Streptozotosin Kaynaklı Diyabetik Sıçanlarda Diplotaenia Turcica Kök Ekstraktının Sperm Parametreleri ve Üreme Hormonları Üzerine Etkileri

Abstract

Bu çalışma, Streptozotosin (STZ) kaynaklı diyabetik sıçanlarda Diplotaenia turcica kök ekstraktının sperm parametreleri ve üreme hormonları üzerindeki etkilerini belirlemek amacıyla yapıldı. Çalışma eşit sayıda 6 gruba ayrılan toplam 42 erkek rat üzerinde yapıldı. Grup 1'e intraperitoneal olarak tek doz serum fizyolojik uygulandı. Grup 2'ye intraperitonal yolla STZ 45 mg/kg uygulandı. Grup 3’e 100 mg/kg Diplotaenia turcica kök ekstraktı 28 gün boyunca gastrik gavajla uygulandı. Grup 4’e 200 mg/kg Diplotaenia turcica kök ekstraktı 28 gün boyunca gastrik gavajla uygulandı. STZ 45 mg/kg, Grup 5'e intraperitonal olarak uygulandı. Ek olarak Diplotaenia turcica kök ekstraktı 100 mg/kg, 28 gün boyunca gastrik gavaj yoluyla uygulandı. STZ 45 mg/kg, Grup 6'ya intraperitonal olarak uygulandı. Ek olarak Diplotaenia turcica kök ekstraktı 200 mg/kg, 28 gün boyunca gastrik gavaj yoluyla uygulandı. Üreme hormonları ve sperm parametreleri analiz edildi. Grup 2'de sperm motilitesi ve yoğunluğu ile üreme hormonu değerlerinin Grup 1'e göre anlamlı derecede düşük olduğu ve anormal sperm oranının Grup 1'e göre anlamlı derecede yüksek olduğu belirlendi. Grup 3 ve 4’ün testosteron düzeyleri, Grup 2, 5 ve 6’ya göre oldukça yüksekti. Diyabetli gruba ilave olarak verilen Diplotaenia turcica kök ekstraktının 100 mg/kg’lık dozunun sperm parametrelerinde ve testosteron hormonunda bir iyileştirme oluşturduğu, ancak 200 mg/kg’lık dozunun aynı etkiyi oluşturmadığı tespit edildi. Sonuç olarak, Diplotaenia turcica kök ekstraktının 100 mg/kg dozunu, diyabetli hastalarda önerebiliriz.

Keywords

• Diplotaenia turcica kök ekstraktı
• Diyabet
• Erkek Sıçan
• Sperm Parametreleri
• Üreme hormonları

The Effects of Diplotaenia Turcica Root Extract on Sperm Parameters and Reproductive Hormones in Streptozotocin Induced Diabetic Rats

Abstract

This study was conducted to determine the effects of Diplotaenia turcica root extract on sperm parameters and reproductive hormones in streptozotocin (STZ) induced diabetic rats. The study was performed on a total of 42 male rats divided into 6 groups of equal numbers. Single-dose physiological saline was administrated intraperitoneally to Group 1. STZ 45 mg/kg was administered intraperitoneally to Group 2. Diplotaenia turcica root extract 100 mg/kg was administrated through gastric gavage for 28 days to Group 3. Diplotaenia turcica root extract 200 mg/kg was administrated through gastric gavage for 28 days to Group 4. STZ 45 mg/kg administrated intraperitoneally to Group 5. In addition, Diplotaenia turcica root extract 100 mg/kg was administrated through gastric gavage for 28 days. STZ 45 mg/kg administrated intraperitoneally to Group 6. Also, Diplotaenia turcica root extract 200 mg/kg was administrated through gastric gavage for 28 days. Reproductive hormones and sperm parameters were analyzed. In Group 2, it was determined that sperm motility and density and reproductive hormone values were significantly lower when compared to group 1, and the abnormal sperm rate was significantly higher when compared to Group 1. Testosterone levels in Groups 3 and 4 were significantly higher than Groups 2, 5, and 6. It was determined that the 100 mg/kg dose of Diplotaenia turcica root extract, which is given also to the diabetic group, creates an improvement in sperm parameters and the hormone testosterone, but the 200 mg/kg dose does not have the same effect. As a result, we can recommend the 100 mg/kg dose of Diplotaenia turcica root extract in patients with diabetes.

Keywords

• Diabetes
• Diplotaenia turcica root extract
• Male rat
• Reproductive hormones
• Sperm parameters

References

1. Aksu EH, Akman O, Özkaraca M, Ömür AD, Uçar Ö, 2015: Effect of Maclura pomifera extract against cisplatin‐induced damage in reproductive system of male rats. Kafkas Univ Vet Fak Derg, 21(3), 397-403.
2. Artimani T, Amiri I, Soleimani Asl S, Saidijam M et al., 2018: Amelioration of diabetesinduced testicular and sperm damage in rats by cerium oxide nanoparticle treatment. Andrologia 50(9), e13089.
3. Bal R, Türk G, Tuzcu M, Yilmaz O, Ozercan I et al. 2011: Protective effects of nanostructures of hydrate C(60)fullerene on reproductive function in streptozotocin-diabetic male rats. Toxicology, 282(3), 69-81.
4. Ballester J, Muñoz MC, Domínguez J, Rigau T, Guinovart JJ et al., 2004: Insulin-dependent diabetes affects testicular function by FSH and LH-linked mechanisms. J Androl, 25(5), 706-719.
5. Baytop T, 1984: Treatment with Plants in Turkey. İstanbul, Istanbul University Publications. 3255. Dawson EB, Harris WA, Teter MC, Powell LC, 1992: Effect of ascorbic acid supplementation on the sperm quality of smokers. Fertil Steril, 58(5): 1034-1039. Farkhad NK, Farokhi F, Tukmacki A, 2012: Hydro-alcoholic extract of the root of Prangos ferulacea (L.) Lindl can improve serum glucose and lipids in alloxan-induced diabetic rats. Avicenna J Phytomed, 2(4), 179-187.
6. Fedail JS, Ahmed AA, Musa HH, Ismail E, Sifaldin AZ et al. 2016: Gum arabic improves semen quality and oxidative stress capacity in alloxan induced diabetes rats. APJR, 5(5), 434-441. Isidro ML, 2011: Sexual dysfunction in men with type 2 diabetes. Postgrad Med J, 88(1037), 152-159.
7. Kaval I, Behçet L, Cakilcioglu U, 2014: Ethnobotanical study on medicinal plants in Geçitli and its surrounding (Hakkari-Turkey). J Ethnopharmacol, 55(1), 171-184.
8. Kim KS, Kang SH, Kim MJ, Kim SK, Kim YL et al. 2014: Low serum testosterone concentrations in hospitalized men with poorly controlled type 2 diabetes. Endocrinol Metab (Seoul), 29(4): 574-578. Kumar V, Jain P, Rathore K, Ahmed Z, 2016: Biological evaluation of Pupalia lappacea for antidiabetic, antiadipogenic, and hypolipidemic activity both in vitro and in vivo. Scientifica, 2016(9), 1-10.

9. Lert-Amornpat T, Maketon C, Fungfuang W, 2017: Effect of Kaempferia parviflora on sexual performance in streptozotocin-induced diabetic malerats. Andrologia, 49, e12770.
10. Nelli GB, K AS, Kilari EK, 2013: Antidiabetic effect of α-mangostin and its protective role in sexual dysfunction of streptozotocin induced diabetic male rats. Syst Biol Reprod Med, 59(6), 319-328.
11. O’Hara L, Smith LB, 2015: Androgen receptor roles in spermatogenesis and infertility. Best practice & research. Best Pract Res Clin Endocrinol Metab, 29(4), 595-605.
12. Özdek U, Başbuğan Y, Yıldırım S, Boğa M, Fırat M et al., 2018: Activity, acute and Sub-acute toxicity and safety assesment of the hydroalcholic root extract of Diplotaenia turcica. IJAR, 52(12), 1688-1694.
13. Özdek U, Değer Y, 2020: The effect of Diplotaenia turcica root extract in streptozotocin-induced diabetic rats. Turk J Bıochem, 45(4).
14. Patil SA, Sujaya M, Patil SB, 2014: Aphrodisiac and phytochemical studies of Cocculus hirsutus extracts in albino rats. APJR, 3(1), 23-29.
15. Pimenov M, Kljuykov E, Degtjareva G, 2011: Survey of the genus Diplotaenia (Umbelliferae), with description of two new species from Turkey. Willdenowia, 41(1), 67-74.
16. Rashid K, Sil PC, 2015: Curcumin ameliorates testicular damage in diabetic rats by suppressing cellular stress-mediated mitochondria and endoplasmic reticulum-dependent apoptotic death. Biochim Biophys Acta, 1852(1), 70-82. Soliman GA, Saeedan AS, Abdel-Rahman RF, Ogaly HA, Abd-Elsalam RM et al., 2019: Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study. Saudi Pharm J, 27(3), 326-340.
17. Sonmez M, Turk G, Yuce A, 2005: The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology, 63, 2063-2072. Suresh S, Prakash S, 2012: Effect of Mucuna pruriens (Linn.) on sexual behavior and sperm parameters in streptozotocin-induced diabetic male rat. J Sex Med, 9, 3066-307. Turk G, Atessahin A, Sonmez M, Ceribasi AO, Yuce A, 2008: Improvement of cisplatin‐induced injuries to sperm quality, the oxidant– antioxidant system, and the histologic structure of the rat testis by ellagic acid. Fertil Steril, 89(5), 1474-1481.