Ayçiçeğinde (Helianthus annuus L.) GPAT Gen Ailesinin Genom Çapında Tanımlanması, Evrimsel Analizi ve Kapsamlı Bilgisayar Destekli Karakterizasyonu

Öz

Gliserol-3-fosfat açiltransferaz (GPAT), gliserolipit biyosentezinde rol alan ve bitki büyümesi, gelişimi ve abiyotik stres tepkilerinde önemli bir rol oynayan enzimlerdir. Bununla birlikte, ayçiçeğinde (Helianthus annuus L.) GPAT gen ailesinin kapsamlı bir genom çapında ve in silico analizi bugüne kadar yapılmamıştır. Bu çalışmada, ayçiçeği genomundaki GPAT gen ailesi biyoinformatik yaklaşımlar kullanılarak karakterize edilmiştir. Toplam 23 HaGPAT geni tanımlanmıştır; bunların kromozomal dağılımları, filogenetik ilişkileri, gen yapıları, korunmuş motifleri, protein özellikleri, hücre altı lokalizasyonları, cis-düzenleyici elementleri, miRNA hedefleri, protein-protein etkileşim ağları, üç boyutlu protein yapıları, senteni ilişkileri, duplikasyon olayları, Ka/Ks oranları ve RNA-seq verilerine dayalı ekspresyon profilleri analiz edilmiştir. RNA-seq verilerine dayalı ekspresyon analizleri, HaGPAT genlerinin farklı dokularda değişken ekspresyon kalıpları sergilediğini göstermiştir. Bu bulgular, HaGPAT gen ailesinin yapısal özelliklerinin, evrimsel ilişkilerinin ve ekspresyon profillerinin daha iyi anlaşılmasına katkıda bulunmakta ve gelecekteki fonksiyonel çalışmalar için değerli bir genomik kaynak oluşturmaktadır.

Anahtar Kelimeler

• Helianthus annuus
• GPAT
• Genom çapında analiz
• RNA-sekans analizi

Etik Beyan

Çalışma etik standartlara uygundur.

Teşekkür

Yazar, Türkiye Bilim ve Teknolojik Araştırma Kurumu'na (TÜBİTAK) 2211-A burs programı aracılığıyla sağladığı destek için teşekkür eder.

Genome-Wide Identification, Evolutionary Analysis and Comprehensive In Silico Characterization of the GPAT Gene Family in Sunflower (Helianthus annuus L.)

Öz

Glycerol-3-phosphate acyltransferase (GPAT) are enzymes involved in glycerolipid biosynthesis and play a key role in plant growth, development, and abiotic stress responses. However, a comprehensive genome-wide and in silico analysis of the GPAT gene family in sunflower (Helianthus annuus L.) has not been performed to date. In this study, the GPAT gene family in the sunflower genome was characterized using bioinformatics approaches. A total of 23 HaGPAT genes were identified; their chromosomal distributions, phylogenetic relationships, gene structures, conserved motifs, protein properties, subcellular localizations, cis-regulatory elements, miRNA targets, protein-protein interaction networks, three-dimensional protein structures, synteny relationships, duplication events, Ka/Ks ratios, and expression profiles based on RNA-seq data were analyzed. Expression analyses based on RNA-seq data showed that HaGPAT genes exhibited variable expression profiles under different tissues. These findings contribute to a better understanding of the structural features, evolutionary relationships, and expression profiles of the HaGPAT gene family, and constitute a valuable genomic resource for future functional studies.

Anahtar Kelimeler

• Helianthus annuus
• GPAT
• genom-wide analysis
• RNA-seq

Etik Beyan

The study is proper with ethical standards.

Teşekkür

Author would like to thank the Scientific and Technological Research Council of Turkey (TÜBİTAK) for support through the 2211-A scholarship program.

Kaynakça

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