Development of a UV-Vis Spectroscopic Method for Etodolac Determination and Evaluation of Pharmaceutical Additive Effects

Öz

Etodolac is an active substance from the nonsteroidal anti-inflammatory drug (NSAID) class and is widely used in the treatment of pain and inflammation. In this study, a simple, rapid, and sensitive UV-Vis spectrophotometric method was developed and validated for the quantitative determination of etodolac from commercial tablet formulations. Etodolac showed maximum absorbance at 278 nm. Methanol was optimized as the most suitable solvent in the study, and the method was validated according to these conditions. The method demonstrated a linear working range of 0.88- 40 µg mL-1. The limit of detection (LOD) was found to be 0.29 µg mL-1. Additionally, the effects of some pharmaceutical excipients on the UV absorbance of etodolac were investigated. Pharmaceutical excipients can cause changes in the UV absorbance of the active ingredient, which can lead to incorrect dosage determinations and analytical errors. In this context, the effect of mannose, lactose monohydrate, and potassium sorbate on the maximum absorbance of etodolac at 278 nm was evaluated, and possible interferences were examined. In addition, stability studies were carried out for four weeks to test the long-term reliability of the analysis method. Moreover, the recovery of etodolac from the Etotio tablet formulation was measured, and the method's accuracy was evaluated. The data showed that the applied analysis method offers high precision, reproducibility, and reliability. This study presents an optimized, sensitive, and selective method for accurately quantifying etodolac in pharmaceutical preparations.

Anahtar Kelimeler

• Etodolac
• Analytical method
• UV-Vis spectroscopy
• Pharmaceutical analysis
• Drug additives

Etodolak Tayini ve Farmasötik Katkı Maddesi Etkilerinin Değerlendirilmesi için UV-Vis Spektroskopik Yöntem Geliştirilmesi

Öz

Etodolak, nonsteroidal antiinflamatuar ilaç (NSAID) sınıfından bir aktif maddedir ve ağrı ve inflamasyon tedavisinde yaygın olarak kullanılır. Bu çalışmada, ticari tablet formülasyonlarından etodolakın kantitatif tayini için basit, hızlı ve hassas bir UV-Vis spektrofotometrik yöntem geliştirildi ve doğrulandı. Etodolak 278 nm'de maksimum absorbans gösterdi. Metanol, çalışmada en uygun çözücü olarak optimize edildi ve yöntem bu koşullara göre doğrulandı. Yöntem, 0,88-40 µg mL-1'lik doğrusal bir çalışma aralığı gösterdi. Tespit sınırı (LOD) 0,29 µg mL-1 olarak bulundu. Ayrıca, bazı farmasötik yardımcı maddelerin etodolak'ın UV absorbansı üzerindeki etkileri araştırıldı. Farmasötik yardımcı maddeler, aktif maddenin UV absorbansında değişikliklere neden olabilir, bu da yanlış dozaj belirlemelerine ve analitik hatalara yol açabilir. Bu bağlamda, mannoz, laktoz monohidrat ve potasyum sorbatın etodolakın 278 nm'deki maksimum absorbansı üzerindeki etkisi değerlendirildi ve olası girişimler incelendi. Ayrıca, analiz yönteminin uzun vadeli güvenilirliğini test etmek için dört hafta boyunca stabilite çalışmaları yürütüldü. Dahası, Etotio tablet formülasyonundan etodolak geri kazanımı ölçüldü ve yöntemin doğruluğu değerlendirildi. Veriler, uygulanan analiz yönteminin yüksek hassasiyet, tekrarlanabilirlik ve güvenilirlik sunduğunu gösterdi. Bu çalışma, farmasötik preparatlarda etodolakın doğru bir şekilde ölçülmesi için optimize edilmiş, hassas ve seçici bir yöntem sunmaktadır

Anahtar Kelimeler

• Etodolak
• Analitik yöntem
• UV-Vis spektroskopisi
• İlaç analizi
• İlaç katkı maddeleri

Kaynakça

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