Araştırma Makalesi

Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia

Cilt: 3 Sayı: 3 15 Aralık 2020
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Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia

Öz

Antibiotic resistant bacteria have been a major concern due to the problems that they may pose in terms of public and environmental health. This present study aimed to investigate the presence of antibiotic resistance among bacterial strains isolated from rivers and to determine the impact of various human activities on the distribution of antibiotic resistant bacteria. Bacteria were isolated from river water samples of Lebir River (Point 1 and Point 2) and Kelantan River (Point 3 and Point 4). Antibiotic susceptibility test was conducted on the bacterial isolates against six types of antibiotics (ampicillin, gentamycin, tetracycline, chloramphenicol, rifampicin and ciprofloxacin) was performed, followed by characterization and identification of the isolates using a series of biochemical tests. The findings show the presence of antibiotic resistant bacteria in all four samples, indicating the relationship between the resistance of isolates and the human activities within the vicinity of the area. A total of 27 bacterial isolates were successfully isolated from the rivers and predicted as Actinomyces sp., Arachnia sp., Streptomyces sp., Streptococcus sp., Enterococcus sp., Bacillus sp., Clostridium sp., Neisseria sp., Veillonella sp., Bacteroides sp., Salmonella sp., Shigella sp., Proteus sp., Enterobacter sp., Klebsiella sp., Escherichia sp, Pseudomonas sp., Aeromonas sp., Vibrio sp. Staphylococcus sp., Micrococcus sp., Actinobacillus sp., and Flavobacterium sp. However, further analysis of the antibiotic resistant bacteria is recommended to further identify and understand them due to the potential risk that they may pose to human and environment.

Anahtar Kelimeler

Destekleyen Kurum

Universiti Teknologi Malaysia

Proje Numarası

17H74 and 04G97

Teşekkür

We sincerely thank the staff and administration of the Universiti Teknologi Malaysia for their assistance and support in this study.

Kaynakça

  1. 1. Jiang, L., et al., Prevalence of antibiotic resistance genes and their relationship with antibiotics in the Huangpu River and the drinking water sources, Shanghai, China. Science of the Total Environment. 2013. 458: p. 267–272.
  2. 2. Martinez, J.L., Environmental pollution by antibiotics and by antibiotic resistance determinants. Environmental Pollution, 2009. 157: p. 2893–2902.
  3. 3. Al-Badaii, F., and M.S. Othman, Water Pollution and its Impact on the Prevalence of Antibiotic-Resistant E. coli and Total Coliform Bacteria: A Study of the Semenyih River, Peninsular Malaysia. Water Qual Expo Health, 2015. 7: p. 319–330.
  4. 4. Barile, M.F., Gram staining technique. Methods in Mycoplasmology V1: Mycoplasma Characterization, 2012. 1: p. 39.
  5. 5. Hudzicki, J., Kirby-Bauer disk diffusion susceptibility test protocol. American Society for Microbiology, 2009. 2016: p. 1-23.
  6. 6. Clinical and Laboratory Standards Institute, CLSI, M100-S25. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. Wayne, PA: Clinical and Laboratory Standards Institute, 2015.
  7. 7. Bhuvaneshwari, G., Multiple Antibiotic Resistance Indexing of Non-Fermenting Gram-Negative Bacilli. Asian Journal of Pharmaceutical and Clinical Research, 2017. 10 (6): p. 78-80.
  8. 8. Holt, J. G., S. T. Williams, and Holt, Bergey's Manual of Systematic Bacteriology, Vol. 4: Lippincott Williams & Wilkins, 1989.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Mikrobiyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Nurul Liyana Hassan Bu kişi benim
Malaysia

Kam Kar Yern Bu kişi benim
Malaysia

Yayımlanma Tarihi

15 Aralık 2020

Gönderilme Tarihi

1 Nisan 2020

Kabul Tarihi

8 Ağustos 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 3 Sayı: 3

Kaynak Göster

APA
Hassan, N. L., Kar Yern, K., & Mohd Zain, N. A. (2020). Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia. International Journal of Life Sciences and Biotechnology, 3(3), 291-307. https://doi.org/10.38001/ijlsb.712542
AMA
1.Hassan NL, Kar Yern K, Mohd Zain NA. Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia. Int J. Life Sci. Biotechnol. 2020;3(3):291-307. doi:10.38001/ijlsb.712542
Chicago
Hassan, Nurul Liyana, Kam Kar Yern, ve Nor Azimah Mohd Zain. 2020. “Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia”. International Journal of Life Sciences and Biotechnology 3 (3): 291-307. https://doi.org/10.38001/ijlsb.712542.
EndNote
Hassan NL, Kar Yern K, Mohd Zain NA (01 Aralık 2020) Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia. International Journal of Life Sciences and Biotechnology 3 3 291–307.
IEEE
[1]N. L. Hassan, K. Kar Yern, ve N. A. Mohd Zain, “Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia”, Int J. Life Sci. Biotechnol., c. 3, sy 3, ss. 291–307, Ara. 2020, doi: 10.38001/ijlsb.712542.
ISNAD
Hassan, Nurul Liyana - Kar Yern, Kam - Mohd Zain, Nor Azimah. “Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia”. International Journal of Life Sciences and Biotechnology 3/3 (01 Aralık 2020): 291-307. https://doi.org/10.38001/ijlsb.712542.
JAMA
1.Hassan NL, Kar Yern K, Mohd Zain NA. Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia. Int J. Life Sci. Biotechnol. 2020;3:291–307.
MLA
Hassan, Nurul Liyana, vd. “Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia”. International Journal of Life Sciences and Biotechnology, c. 3, sy 3, Aralık 2020, ss. 291-07, doi:10.38001/ijlsb.712542.
Vancouver
1.Nurul Liyana Hassan, Kam Kar Yern, Nor Azimah Mohd Zain. Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia. Int J. Life Sci. Biotechnol. 01 Aralık 2020;3(3):291-307. doi:10.38001/ijlsb.712542

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