CAN GANODERMA LUCIDUM BE AN ALTERNATIVE NUTRITIONAL SUPPLEMENT FOR ENHANCING SPERM MOTILITY RATE?

Yıl 2021, Cilt: 4 Sayı: 1, 47 - 61, 30.05.2021

Başak Büyük Tuba Demirci Neslihan Demir Hakan Türkön

https://doi.org/10.52538/iduhes.886093

Öz

Ganoderma lucidum (GL) is a widely used medicinal mushroom. The therapeutic effect of this fungus on many diseases has been proven by studies. The aim of this study is to assess the effects of low, moderate and high dose GL extract administration on the testis tissue, spermatogenic series cells and sperm motility in rats. 40 Wistar albino rats were randomly divided into 4 groups. Rats in group 1, 2, 3 and 4 were administered 2 ml physiologic serum, 500 mg/kg, 2500 mg/kg, 5000 mg/kg Ganoderma lucidum extract 1 time per day via gavage for 9 days, respectively. For evaluation of sperm motility and histopathological changes, epididymal sperm collection and testis harvesting were done. Blood samples were collected for biochemical analysis. When the Group 1 (control group) is compared with Groups 2, 3 and 4, the Johnsen score and sperm motility in these groups increased and this increase was statistically significant. In conclusion, low, moderate, and high doses of GL extract administered to rats were revealed to increase spermatogenesis, epididymal total sperm count and progressive motile sperm counts. However, it is detected that high doses cause minimal damage to the testis and as the increase in sperm parameters wasn’t significant, it’s concluded that doses for oral use above 2500 mg/kg should be avoided.

Anahtar Kelimeler

Ganoderma lucidum, Male fertility, Sperm motility, Testis histopathology

Destekleyen Kurum

Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

TSA-2018-2658

Teşekkür

This study was supported by Canakkale Onsekiz Mart University The Scientific Research Coordination Unit, Project number: TSA-2018-2658.

GANODERMA LUCIDUM SPERM MOTILITE ORANINI ARTTIRMAK İÇİN ALTERNATİF BİR BESLENME DESTEĞİ OLABİLİR Mİ?

Öz

Ganoderma lucidum (GL) yaygın olarak kullanılan tıbbi bir mantardır. Bu mantarın terapötik etkileri daha önceki birçok çalışmada kullanılmıştır. Bu çalışmanın amacı, düşük, orta ve yüksek doz uygulanan GL ekstresinin sıçanlarda testis dokusu, spermatogenic seri hücreleri ve sperm motilitesi üzerine etkisini araştırmaktır. Bu amaçla 40 adet Wistar albino cinsi erkek sıçan rastgele dört gruba ayrılmıştır. Grup 1’deki sıçanlara 2 ml serum fizyolojik, Grup 2, 3 ve 4’deki sıçanlara ise sırasıyla 500 mg/kg, 2500 mg/kg ve 5000 mg/kg Ganoderma lucidum ekstresi toplam dokuz gün ve günde bir kez gavaj yoluyla verilmiştir. Sperm motilitesi ve histopatolojik değişiklikleri değerlendirmek için epididimal sperm toplama işlemi yapıldı ve testis dokusundan örnek alındı. Kan örnekleri ise biyokimyasal değerlendirme için alındı. Grup 1 (Kontrol) grubu ile Grup 2, 3 ve 4 karşılaştırıldığında Johnsen skoru ve sperm motilitesi bu gruplarda artmış olarak bulundu ve bu artış istatistiksel olarak anlamlıydı. Sonuç olarak, uygulanan düşük, orta ve yüksek doz GL ekstresinin spermatogenezi, epididimal total sperm sayısını ve progresif motil sperm sayısını artırdığı ortaya konmuştur. Ancak, yüksek dozlar testis dokusunda minimal hasara neden olmuştur, ayrıca sperm parametrelerindeki yükselme anlamlı bulunmamıştır. Bu nedenle, 2500 mg/kg'ın üzerindeki oral kullanım dozlarından kaçınılması gerektiği sonucuna varılmıştır.

Anahtar Kelimeler

Ganoderma lucidum, erkek infertilitesi, sperm motilitesi, testis histopatolojisi

Proje Numarası

TSA-2018-2658

Kaynakça

• Barrett, K.E., Barman, S.M., Boitano, S., & Brooks, H.L. (2015). Ganong’s Review of Medical Physiology (Turkish Edition). İstanbul. Mc Graw Hill.p.420-421. ISBN: 978-605-335-091-0.
• Buswell, J.A., Benzie, F.F., Yuen, J., Galor, S.W. (2011). Herbal Medicine: Biomolecular and Clinical Aspects (Chapter 9) (2nd edition). Boca Raton (FL): CRC Press/Taylor & Francis.. p.232-248
• Cör, D., Knez, Ž., Knez Hrnčič, M. (2018). Antitumour, Antimicrobial, Antioxidant and Antiacetylcholinesterase Effect of Ganoderma Lucidum Terpenoids and Polysaccharides: A Review. Molecules, 23(3), 649.
• Donmez, D.B., Yılmaz, G. (2014). Effects of Ganoderma Lucidum (Red Reishi mushroom) on the Genital System in Female Rats. Merit Res J Med Sci, 2(7), 141-145.
• Engelbrecht, K., Volk, T. (2005). Ganoderma lucidum, Reishi or Ling Zhi, a fungus used in oriental medicine. http://botit.botany.wisc.edu/toms_fungi/mar2005.html
• Fujita, R., Liu, J., Shimizu, K., Konishi, F., Noda, K., Kumamoto, S., Ueda, C., Tajiri, H., Kanedo, S., Suimi, Y., Kondo, R. (2005). Anti-androgenic activities of Ganoderma lucidum. J Ethnopharmacol, 102(1), 107-112.
• Johnsen, S.G. (1970) Testicular biopsy score count–a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Horm Res Paediatr, 1(1), 2-25.
• Lee, J.M., Kwon, H., Jeong, H., Lee, J.W., Lee, S.Y., Baek, S.J., Surh, Y. (2001). Inhibition of lipid peroxidation and oxidative DNA damage by Ganoderma lucidum. Phytother Res, 15(3), 245-249.
• Lin, Z.B., Zhang, H.N. (2004). Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its possible mechanisms. Acta Pharmacol Sin, 25,1387-1395.
• Liu, J., Tamura, S., Kurashiki, K., Shimizu, K., Noda, K., Konishi, F., Kumamoto, S., Kondo, R. (2009). Anti‐Androgen Effects of Extracts and Compounds from Ganoderma lucidum. Chem Biodivers, 6(2), 231-243.
• Ma, H.T., Hsieh, J.F., Chen, S.T. (2015). Anti-diabetic effects of Ganoderma lucidum. Phytochemistry, 114, 109-113.
• Moskvin, S.V., Apolikhin, O.I. (2018). Effectiveness of low level laser therapy for treating male infertility. BioMed, 8(2).
• Naghdi, M., Maghbool, M., Seifalah-Zade, M., Mahaldashtian, M., Makoolati, Z., Kouhpayeh, S.A., Ghasemi, A., Fereydouni, N. (2006). Effects of common fig (Ficus carica) leaf extracts on sperm parameters and testis of mice intoxicated with formaldehyde. Evid Based Complement Alternat Med, 16.
• Özevren, H., İrtegün, S., Deveci, E., Aşır, F., Pektanç, G., Deveci, Ş. (2017). Ganoderma Lucidum Protects Rat Brain Tissue Against Trauma-Induced Oxidative Stress. Korean J Neurotrauma, 13(2),76-84.
• Rhoades, R.A., Bell, D.R. (2017). Medical Physiology, Principles for Clinical Medicine (Turkish Edition). Lippincott Williams & Wilkins. p. 686-692. ISBN: 978-605-9528-03-0.
• Sengupta, P., Borges, Jr E., Dutta, S., Krajewska-Kulak, E. (2018). Decline in sperm count in European men during the past 50 years. Hum Exp Toxicol, 37(3), 247-255.
• Shevelev,. O.B., Seryapina, A.A., Zavjalov, E.L., Gerlinskaya, L.A., Goryachkovskaya, T.N., Slynko, N.M., Kuibida, L. V., Peltek, S. E., Markel, A. L., Moshkin, M. P. (2018). Hypotensive and neurometabolic effects of intragastric Reishi (Ganoderma lucidum) administration in hypertensive ISIAH rat strain. Phytomedicine, 41, 1-6.
• Shiao, M.S. (2003). Natural products of the medicinal fungus Ganoderma lucidum: occurrence, biological activities, and pharmacological functions. Chem Rec, 3(3), 172-180.
• Sliva, D. (2003). Ganoderma lucidum (Reishi) in cancer treatment. Integr Cancer Ther, 2(4), 358-364.
• Sukhn, C., Awwad, J., Ghantous, A., Zaatari, G. (2018). Associations of semen quality with non-essential heavy metals in blood and seminal fluid: data from the Environment and Male Infertility (EMI) study in Lebanon. J Assist Reprod Genet, 1-11.
• Sun, J., He, H., Xie, B.J. (2004). Novel antioxidant peptides from fermented mushroom Ganoderma lucidum. J Agric Food Chem, 52(21), 6646-6652. Tang, W., Liu, J.W., Zhao, W.M., Wei, D.Z., Zhong, J.J. (2006). Ganoderic acid T from Ganoderma lucidum mycelia induces mitochondria mediated apoptosis in lung cancer cells. Life Sci. 80(3), 205-211.
• Wang, B.X., Wang, S.Q., Qin, W.B., Wang, S.X., Ma, X.A., Zhang, T. (2006). Effects of ganoderma lucidum spores on cytochrome C and mitochondrial calcium in the testis of NIDDM rats. Zhonghua nan ke xue, 12(12), 1072-1075.
• Wang, S.Q., Qin, W.B., Kang, Y.M., Ma, X.R., Liu, L., Liu, J.X., Zhang, T., Zhang, B., Liang, Y. F., Wang, F. F. (2008). Intervention effect of ganoderma lucidum spores on the changes of XOD, MPO and SDH in the testis tissue of NIDDM rats. Zhonghua nan ke xue, 14(9), 792-795.
• Wang, J., Cao, B., Zhao, H., Feng, J. (2017). Emerging roles of Ganoderma Lucidum in anti-aging. Aging Dis, 8(6), 691.
• Yuen, J.W., Gohel, M.D.I. (2005). Anticancer effects of Ganoderma lucidum: a review of scientific evidence. Nutr Cancer, 53(1), 11-17.
• Zhong, L.L., Wang, S.Q., Zhang, W.J. (2006). Effect of ganoderma lucidum on the mechanism of ROS damage of NIDDM rat testis. Heilongjiang Med Pharm, 4, 000.