Kadmiyuma (CD) maruz kalan dişi sıçanlarda Lactobacillus plantarum (LP) uygulamasının bazı kan parametreleri ve ANAE-pozitif lenfosit oranları üzerine kısmi koruyucu etkileri

Yıl 2023, Cilt: 6 Sayı: 1, 160 - 170, 26.05.2023

İhsan Kısadere , Hakan Tavşanlı , Mehmet Faruk Aydın , Özkan Demirbaş

https://doi.org/10.52538/iduhes.1251431

Öz

Bu çalışmada, kronik olarak kadmiyuma (CD) maruz kalan dişi sıçanlarda oral Lactobacillus plantarum (LP) uygulamasının bazı kan parametreleri ile ANAE-pozitif lenfosit oranları üzerindeki etkilerini araştırmayı amaçladık. Bu araştırmanın yapılabilmesi için ratlar kontrol (K, n = 8), kadmiyum (CD, n = 8), LP (n = 8) ve CDLP (n = 8) olmak üzere dört eşit gruba ayrıldı. CD (2.04 mg/mL) oral olarak CD ve CDLP gruplarına 28 gün uygulandı. Ayrıca LP ve CDLP gruplarına aynı anda aktif-canlı LP (yaklaşık 108 - 109 cfu/mL) oral olarak uygulandı. C grubu hayvanlara çalışma süresince sadece taze su ve standart rat yemi verildi. Uygulamaların ardından hayvanlar dekapite edilerek bazı kan parametreleri (WBC ve farklı alt tipleri, RBC, HCT, MCV, MCH, MCHC ve PLT) ve ayrıca ANAE-pozitif lenfosit oranlarının değerlendirilmesi için kan örnekleri alındı. WBC sayıları diğer deney gruplarına göre CD grubunda en yüksek olarak belirlendi (p < 0,05). Öte yandan, LP uygulaması CDLP grubu hayvanlarda WBC sayılarında CD grubuna göre önemli bir iyileşmeye neden oldu (p < 0,05). NEU sayıları en yüksek CD grubunda saptanmakla birlikte, CDLP grubunda oral LP uygulamasına bağlı olarak bu sayılarda düşüş gözlemlendi (p < 0,05). Çalışmada CD uygulamasına bağlı olarak artan PLT değerleri, CDLP grubunda CD grubuna oranla LP diyeti kullanılarak düzeltildi (p < 0,05). Sonuç olarak LP uygulaması, dişi sıçanların kan parametrelerini CD'nin zararlı etkilerinden kısmen korumuştur.

Anahtar Kelimeler

Lactobacillus plantarum, kadmiyum, dişi rat, hematoloji, ANAE pozitif

PARTIAL PROTECTIVE EFFECTS OF L. PLANTARUM ADMINISTRATION ON SOME BLOOD PARAMETERS AND ANAE-POSITIVE LYMPHOCYTE RATIOS IN FEMALE RATS EXPOSED TO CADMIUM (Cd)

Öz

We aimed to research the influences of oral L.plantarum administration on some blood parameters and alpha-naphthyl acetate esterase (ANAE)-positive lymphocyte ratios in female rats exposed chronically to Cd. In order to perform this investigation, rats were separated into four equal groups as control (C, n = 8), cadmium (Cd, n = 8), L.plantarum (n = 8), and Cd+ L.plantarum (n = 8). Cd (2.04 mg / mL) was orally applicated to Cd and Cd+ L.plantarum groups for 28 days. In addition, active-live L.plantarum (approximately 108 - 109 cfu / mL) was applicated orally to L.plantarum and Cd+ L.plantarum groups at the same time. C group animals received only fresh water and standard rat food during the study. After the applications, animals were decapitated and blood samples were collected for evaluating of some blood parameters (WBC and its different sub-types, RBC, HCT, MCV, MCH, MCHC, and PLT) and also ANAE-positive lymphocyte ratios. WBC counts were determined the highest in the Cd group compared to other experimental groups (p < 0.05). On the other hand, LP administration caused a significant amelioration in WBC counts of Cd+ L.plantarum group animals when compared to Cd group (p < 0.05). Although NEU counts were detected the highest in the Cd group, these counts decreased due to oral L.plantarum administration in Cd+ L.plantarum group (p < 0.05). Increased PLT values due to CD administration were fixed by using LP diet in the Cd+ L.plantarum group compared to the Cd group in the study (p < 0.05). In conclusion, L.plantarum partially affects female rats’ blood parameters from the harmful effects of Cd.

Anahtar Kelimeler

Lactobacillus plantarum, kadmiyum, dişi rat, hematoloji, ANAE pozitif

Kaynakça

• Andjelkovic M, Djordjevic AB, Antonijevic E, Antonijevic B. (2019). Toxic effects of acute cadmium and lead exposure in rat blood, liver, and kidney. Int. J. Environ. Res. Pub. Health. 16: 274. Banwo K, Alonge Z, Sanni AI. (2021). Binding capacities and antioxidant activities of Lactobacillus plantarum and Pichia kudriavzevii against cadmium and lead toxicities. Biol. Trace. Elem. Res. 199(2): 779-791. https://doi.org/10.1007/s12011-020-02164-1.
• Banwo K, Alonge Z, Sanni AI. (2021). Binding capacities and antioxidant activities of Lactobacillus plantarum and Pichia kudriavzevii against cadmium and lead toxicities. Biol. Trace. Elem. Res. 199(2): 779-791. https://doi.org/10.1007/s12011-020-02164-1.
• Bernard A. (2008). Cadmium and its adverse effects on human health. Indian. J. Med. Res. 128 (4): 557-564. Bora S, Lakshman M, Madhuri D, Kalakumar B, Udayakumar B. (2022). Protective effects of Lactobacillus sporogenes against As-induced hematological alterations in male albino Wistar rats. Biol. Trace. Elem. 200: 4744-4749.
• Donmez HH, Sur E. (2008). Hematology and Enzyme Histochemistry of the Peripheral Blood Leucocytes in Rock Partridges (Alectoris graeca). Poultry. Sci. 87:56-60 . doi:10.3382/ps.2007-00328
• Dönmez HH, Dönmez N, Kisadere I, Undag I. (2019). Protective effect of quercetin on some hematological parameters in rats exposed to cadmium. Biotech. Histochem. 94 (5): 381-386. https://doi.10.1080/10520295.2019.1574027.
• Dönmez N, Dönmez HH, Kisadere I, Kadiralieva N. (2016). Some hematological values and alpha-naphthyl acetate esterase (ANAE)-positive lymphocyte ratios in Jaydara sheep. J. Advanced. Vet. Animal. Res. 3 (1): 8-12.
• Fazeli H, Moshtaghian J, Mirlohi M, Shirzad M. (2010). Reduction in serum lipid parameters by incorporation of a native strain of Lactobacillus Plantarum A7 in mice. J. Diabetes. Metabol. Dis. 2010; 9: 22.
• Ghazanfarpour E, Fatemi M, Ghandehari F. (2019). Protective effects of L. fermentum on lead induced hematological and body weight alterations in rats. Iranian. J. Toxicol. 13(3): 15-20
• International Agency for Research on Cancer (1993). Beryllium, cadmium, mercury and exposures in the glass manufacturing industry in 1993. 58. IARC, 119238, Lyon, France.
• Kısadere I, Aydin MF, Undag I. (2022). Partial protective effects of melatonin on cadmium-induced changes in hematological characteristics in rats. Biotech. Histochem. 2022; 97: 3.
• Kısadere I, Donmez N. (2019). The effects of quercetin on antioxidant system and some blood parameters in rats exposed to acute cadmium toxicity. Eurasian. J. Vet. Sci. 35 (2): 66-70.
• Kısadere I, Dönmez N, Dönmez HH. (2019). The effects of quercetin on antioxidant and cytokine levels in rat hippocampus exposed to acute cadmium toxicity. J. Cellular. Neurosci. Oxid. Stress. 11 (1): 10-10. https://doi.10.37212/jcnos.584684.
• Kısadere I, Karaman M, Aydın MF, Donmez N, Usta M. (2021). The protective effects of chitosan oligosaccharide (COS) on cadmium-induced neurotoxicity in Wistar rats. Arch. Environ. Occup. Health. 1-9. https://doi.org/10.1080/19338244.2021.2008852. Kısadere I. (2020). The effects of melatonin treatment on some serum immunoregulatory cytokine levels in rats exposed to chronic cadmium toxicity. Kocatepe. Vet. J.13 (3): 262-266.
• Kisadere I, Aydin MF, Dönmez HH. (2022). The influence of chitosan oligosaccharide on some hematological parameters in rats exposed to cadmium. Vet. Arhiv. 92 (1): 87-95, DOI: 10.24099/vet.arhiv.1319.
• Kisadere I, Kadyralieva N, Cihan H, Sur E, Dönmez N, Oruç E. (2017). Some physiological, hematological values and ANAE-positive lymphocyte rations of domestic donkeys (Equus asinus) in Kyrgyzstan. Kafkas. Univ. Vet. Fak. Derg. 23: 165-168. DOI: 10.9775/kvfd.2016.15784
• Liu Y, Li Y, Xia Y, Liu K, Ren L, Ji Y. (2020). The dysbiosis of gut microbiota caused by low-dose cadmium aggravate the injury of mice liver through increasing intestinal permeability. Microorganisms. 8: 211. https://doi.10.3390/microorganisms8020211.
• Ojekunle O, Banwo K, Sanni AI. (2017). In vitro and in vivo evaluation of Weissella cibaria and Lactobacillus plantarum for their protective effect against cadmium and lead toxicities. Letters. Applied. Microbiol. 64(5): 379-385.
• Ozaydın T, Çelik İ, Sur E, Öznurlu Y, Uluışık D. (2013). Cytochemistry of peripheral blood leukocytes in thorougbred foals. Biotec. Histochem. 88: 295-301. DOI: 10.3109/10520295.2013.782067
• Satarug S, Baker JR, Urbenjapol S, Haswell-Melkins M, Reilly PEB, Williams DJ, Moore MR. (2003). A global perspective on cadmium pollution and toxicity in non-occupationally exposed population. Toxicol. Letters. 137: 65-83. https://doi.org/10.1016/S0378-4274(02)00381-8.
• Satarug S, Garrett SH, Sens MA, Sens DA. (2010). Cadmium, environmental exposure, and health outcomes. Environ. Health. Perspect. 118 (2): 182-190. https://doi.org/10.1289/ehp.0901234. Sayed MGA, Farag EAH, Nasr HM. (2018). Effects of dietary probiotic (L. acidophilus) on hematology, biochemistry, and lipid profile of Carbon-Tetra chlorid induced toxicity in rats. Int. J. Sci Res. 9:1.
• Singh P, Pandey RK, Paswan VK, Yadav SP, Kumar BB, Shekhar SC. (2019). Effect of supplementation of L. plantarum and L. casei based probiotic milk powder on hematology, blood biochemistry and lipid profile of Charles Foster rats. Indian. J. Anim. Res. 53 (3):332-335.
• World Health Organization (2010). Exposure to cadmium: a major public health concern in 2010; p. 3-6.
• Zhai Q, Wang G, Zhao J, Liu X, Narbad A, Chen YQ, Zhang H, Tian F, Chen W. (2014). Protective effects of Lactobacillus plantarum CCFM8610 against chronic cadmium toxicity in mice: intestinal sequestration is not the only route of protection. Applied. Environ. Microbiol. 80: 4063-4071.