Determination of Genetic Polymorphisms of Leptin, Ghreline and Insulin Like Growth Factor-1 (IGF-1) Genes in Beef Cattle Raised in Turkey

Abstract

This study was performed to determine polymorphisms in leptin, ghrelin and insulin like growth factor-1 (IGF-1) genes and to identify genotype and alleles frequencies in beef cattles raised in Sanliurfa province of Turkey. A total of 405 male beef cattles, which were raised in Harranova Live stock and Agricultural Enterprise in Şanlıurfa and selected by phenotypic evaluation, were included in to the study. The animal material consisted of Hereford (n=112), Angus (n=145), Charolais (n=54), Black Hereford (n=36), Brahman (n=24)  and Limousin (n=34) cattles. After DNA isolation from meat samples, leptin, ghrelin and insulin like growth factor-1 (IGF-1) gene polymorphisms were determined by using PCR-RFLP method. All three possible genotypes of BB, AB and AA were observed in IGF-1/SnaBI gene AB and AA genotypes had the highest and the lowest frequencies, respectively. The frequency of B allele (0.600) was higher than that of A allele (0.400).  With respect to PstI polymorphism at the leptin gene, three possible genotypes were also observed. In this locus; genotypes having the highest and the lowest frequency were CT and TT respectively and the frequency of C allele was 0.571 while frequency of T was 0.429. At the BfaI polymorphic site of Ghrelin gene in AA and AG genotypes were observed while GG genotype was not observed. Accordingly, the frequency of the A allele was found to be high (0.938) and the frequency of the G allele was found to be quite low (0.062). As a result, it was determined that the examined beef cattle populations were in the Hardy-Weinberg equilibrium in terms for IGF-1 / SnaBI and Ghrelin / BfaI polymorphisms but not for Leptin/PstI polymorphism. 

Keywords

• Genetic polymorphisms,Beef cattle,Leptin,Ghreline,IGF-1

Türkiye’de Yetiştirilen Et Irkı Kültür Sığırlarında Leptin, Ghrelin ve İnsülin Benzeri Büyüme Faktörü -1 (IGF-1) Gen Polimorfizmlerinin Belirlenmesi

Abstract

Bu çalışma, Türkiye’de yetiştirilen et ırkı kültür sığırlarında leptin, ghrelin ve insülin benzeri büyüme faktörü -1 (IGF-1) geni polimorfizmlerinin belirlenmesi, polimorfizmler yönünden genotip ve allel sıklıklarının tespit edilmesi amacıyla yapılmıştır. Çalışmanın materyalini Şanlıurfa Harranova Besi ve Tarım İşletmesi’nde bulunan ve fenotipik değerlendirmeyle seçilmiş Hereford (n=112), Angus (n=145), Şarole (n=54), 36 Siyah Hereford (n=36), Brahman (n=24) ve Limousin (n=34) ırkı toplam 405 baş erkek hayvan oluşturmuştur. Hayvanların et örneklerinden DNA izole edildikten sonra leptin, ghrelin ve IGF-1 gen polimorfizmleri PCR-RFLP yöntemiyle belirlenmiştir. Sonuç olarak incelenen besi sığırı sürüsünde IGF-1/SnaBI polimorfizmi yönünden BB, AB ve AA genotiplerinin her üçü de gözlemlenmiştir. IGF-1 lokusunda sıklığı en yüksek genotip AB, en düşük genotip ise AA şeklinde bulunmuş olup B allelinin sıklığı (0.600) A alleline göre (0.400) yüksek olduğu gözlenmiştir. Leptin/PstI polimorfizmi yönünden bakıldığında da yine olası üç genotip gözlenmiştir. Bu lokusta sıklığı en yüksek genotip CT, en düşük genotip ise TT olarak bulunmuş, C allelinin sıklığı 0.571 bulunurken T allelinin sıklığı 0.429 olarak tespit edilmiştir. Ghrelin/BfaI polimorfizmi yönünden ise incelenen materyalde AA ve AG genotipleri gözlenirken GG genotipi gözlenmemiştir. Buna bağlı olarak A allelinin sıklığı yüksek bulunurken (0.938), G allel sıklığı ise oldukça düşük (0.062) bulunmuştur. Sonuç olarak incelenen besi sığırı populasyonlarının IGF-1/SnaBI ve Ghrelin/BfaI polimorfizmleri yönünden Hardy-Weinberg dengesinde, Leptin/PstI polimorfizmi yönünden ise dengede olmadığı belirlenmiştir.

Keywords

• Genetik polimorfizm,Besi sığırları,Leptin,Ghrelin,IGF-1

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