CRONOBACTER SAKAZAKII: AN EMERGING PATHOGEN IN FOOD SAFETY

Yıl 2020, Cilt: 6 Sayı: 1, 54 - 63, 07.01.2020

Ülkü Demirci

Öz

Cronobacter sakazakii is a pathogen that causes fatal infections like necrotizing enterocolitis, and meningitis, especially in preterm and microsomic infants. Even though traces of the C. sakazakii has been detected in various food samples and other environmental sources, only contaminated powder infant formulas have been epidemiologically associated with infections caused by C. sakazakii. However, exposure to environmental and foodborne C. sakazakii species, pose a serious human health risk. Nevertheless, to be able to point out the importance of this issue and its widespread impact, there is a need for a significant number of studies about the detection of C. sakazakii species by rapid and sensitive molecular methods which are not available in our country for now.

The molecular examination of the virulence of each of the steps of food, such as production, harvesting, storage, processing, commercialization, and privatization, is important for the protection of consumer health and prevention of diseases caused by Cronobacteria. Molecular identification methods are one of the most important innovations for developing technology in the field of microbiology. The addition of new molecular identification techniques day by day is of great benefit to scientific studies carried out for different purposes.

Thus, this review was conducted to draw attention to public health risks that may be caused by the pathogenicity and virulence factors of the C. sakazakii.

Anahtar Kelimeler

Cronobacter sakazakii, Molecular Identification, Food Safety

Kaynakça

• Almajed, F. S., & Forsythe, S. J. (2016). Cronobacter sakazakii clinical isolates overcome host barriers and evade the immune response. Microbial Pathogenesis, 90, 55–63. doi:10.1016/j.micpath.2015.11.014. Cava Gümüş, P., Tekiner, İ. H., Çakmak, B., Tacer Caba, Z., & Özpınar, H. (2017). Investigation of extended spectrum Β-lactamases (ESBL)-producing Enterobacteriaceae and Cronobacter Spp in infant formulas and cereal-based foods for children. İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi, 19-32. Demirci, U., Tekiner, İ. H., Çakmak, B., Özpınar H. (2018). Occurrence and molecular characterization of different virulence-associated genes of Cronobacter sakazakii isolates from some foods and dust samples. Cienc. Rural vol.48 no.8. doi: 10.1590/0103-8478cr20180127 Du, X., Han, R., Li, P., & Wang, S. (2015). Comparative proteomic analysis of Cronobacter sakazakii isolates with different virulences. Journal of Proteomics, 128, 344–351. doi:10.1016/j.jprot.2015.08.013. El-Sharoud, W. M., O’Brien, S., Negredo, C., Iversen, C., Fanning, S., & Healy, B. (2009). Characterization of Cronobacter recovered from dried milk and related products. BMC Microbiology, 9(1), 24. doi:10.1186/1471-2180-9-24. Erkekoglu, P., Sipahi, H., Şahin, G., & Baydar, T. A. (2009). Hidden danger in infant formulas and baby foods: Enterobacter sakazakii contamination. FABAD J Pharm Sci, 34, 153-65.
• Eshwar, A. K., Tall, B. D., Gangiredla, J., Gopinath, G. R., Patel, I. R., Neuhauss, S. C. F., Lehner, A. (2016). Linking Genomo- and Pathotype: Exploiting The Zebrafish Embryo Model To Investigate The Divergent Virulence Potential Among Cronobacter Spp. Plos One, 11(6), E0158428. Doi:10.1371/Journal.Pone.0158428. FAO/WHO, “Enterobacter sakazakii (Cronobacter spp.) in powdered follow-up formulae”, Microbiological Risk Assessment Series No. 15. Rome. 90pp. Retrieved from http://www.who.int/foodsafety (05/2019) Fei, P., Man, C., Lou, B., Forsythe, S. J., Chai, Y., Li, R., Jiang, Y. (2015). Genotyping and Source Tracking of Cronobacter sakazakii and C. malonaticus Isolates from Powdered Infant Formula (PIF) and an Infant Formula Production Factory in China. Applied and Environmental Microbiology, 81(16), 5430–5439. doi:10.1128/aem.01390-15. Heperkan, D., Dalkilic-Kaya, G., & Juneja, V. K. (2017). Cronobacter sakazakii in baby foods and baby food ingredients of dairy origin and microbiological profile of positive samples. LWT, 75, 402–407. doi:10.1016/j.lwt.2016.09.013. Hunter, C. J., & Bean, J. F. (2013). Cronobacter: an emerging opportunistic pathogen associated with neonatal meningitis, sepsis and necrotizing enterocolitis. Journal of Perinatology, 33(8), 581–585. doi:10.1038/jp.2013.26. Ivy, R. A., Farber, J. M., Pagotto, F., & Wiedmann, M. (2013). International Life Science Institute North America Cronobacter (Formerly Enterobacter sakazakii) Isolate Set. Journal of Food Protection, 76(1), 40–51. doi:10.4315/0362-028x.jfp-11-546. Jaradat, Z. W., Ababneh, Q. O., Saadoun, I. M., Samara, N. A., & Rashdan, A. M. (2009). Isolation of Cronobacter spp. (formerly Enterobacter sakazakii) from infant food, herbs and environmental samples and the subsequent identification and confirmation of the isolates using biochemical, chromogenic assays, PCR and 16S rRNA sequencing. BMC Microbiology, 9(1), 225. doi:10.1186/1471-2180-9-225. Kim, K., Kim, K. P., Choi, J., Lim, J. A., Lee, J., Hwang, S., & Ryu, S. (2010). Outer membrane proteins A (OmpA) and X (OmpX) are essential for basolateral invasion of Cronobacter sakazakii. Appl. Environ. Microbiol., 76(15), 5188-5198. Miranda, N., Banerjee, P., Simpson, S., Kerdahi, K., & Sulaiman, I. (2017). Molecular Surveillance of Cronobacter spp. Isolated from a Wide Variety of Foods from 44 Different Countries by Sequence Typing of 16S rRNA, rpoB and O-Antigen Genes. Foods, 6(5), 36. doi:10.3390/foods6050036. Müller, A., Stephan, R., Fricker-Feer, C., & Lehner, A. (2013). Genetic Diversity of Cronobacter sakazakii Isolates Collected from a Swiss Infant Formula Production Facility. Journal of Food Protection, 76(5), 883–887. doi:10.4315/0362-028x.jfp-12-521. Ogihara, H., Kiribe, N., Fukuda, N., Furukawa, S., Morinaga, Y., & Igimi, S. (2014). Cronobacter spp. in Commercially Available Dried Food in Japan. Biocontrol Science, 19(4), 209–213. doi:10.4265/bio.19.209. Parra-Flores, J., Rodriguez, A., Riffo, F., Arvizu-Medrano, S. M., Arias-Rios, E. V., & Aguirre, J. (2015). Investigation on the Factors Affecting Cronobacter sakazakii Contamination Levels in Reconstituted Powdered Infant Formula. Frontiers in Pediatrics, 3. doi:10.3389/fped.2015.00072. Shaker, R., Osaili, T., Al-Omary, W., Jaradat, Z., & Al-Zuby, M. (2007). Isolation of Enterobacter sakazakii and other Enterobacter sp. from food and food production environments. Food Control, 18(10), 1241–1245. doi:10.1016/j.foodcont.2006.07.020. Ye, Y., Li, H., Ling, N., Han, Y., Wu, Q., Xu, X., Gao, J. (2016). Identification of potential virulence factors of Cronobacter sakazakii isolates by comparative proteomic analysis. International Journal of Food Microbiology, 217, 182–188. doi:10.1016/j.ijfoodmicro.2015.08.025. Zimmermann, J., Schmidt, H., Loessner, M. J., & Weiss, A. (2014). Development of a rapid detection system for opportunistic pathogenic Cronobacter spp. in powdered milk products. Food Microbiology, 42, 19–25. doi:10.1016/j.fm.2014.02.010.