Effects of Donor Plant Age and Explant Types on Asparagus (Asparagus officinalis L.) Micropropagation

Yıl 2022, Cilt: 6 Sayı: 1, 12 - 17, 30.06.2022

Hatice Büşra Yavuz , Nuray Çömlekçioğlu

Öz

Asparagus (Asparagus officinalis L.) is a dioecious species in the Asparagaceae family. Male plants are more productive than female plants. More efficient plant propagation can be followed by focusing on the production of male plants with tissue culture techniques. The aim of this study is to determine the micro-propagation effects of shoot tip and lateral bud explants taken from donor plants of different ages (3 and 5 years). Jersey Knight F1 was used in the experiment. To obtain shoots from explants, 0.2 mg l-1 NAA (naphthen acetic acid), 0.2 mg l-1 BAP (benzyl amino purine), 0.2 mg l-1 kinetin, 30 g l-1 sucrose and 7 g l- 1 agar containing MS (Murashige and Skoog, 1962) medium was used. The shoots were transferred to proliferation MS medium containing 0.5 mg l-1 BAP and 0.2 mg l-1 IBA (indole butyric acid), and two subcultures were made. MS medium containing 0.5 mg l-1 IBA was used for rooting of the shoots. Shoot development was obtained from all cultured explants. In the first subculture, the average shoot numbers per explant obtained from 3 and 5 years old plants were determined as 7.0 and 7.18, respectively. While an average 9.43 shoots were obtained from shoot tip explant, 4.75 shoots from the lateral bud explant. In the second subculture, an average of 9.13 shoots from 3-year-old plants and 9.55 shoots from 5-year-old plants were obtained. While 10.53 shoots were obtained from the shoot tip, 8.15 shoots were detected from the lateral bud. The proliferation coefficient of the shoot tip explant in the first subculture differed significantly compared to the lateral bud explant. In contrast, the difference in proliferation coefficients decreased in the second subculture. 48.0% and 58.0% rooting rate were obtained from the explants of the 3 and 5 years old plants respectively. The average rooting were 65.0% in the shoot tip explant and 41.0% in the lateral bud explant.

Anahtar Kelimeler

Asparagus officinalis, micropropagation, donor age, shoot tip, axillary bud

Destekleyen Kurum

Scientific Research Projects Coordination Unit of Eskisehir Osmangazi University,

Proje Numarası

Project code: 202123A103

Kaynakça

• Açıkgöz, N., İlker, E., Gökçöl, A., 2004, Biyolojik Araştırmaların Bilgisayarda Değerlendirilmeleri, Ege Üniversitesi Tohum Teknolojisi Uyg. Ve Araş. Merkezi Yayınları No:2, Ege Üniversitesi Ziraat Fakültesi Ofset Atölyesi, Bornova-İzmir, 202s.
• Altunel, T. A. 2021, Morphological and Habitat Characteristics of Asparagus (Asparagus officinalis L.) and Socio-Economic Structure of Producers. Turkish Journal of Agriculture-Food Science and Technology, 9(6), 1092-1099.
• Anderson, T.W., 1984, An Introduction to Multivariate Statistical Analysis, Second Edition, John Wiley and Sons.
• Azad, M.A.K., Amin, M.N., 2017, Regeneration of Asparagus officinalis L. Through Embriyogenic Callus. Plant Tissue Culture and Biotech. 27(1): 21-31.
• Bojnauth, G., Puchooa, S., Bahorun, T., 2003, In vitro Regeneration of Asparagus officinalis: preliminary results. Food and Agriculture Research Council, Reduit, Mauritius, AMAS, pp. 7–15.
• Conner, A.J., Abernethy, D.J., Falloon, P.G., 1992, Importance of in vitro storage root development for the successful transfer of micropropagated asparagus plants to greenhouse conditions, New Zealand Journal of Crop and Horticultural Science, 20:4, 477-481.
• Culpupper, C.W., Moon, H.H., 1939, Changes in the composition and rate of growth along the developing stem of asparagus. Plant Physiology, 14:677-698.
• Desjardins, Y., 1992, Micropropagation of Asparagus (Asparagus officinalis L.). In: Bajaj Y.P.S. (eds) High-Tech and Micropropagation III. Biotechnology in Agriculture and Forestry, vol 19. Springer, Berlin, Heidelberg.
• Fortes, G., Muller, N., Augustin, E., Silva, J., Peters, J., Flores, R., 1997, The Influence of Spear Number on in vitro Rooting of Asparagus (Asparagus officinalis L.). HortScience. 32 (3), 471.
• Harada, T., Yakuwa, T., 1983, Studies on The Morphogenesis of Asparagus : Ⅵ. Effect of Sugar on Callus and Organ Formation in The in vitro Culture of Shoot Segments of The Seedlings. Journal of the Faculty of Agriculture, Hokkaido University, 61(3), 307-314.
• Inagaki, N., Harada, T., Yakuwa, T., 1981, Studies on The Anther Culture of Horticultural Crops: IV. Effect of Growth Regulators on Organ Formation From Anther Derived Callus of Asparagus officinalis L. J. Faculty Agric., Hokkaido University. 60 (3), 236–249.
• Khunachak, A., Chin, C., Le, T., Gianfagna, T., 1987, Promotion of asparagus shoot and root growth by growth retardants. Plant Cell, Tissue and Organ Culture. 11, 97–110.
• Kubota, S., Konno, I., Kanno, A., 2012, Molecular phylogeny of the genus Asparagus (Asparagaceae) explains interspecific crossability between the garden asparagus (A. officinalis) and other Asparagus species, Theor Appl Genet, 124, 345–354.
• Levi, A., Sink, K.C., 1991, Somatic Embryogenesis in Asparagus: The Role of Explants and Growth Regulators. Plant Cell Reports, 10:71-75.
• Maung, M., Myint, K.T., Thu, M.K., 2019, Effects of Different Explant Types, Plant Growth Regulators and Shoot Density on in vitro Regeneration of Asparagus (Asparagus officinalis L.). Journal of Agricultural Research, Vol. 6 (1), 109-115.
• Rasad, F. M., Hasbullah, N. A., Azis, N. A., Daud, N. F., Lassim, M. M., 2019, Micropropagation of Asparagus officinalis L. (Garden Asparagus) In Vitro.
• Paudel, N., Aryal, M.R., Puri, R.H., 2018, Effect of hormone for in vitro propagation of Asparagus racemosus Wild. Current Life Sciences, 4(4), 53-61.
• Pontaroli, A.C., Camadro, E.L., 2005, Plant Regeneration After Long Term Callus Culture in Clones of Asparagus officinalis L. Biocell. 29 (3), 313–317.
• Sallam, A.S., 2019, In vitro Propagation of F1 Male Hybrid Lines in Asparagus officinalis.Egyptian J. Desert Res., 69, No.1, 67-86.
• Sarabi, B., Almasi, K., 2010, Indirect organogenesis is useful for propagation of Iranian edible wild Asparagus (Asparagus officinalis L.). Asian Journal of Agricultural Sciences, 2(2), 47-50.
• Slabbert, M.M., Lindeque, J.M., Ferreira, D.I., 1990, Rapid in vitro multiplication of Asparagus. South African Journal of Botany, 56(3), 331-335.