Yüksek Yağlı Diyet ile Beslenen Ratlarda Mate (Ilex paraguariensis) Çayının Obeziteyi Önleyici Etkisinin Histopatolojik ve Biyokimyasal Olarak Araştırılması

Abstract

Bu çalışmada yüksek yağlı diyetle beslenen ratlarda, Mate yaprağı (Ilex paraguariensis) çayının obezite oluşumu üzerine etkisi histopatolojik ve biyokimyasal olarak araştırıldı. Bu amaçla 32 adet Wistar albino rat; Kontrol (K) (n: 8), Yüksek Yağlı Diyet (YYD) (n: 8), YYD+Mate (YYD+M) (n: 8) ve Mate (M) (n: 8) olmak üzere 4 gruba ayrıldı. Kontrol grubu ratlar standart pelet yemle beslendi. YYD ve YYD+M grubu ratlara, 300 g/kg oranında tereyağı eritilip yeme katılarak enerji değeri yüksek (4165 kcal/kg) hale getirilen pelet yem verildi. YYD+M ve M grubu ratlar ise 3 gr/kg vücut ağırlığı olacak şekilde günlük hazırlanan Mate yaprağı (Ilex paraguariensis) çayı orogastrik sonda ile verildi. Çalışma süresince ratların canlı ağırlık artışları izlendi. Histopatolojik olarak karaciğerlerden alınan doku örneklerinde YYD grubunda yağlanma, hidropik dejenerasyon ve bazı hepatositlerde koagulasyon nekrozları görülürken, YYD+M grubunda tek tük yağlanma görüldü. Biyokimyasal analizlerde ALT, AST, ALP, LDH, GGT, kolesterol, trigliserit, HDL ve açlık kan glukoz düzeyleri YYD grubunda kontrol grubuna göre belirgin düzeyde yüksek, YYD+M grubunda ise kontrol grubuna yakın olduğu tespit edildi. Sonuç olarak, YYD ile birlikte verilen Mate yaprağı (Ilex paraguariensis) çayının ratlarda obezite oluşumunun önüne geçebileceği kanaatine varıldı.

Keywords

• Antiobesite etki,Mate,Yüksek yağlı diyet

Histopathological and Biochemical Investigation of the Effects of Mate (Ilex paraguariensis) Tea on Antiobesity Effect in Rats Fed with High Fat Diet

Abstract

The aim of this study was to investigate the preventative effect of Mate tea (Ilex paraguariensis) tea on obesity development in rats fed with high fat diet by histopathological and biochemical methods. In this study, 32 Wistar albino rats were divided into four groups as Control Group (C) (n:8), High Fat Diet Group (YYD) (n: 8), High Fat Diet+Mate Group (YYD+M) (n: 8) and Mate Group (M) (n: 8). YYD and YYD+M group rats received YYD (4165 kcal/kg) containing fat (300 g/kg) for 8 weeks. The daily preparation of Mate leaf (Ilex paraguariensis) tea at the dose of 3 g/kg body weight in rats of group YYD+M and M group was given by orogastric catheter. During the study, weight gain of the rats was monitored. Hepatoprotective effect by Ilex paraguariensis tea was further supported by the almost normal histology in YYD+M group as compared to the degenerative changes such as fattenings, hydropic degeneration and coagulation necrosis of some hepatocyte in the YYD treated rats. Decreased levels of blood glucose AST, ALT, ALP, LDH, GGT, cholesterol, triglycerides, HDL cholesterol and glocose were detected in YYD+M group. These findings suggested that simultaneous treatment of Ilex paraguariensis attenuated hepatic fat accumulation in YYD fed rats.

Keywords

• Antiobesity effect,High-fat diet,Ilex paraguariensis

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