Production Of Tetracycline Hydrochloride-Collagen-Doped Chitosan Nanofiber Scaffolds And Investigation Of Their Antibacterial Properties

Öz

Çalışmanın amacı kitosan doku iskelesine tetrasiklin hidroklorür ve kollajen hemostatik ajan yükleyerek enfeksiyon riski olmadan iyileşme sürecini hızlandırabilen asil bir biyomateryal geliştirmektir. Denemelerden sonra, elektro eğirme ile kitosan ve PVA'dan iyi bir doku iskelesi elde edildi. Bu biyomateryalin hemostatik özelliklerini arttırmak için, PVA kitosan karışımına %10 (ağırlıkça) kollajen hemostatik ajan eklendi. Kitosan doku iskelesindeki kollajen hemostatik ajan miktarı ayarlandıktan sonra çeşitli miktarlarda tetrasiklin eklenmiş ve antibakteriyel ve yara iyileştirici özelliklerini artırmak için 5 farklı biyomateryal geliştirilmiştir. Biyomateryaldeki antibiyotik konsantrasyonu birinci örnekte IV %10, ikinci örnekte %15, üçüncü örnekte %20, dördüncü örnekte %25 ve beşinci örnekte %30 idi. Son olarak, elde edilen biyomalzemelerin hastane bakterileri üzerindeki etkileri (gram pozitif: Staphylococcus Aureus, gram negatif: Pseudomonas Aeruginosa) Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Mikrobiyoloji Anabilim Dalı laboratuvarlarında in vitro testler ile analiz edilmiştir. İnceleme sonucunda birinci, üçüncü ve beşinci günlerde biyomalzemenin ne kadar olması gerektiği ve bakteri üremesine karşı ne kadar etkili olduğu incelenmiştir. Biyomateryalin acil durumlarda ve cerrahi işlemlerde çok etkili olacağı düşünülmektedir.

Anahtar Kelimeler

• Chitosan
• Collagen
• Hemostatic agent
• Biomaterials
• Scaffold

Production Of Tetracycline Hydrochloride-Collagen-Doped Chitosan Nanofiber Scaffolds And Investigation Of Their Antibacterial Properties

Öz

The aim of the study is to develop a noble biomaterial that can accelerate the healing process without the risk of infection by loading tetracycline hydrochloride and collagen hemostatic agent into the chitosan tissue scaffold. After the trials, a good tissue scaffold was obtained from chitosan and PVA by electro spinning. To increase the hemostatic features of this biomaterial, 10% (by weight) collagen hemostatic agent was added to the PVA chitosan blend. After the amount of collagen hemostatic agent in the chitosan tissue scaffold was set, various amounts of tetracycline were added and 5 different biomaterials were developed to augment the antibacterial and wound healing properties. Antibiotic concentration in the biomaterial was IV 10% in the first, 15% in the second, 20% in the third, 25% in the fourth and 30% in the fifth sample. Finally, the effects of the obtained biomaterials on the nosocomial bacteria (gram positive: Staphylococcus Aureus, gram negative: Pseudomonas Aeruginosa) were analyzed with in-vitro tests at Kahramanmaras Sutcu Imam University, School of Medicine Department of Microbiology laboratories. As a result of the examination, it was examined how much the biomaterial should be and how effective it was against bacterial growth on the first, third and fifth days. It is thought that biomaterial will be very effective in emergencies and surgical procedures.

Anahtar Kelimeler

• Chitosan
• Collagen
• Hemostatic agent
• Biomaterials
• Scaffold

Kaynakça

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