Koç Spermasının Dondurulmasında Bireysel Farklılıkların Etkisi

Yıl 2017, , 79 - 83, 30.06.2017

Çiğdem Çebi Şen , Recai Kulaksız Ali Daşkın Ergun Akçay

https://doi.org/10.31196/huvfd.325767

Öz

Bu çalışma, koç spermasının dondurulmasında
bireysel farklılıkları ortaya koymak ve bu farklılık üzerine sulandırıcı etkisini
belirlemek amacıyla yapıldı. Ejakulatlar 5 Karayaka koçundan sezon içi
dönemde suni vajina yardımıyla haftada bir kez alındı. Sperma örnekleri bireysel olarak 4 farklı sperma sulandırıcısı (Yağsız süt tozu, Tris, Andromed, Bioxcell) ile
sulandırıldı. Sulandırılmış sperma
örneklerini içeren payetler +4 °C’de 2 saat süreyle ekilibre edildikten sonra sıvı azot
buharında (-120 °C) 15 dakika süreyle donduruldu ve sıvı azot (-196 °C) içinde saklandı. Tüm gruplardaki payetler 37 °C’de 30 saniye süreyle çözdürüldü. Her bir koça
ait çözdürülmüş sperma örneklerinde spermatozoa motilitesi (%), canlı spermatozoa
(%) ve anormal spermatozoa (%) yönünden değerlendirildi.
Yağsız süt tozu sulandırıcısı ile dondurulan
sperma örneklerinde çözüm sonu daha yüksek motilite elde edilirken, en düşük
motilite oranı Bioxcell sulandırıcısından elde edilmiştir. Sonuç olarak, sunulan çalışmada elde edilen in vitro sperma kalitesi
bulgularına göre yağsız süt tozu sulandırıcısının diğer sulandırıcılardan daha
üstün olduğu ve koçlar arası bireysel farkı azalttığı
bulunmuştur.

Anahtar Kelimeler

Bireysellik, Koç, Sperma, Sulandırıcılar.about:blankr: Bireysellik, Sulandırıcılar

Response to Different Semen Extenders of Individual Ram Semen

Öz

This
study was conducted to evaluate individual differences in freezing ram sperm
and to determine the extenders and individual sperm interaction. The ejaculates were collected from 5 Karayaka rams by artificial
vagina twice a week during the breeding season. The each ejaculate was split
into 4 equal aliquots and diluted with skimmed milk, Tris, Andromed and
Bioxcell extenders. Straws contained semen was equilibrated at +4 °C for 2 h and than frozen in vapor of (15 min at -120 °C) liquid nitrogen and stored in liquid nitrogen. Frozen semen was
thawed in a water bath at 37 °C for 30 seconds. In thawed semen, percentages of spermatozoa
motility, viability, and abnormal spermatozoa, for each ejaculate were
determined. The post-thaw sperm motility was significantly higher in skimmed
milk extender post-thaw, while lowest motility rate was obtained from Bioxcell
diluent. Consequently, it was found that skimmed milk extender was better than
the other extenders and decreased individual differences between rams.

Anahtar Kelimeler

Extenders, Individuality, Ram, Semen

Kaynakça

• Barbas JP, Mascarenhas RD, 2009: Cryopreservation of domestic animal sperm cells. Cell Tissue Bank, 10, 49-62.
• Dorji P, Pattarajinda V, Vongprolub V, 2014: Cryopreservation of semen of mithun and siri bulls. Bangl J Vet Med, 12, 147-153.
• Fraser L, Chandra SP, Strzeżek J, 2008: Identification of amplified fragment length polymorphism markers associated with freezability of boar semen–a preliminary study. Med Weter, 64, 646-649.
• Gil J, Rodriguez-Irazoqui M, Lundeheim N, Soderquist L, Rodriguez-Martinez H, 2003: Fertility of ram semen frozen in Bioxcell and used for cervical artificial insemination. Theriogenology, 59, 1157-1170.
• Gündoğan M, 2009: Short term preservation of ram semen with different extenders. Kafkas Univ Vet Fak Derg, 15, 429- 435.
• Holt WV, 2000: Fundamental aspects on sperm cryobiology: the importance of species and individual differences. Theriogenology, 53, 47-58.
• Kulaksiz R, Daşkın A, 2007: Teke spermasının kısa ve uzun surely saklanması. Vet Hek Dern Derg, 78, 4, 51-56.
• Kulaksiz R, Çebi̇ Ç, Akçay E, 2012: The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. Turk J Vet Anim Sci, 36, 177-182.
• Lopez-Saez A, Ortiz N, Gallego L, Gadre JJ, 2000: Liquid storage (5 °C) of ram semen in different diluents. Arch Androl, 44, 155-164.
• Medrano A, Watson PF, Holt WV, 2002: Importance of cooling rate and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Reproduction, 123, 315-322.
• Lymberopoulus AG, Khalifa TA, 2010: Sperm chromatin stability during in vitro manipulation of beef bull semen. Reprod Domest Anim, 45, 2, 307-314.
• O’Hara L, Hanrahan JP, Richardson L, Donovan A, Fair S, Evans AC, Lonergan P: 2010: Effect of storage duration, storage temperature, and diluent on the viability and fertility of fresh ram sperm. Theriogenology, 73, 4, 541-549.
• Paulenz H, Kommisrud E, Hofmo PO, 2000: Effect of long-term storage at different temperatures on the qual- ity of liquid boar semen. Reprod Domest Anim, 35, 83-87.
• Plante G, Luisgnan MF, Lafleur M, Manjunath P, 2015: Intraction of milk proteins and Binder of Sperm (BSP) Proteins from boar, stallion and ram semen. Reprod Biol and Endocrin, 13, 1-13.
• Roca J, Hernandez M, Carvajal G, Vazquez JM, Martinez EA, 2006: Factors influencing boar sperm cryosurvival. J Anim Sci, 84, 2692-2699.
• Ros-Santaella JL, Dominguez-Rebolledo AE, Grade JJ, 2014: Sperm flagellum volume vetermines freezability in Red Deer spermatozoa. PLOS ONE, 9, 11, 1-11.
• Smith JF, Asher GW, Briggs RM, Morrow CJ, Murray GR, Oliver JE, Parr J, Veldhuizen FA, Upreti GC, 1993: Effect of diluent and storage time on pregnancy rate in ewes after intrauterine insemination. Proceedings of the New Zealand Society of Animal Production, 53, 295-298.
• Salamon S, Maxwell WM (2000): Storage of ram semen. Anim Reprod Sci, 62, 77-111.
• Thurston LM, Siggins K, Mileham AJ, Watson PF, Holt WV, 2002: Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation. Biol Reprod, 66, 545-554.
• Watson PF, 1995: Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod Fertil Dev, 7, 871-891.