Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi

Abstract

Naringenin, insan sağlığı üzerinde biyoaktif bir etkiye sahip olup, greyfurtta baskın bulunan doğal bir flavonondur. Bu çalışmada ratlarda sisplatin ile oluşturulan nefrotoksisite üzerine naringenin’in böbrek dokusundaki bazı biyokimyasal parametreler üzerine etkileri araştırıldı. Bu çalışmada, 35 adet 2 aylık wistar albino ratlar kullanıldı. Ratlar rastgele her grupta 7 rat olacak şekilde 5 gruba ayrıldı. 1.grup (Kontrol) %1’lik DMSO i.p, 2.grup  (Cis), tek doz sisplatin., 7 mg/kg / i.p, 3.grup (NG₂₀) naringenin, 20 mg/kg/10 gün /i.p, 4.grup, (Cis+NG₂₀ ) tekdoz sisplatin 7 mg/kg/ i.p + 20 mg/kg/10 gün./i.p naringenin, 5.grup (Cis+NG₄₀) tek doz sisplatin 7 mg/kg/ i.p + 40 mg/kg/10 gün./i.p naringenin  on gün boyunca uygulandı. Çalışma sonunda ratlardan alınan böbrek dokusundan biyokimyasal analizler yapıldı. Sisplatin grubunda böbrek TOS, OSI, MDA, AOPP, 8-OHdG ve NRF-2 düzeyleri kontrol grubuna göre artarken (P<0.05), böbrek TAS ve GSH (P<0.05) düzeyleri anlamlı olarak azaldı. Sisplatinin ratlarda oluşturduğu nefrotoksisiteyi, naringenin’in anlamlı olarak azalttığından dolayı, sisplatin'e bağlı nefrotoksisitenin naringenin ile kontrol edilebileceği sonucuna varılmıştır.

Keywords

• Naringenin,DNA hasarı,NRF-2,TAS,TOS

Investigation of the Protective Effect of Naringenin on the Cisplatin-Induced Nephrotoxicity in Rats

Abstract

Naringenin which have a bioactive effect on human health is a natural flavonone predominantly found in grapefruit. In this study, the effects of naringenin on some biochemical parameters in renal tissue in cisplatin-induced nephrotoxicity case were investigated in rats. In this study 35 Wistar albino rats, aged 2 months were used. Rats were randomly divided into 5 groups each consisted of 7 rats,; 1.group (control) 1 % DMSO i.p, 2.group  (Cis), one dose cisplatin., 7 mg/kg/ i.p, 3.group (NG20) naringenin, 20 mg/kg/ day /i.p, 4.group, (Cis+NG₂₀ ) one dose cisplatin 7 mg/kg/ i.p + 20 mg/kg/day./i.p naringenin, 5.group (Cis+NG₄₀) one dose cisplatin 7 mg/kg/ i.p + 40 mg/kg/day./i.p naringenin were administered for ten days. At the end of the study biochemical analyses were made on samples of kidney tissues. Kidney tissue levels of TOS, OSI, MDA, AOPP, 8-OHdG ve NRF-2 were increased (P<0.05) and TAS and GSH levels (P<0.05) were decreased significantly in cisplatin group (2. group) compared with control group. As naringenin significantly reduced nephrotoxicity in cisplatin-induced rats and nephrotoxicity could be controlled with naringenin.

Keywords

• Naringenin,DNA damage,NRF-2,TAS,TOS

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