinone group (AP+TQ) were intraperitoneally administered 20 mg/kg of thymoquinone daily for nine days. On the seventh day of the study, acute pancreatitis was induced by intraperitoneal administration of 50 μg/kg and 2 hours later 25 μg/kg of cerulein after 2 hours from thymoquinone administration. In blood samples taken from all animals, Lipase, Amylase, Triglyceride, Total Cholesterol, low-density lipoprotein (LDL-cholesterol), and high-density lipoprotein (HDL-cholesterol) levels were determined. The amylase and lipase levels of rats with acute experimental pancreatitis were significantly higher than the control group (p<0.05). It was determined that these enzyme levels, which were high in the acute pancreatitis group, were significantly suppressed in rats with acute pancreatitis treated with thymoquinone (p<0.05). The increase in plasma triglyceride and total cholesterol levels due to acute experimental pancreatitis induced with cerulein was significant compared to the control group (p<0.05). LDL-cholesterol level of rats with acute pancreatitis was found to be significantly higher than the control group (p<0.05), while HDL-cholesterol level was significantly lower (p<0.05). The total cholesterol and LDL-cholesterol levels of rats with acute pancreatitis treated with thymoquinone were significantly lower than those of the acute pancreatitis group (p<0.05). In conclusion, in light of differences in plasma lipid parameters and some enzyme levels, it was considered that thymoquinone has positive effects on acute pancreatitis.

Keywords

• Cerulein
• Thymoquinone
• Lipid Profile
• Rats

Deneysel Akut Pankreatit Oluşturulan Ratlarda Lipit Profil Üzerine Timokinonun Etkileri

Abstract

Çalışmada serulein ile akut pankreatit oluşturulan ratlarda timokinonun lipit profil üzerine olası etkilerinin belirlenmesi amaçlanmıştır. Çalışma süresi olan 9 gün boyunca kontrol grubuna (K) herhangi bir uygulama yapılmadı. Timokinon grubundaki (TQ) hayvanlara 9 gün süre ile günde 20 mg/kg timokinon intraperitoneal olarak verilirken, akut pankreatit grubundaki (AP) hayvanlarda araştırmanın 7. gününde 50 μg/kg ve 2 saat sonra 25 μg/kg seruleinin intraperitoneal olarak uygulanmasıyla akut pankreatit oluşturuldu. Akut pankreatit+timokinon grubunda ise (AP+TQ) günde 20 mg/kg olmak üzere 9 gün süre ile intraperitoneal olarak timokinon verilen hayvanlarda araştırmanın 7. gününde timokinon uygulamasından 2 saat sonra 50 μg/kg ve bundan 2 saat sonra 25 μg/kg seruleinin intraperitoneal olarak uygulanmasıyla akut pankreatit oluşturuldu. Bütün hayvanlardan alınan kan örneklerinde Lipaz, Amilaz, Trigliserit, Total Kolesterol, Düşük dansiteli lipoprotein (LDL-kolesterol) ve Yüksek dansiteli lipoprotein (HDL-kolesterol) düzeyleri belirlendi. Deneysel akut pankreatit oluşturulan ratlarda amilaz ve lipaz düzeyleri kontrol grubuna göre anlamlı olarak yüksek olduğu belirlendi (p<0.05). Akut pankreatit oluşturulan grupta yüksek olan bu enzim düzeylerinin timokinon uygulanan akut pankreatitli ratlarda önemli oranda baskılandığı belirlendi (p<0.05). Çalışmada serulein ile oluşturulan deneysel akut pankreatite bağlı olarak plazma trigliserit ve total kolesterol düzeylerinin kontrol grubuna göre önemli oranda yüksek olduğu belirlendi (p<0.05). Akut pankreatitli ratlarda LDL-kolesterol düzeyi kontrol grubuna göre anlamlı oranda yüksek bulunurken (p<0.05), HDL-kolesterol düzeyi ise anlamlı oranda düşüktü (p<0.05). Timokinon uygulanan akut pankreatitli ratların total kolesterol ve LDL-kolesterol düzeylerinin akut pankreatitli gruba göre anlamlı oranda düşük olduğu belirlendi (p<0.05). Sonuç olarak, plazma lipit parametreleri ve bazı enzim düzeylerindeki farklılıklar dikkate alındığında timokinonun akut pankreatit üzerine olumlu etkileri olduğu kanaatine varıldı.

Keywords

• Serulein
• Timokinon
• Lipit Profil
• Rat

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