Antimicrobial Activity of Musa acuminata Peel Extract against Gram-Positive Bacteria

Öz

Particular communities are discarding banana (Musa acuminata) peels as household and industrial food waste. It is high in nutritive value and a healthy source of food to consume. This study aims to evaluate the antimicrobial activity of M. acuminata peels against four types of Gram-positive bacteria, namely, Bacillus cereus, Staphylococcus aureus, Streptococcus pneumoniae and Streptococcus pyogenes. The extract of M. acuminate peel was prepared using a soxhlet apparatus for ethanolic extract and rotary evaporator to concentrate the ethanolic extract. Antimicrobial activity was carried out on the extract using agar disc diffusion technique; the inhibitory zones were recorded in millimetres. Results showed that the disc diffusion method of ethanolic extract of M. acuminata peel was not effective against all the Gram-positive bacteria strains. This result indicated that M. acuminata peel did not pose any potential antimicrobial effect against Gram-positive bacteria. However, further study should be carried out using different extraction method and concentration in order to evaluate antimicrobial activity.

Anahtar Kelimeler

• Antimicrobial activity
• Gram-positive bacteria
• Musa acuminata peel

Kaynakça

1. Abdel Rahman, S., Abd-Ellatif, S., Deraz, S. & Khalil, A. (2011). Antibacterial activity of some wild medicinal plants collected from western Mediterranean coast, Egypt: Natural alternatives for infectious disease treatment. Biotechnology, 10(52), 10733-10743
2. Ahmed, S., Ahmad, M., Swami, B. L. & Ikram, S. (2016). A review on plants extract mediated synthesis of siver nanoparticles for antimicrobial applications: A green expertise. Advanced Research, 7(1), 17-28
3. Andrade, R. J. & Tulkens, P. M. (2011). Hepatic safety of antibiotics used in primary care. Antimicrobial Chemotheraphy, 66(7), 1431-1446
4. Anem, M. (2012, October 8). Anim agriculture technology. Retrieved from www.animagrow.blogspot.my/2012/10/growing-banana-in-malaysia.html?m=1
5. Atzingen, D. A., Gragnani, A., Veiga, D. F., Abla, L. E., Mendonca, A. R., Paula, C. A., Juliano, Y. Correa, J. C., Faria, M. R. & Ferreira L. M. (2011). Gel from unripe Musa sapientum peel to repair surgical wounds in rats, Acta Cirurgica Brasileira, 26, 379-382
6. Baskar, R., Shrisakthi, S., Sathyapriya, B., Shyampriya, R., Nithya, R. and Poongodi, P. (2011). Antioxidant potential of peel extracts of banana varieties (Musa sapientum). Food and Nutrition Sciences, 02(10), 1128-1133.
7. Brown, L., Wolf, J. M., Prados-Rosales, R. & Casadevall, A. (2015, September 1). Through the wall: extracellular vesicles in Gram0positive bacteria, mycobacteria and fungi. Nature Review Microbiology, doi: 10.1038/nrmicri3480
8. Budin, G., Chung, H. J., L, H & Weissleder, R. (2012). A magnetic gram stain for bacterial detection. Angewandte Chemie International Edition in English, 51(31), 7752-7755

9. Chan, A., Sherman, P. & Bourke, B. (2006). Clinical infectious diseases. Oxford Academics, 43(9), 1230-1231. doi: 10.1086/508893
10. Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbilogy Reviews, 12(4), 564-582
11. Daniel, M. (2007). Medicinal plants: chemistry and properties. Enfield (New Hampshire): Science Publishers, 82(2), 1, Retrieved from www.scribd.com/doc/1444207357/Medicinal-Plants-Chemistry-and-Properties-M-Daniel
12. Edenta, C., James, D. B., Owolabi, O. A. & Okoduwa, S. I. R. (2014). Hypolipidemic effects of aqueous extract of three cultivars of Musa sapientum fruit peel on polarxamer-407 induced hyperlipidemic wistar rats. Pharmacology and Science Research, 5, 1046-1051
13. Edenta, C., Okoduwa, S. and Okpe, O. (2017). Effects of aqueous extract of three cultivars of banana (Musa acuminata) fruit peel on kidney and liver function indices in wistar rats. Medicines, 4(4), 1-7.
14. Ehiowemwenguan, G., Emoghene, A. O. & Inetianbor, J. E. (2013). Antibacterial and phytochemical analysis of banana fruit peel. Pharmacy, 4(8), 18-25
15. Emaga, T. H., Ronkart, S. N., Wathelet, B. & Paquot, M. (2008). Characterisation of pectins extracted from banana peels (Musa AAA) under different conditions using an experimental design. Food Chemistry, 108, 463-471
16. Fatemah, S. R., Saifullah, R., Abbas, F. M. A. & Azhar, M. E., (2012). Total phenolic and antioxidant activity of banana pulp and peel flours: Influence of variety and stage of ripeness. International Food Research, 19(3), 1041-1046.
17. Fukuyama, H., Yamashiro, S., Kinjo, K., Tamaki, H. & Kishaba, T. (2014). Validation of sputum Gram stain for treatment of community-acquired pneumonia and healthcare-associated pneumonia: A propective observational study. Infectious Diseases, 14(534), 1-8
18. Grare, M., Fontanay, S., Cornil, C., Finance, C. & Duval, R. E. (2008). Tetrazolium salts for MIC determination in microplates: Why? Which salt to select? How?. Microbiological Methods 75(1), 156-159
19. Griffth, F. (1934). The serological classification of Streptococcus pyogenes. Hygiene, 34(4), 542-584
20. Hoffmar, a., Hill, K., Gee, J., Marstone, C., De., B., Popovic, T., Sue, D., Wilkins, P., Avashia, S., Drumgoole, R., Helma, C., Ticknor, L., Okinaka, R. & Jackson, J. (2006). Characterization of Bacillus cereus isolates associated with fatal pneumonias: Strains are closely related to Bacillus anthracis and harbor B. anthracis virulence. Clinical Microbiology (2006). 44(9), 3352-3360
21. Holmes, A. H., Moore, L. S. P., Sundsfiord, A., Steinbakk, M., Regmi, S. Karkey, A., Guerin, P. J. & Piddock, L. J. V. (2015). Antimicrobials: access and sustainable effectiveness 2: Understanding the mechanisms and drivers of antimicrobial resistance. Series, 1-12
22. Huttner, B., Samore, M. (2011). Outpatient antibiotic use in the United States: time to “get smarter”. Clinical Infectious Diseases, 53(7), 540-543
23. Khan, Z. Z. & Salvaggio, M. R. (2017, August 8). Group A Streptococcal (GAS) infections. Retrieved from www.emedicine.medscape.com/article/228936-overview
24. Kumar, K. P. S., Bhowmik, D., Duraivel, S. & Umadevi, M. (2012). Traditional and Medicinal Uses of Banana. Pharmacology and phytochemical, 1, 51-63 L al, N., Sahu, N., Shiurkar, G., Kumar, J. D., & Chack, S. (2017). Banana: awesome fruit crop for society. The Pharmacology Innovation, 6(7), 223-228.
25. Lanie, J. Ng. W., Kazmierczak, K., Andrzejewski, T., Davidsen, T., Wayne, K., Tettelin, H., Glass, J., Winkler, M. Genome sequence of Avery’s virulent serotype 2 strain D39 of Streptococcus pneumonia and comparison with that of unencapsulated laboratory strain R6. Bacteriology, 189, 38-51
26. Leekha, S., Terrell, C. L. & Edson, R. S. (2011). General principle of Antimicrobial Therapy. Mayo Clinic Proceedings, 86(2), 156-167
27. Levy, S. B. & Marshall, B. (2004). Antibacterial resistance worldwide: causes, challenges and responses. National Medicine, 10(12), 122-129
28. Lowy, F. D. (2003). Antimicrobial resistance: the example of Staphylococcus aureus. Clinical Investigation, 111(9), 1265-1273
29. Mohapatra, D., Mishra, S., & Sutar, N. (2010). Banana and its by-product utilization: an overview. Scientific & Industrial Research, 69, 323-329.
30. Mosa, Z. M. & Khalil, A. F. (2015). The effects of banana peels supplements diet on acute liver failure rats. Agriculture sciences, 60(2), 373-379
31. Mostafa, A. A., Al-Askar, A. A., Almaary, K. S., Dawoud, T. M., Sholkamy, E. N. & Bakri, M. M. (2018). Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Biological Sciences, 25(1), 361-366
32. Navghare, V. and Dhawale, S. (2017). In vitro antioxidant, hypoglycemic and oral glucose tolerance test of banana peels. Medicine, 53(3), 237-243.
33. Patel, J. B., Weinstein, M. P., Elipoulos, G. M., Jenkins, S. G., Lewis, J. S., Limbago, B., Mathers, A. J., Mazzulli, T., Patel, R., Richter, S. S., Satlin, M., Swenson, J. M., Traczewski, M.
34. M., Turnidge, J., D. & Zimmer, B., L. (2017). Performance standards for antimicrobial susceptibility testing. Clinical Laboratory Standards Institute, 37(27), 1-249
35. Pereira, A. and Maraschin, M. (2015). Banana (Musa spp) from peel to pulp: Ethnopharmacology, source of bioactive compounds and its relevance for human health. Ethnopharmacology, 160, 149-163
36. Pereira, M. G., Maciel, G. M., Haminiuk, C. W. I., Bach, F., Hamerski, F., Scheer, A. P. & Corazza, M. L. (2018). Effect of extraction process on composition, antioxidant and antibacterial activity of oil from yellow passion fruit (Passiflora edulis Var. Flavicarpa) seeds. Waste and Biomass Valorization, 1-15
37. Prakash, B., Sumangala, C. H., Melappa, G. & Gavimath, C. (2016). Evaluation of antifungal activity of banana peel against scalp fungi. Materials Today: Proceedings, 4(2017), 11977-11983
38. Priyanga, S., Hemmalakshmi, S., Vidya, B., Chella Perumal, P., Gopalakrishnan, V. K., & Devaki, K. (2017). Acute toxicity assessment of ethanolic leaf extract of Macrotyloma uniflorum L. on wistar albino rats. Toxicology International Formerly Indian, 24(1), 22. doi:10.22506/ti/2017/v24/i1/149029
39. Rajakannu, S., Shankar, S., Perumal, S., Subramanian, S. & Dhakshinamoorthy, G. P. (2015). Biosynthesis of silver nanoparticles using Garcinia mangostana fruit extract and their antibacterial, antioxidant activity. Current Microbiology and Applied Science, 4(1), 944-952
40. Rattanavichi, W. & Cheng, Winton. (2014). Effects of hot-water extract of banana (Musa acuminata)fruit’s peel on the antibacterial activity, and anti-hypothermal stress, immune responses and disease resistance of the giant freshwater prawn, Macrobrachium resenbegii. Fish & Shellfish Immunology, 39(1), 326-335
41. Reddy, G.V., Babu, P. R., Komaraiah, P., Roy, K. R. R. M. & Kothari, I. L. (2003). Utilization of banana waste for theproduction of lignolytic and cellulolytic enzymes by solid substrate fermentation using two Pleurotus species (P. ostreatus and P. sajor-caju). Process Biochemistry, 38(10), 1457-1462
42. Srinivasan, D., Nathan, S. Suresh, T. & Perumalsamy, P. L. (2001). Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. Ethnopharmacology, 74(2001), 217-220.
43. Singhal, M. & Ratra, Purnima. (2013). Antioxidant activity, total flavonoid and total phenolic content of Musa acuminata peel extracts. Pharmacology, 7(2), pp118-122.
44. Sirajudin, Z. N. M., Ahmed, Q. U., Chowdhury, A. J. K., Kamarudin, E. Z., Khan, A. V., Uddin, A. H. & Musa, N. (2014). Antimicrobial activity of Banana (Musa paradisiaca L.) peels against food borne pathogenic microbes. Pure and Applied Microbiology, 8(5), 3627-3639
45. Shankar, G., Jeevitha, P. & Shadeesh, L. (2017). Nutritional analysis of Musa acuminata. Food and DairyTechnology, 5(4), pp27-29.
46. Someya, S., Yoshiki, Y., & Okubo, K. (2002). Antioxidant compounds from bananas (Musa Cavendish). Food Chemistry, 79(3), 351-354. doi: 10.1016/s0308-8146(02)00186-3
47. Stoppler, M. C. (2017). Staph infection (Staphylococcus aureus). Medicine Net. Retrieved from www.medicinenet.com/staph_infection/article.html#staph_infection_facts
48. Sumathy, V., Lachumy, S. J., Zakaria, Z. & Sasidharan, S. (2011). In vitro bioactivity and phytochemical screening of Musa acuminata flower. Pharmacologyonline, 2, 118-127
49. Tingpej, B. (2014). Bacillus. National Library of Medicine. MeSH Scope Note and Classification. Retrieved from www.idimages.org/m/atlas/organism/?atlasentryID=93&organism=Bacillus
50. Todar, K. (2003). Streptococcus pneumonia. Textbook of Bacteriology, Retrieved from www.textbookofbacteriology.net/S.pneumoniae
51. Vilain, S., Luo, Y., Hildreth, M. & Brozel, V. 2006). Analysis of the life cycle of the soil saprophyte bacillus cereus in liguid soil ectract and in soil. Applied Environmental Microbiology, 72(7), 4970-4977
52. Wijnands, L., Dufrenne, J., Zwietering, M. H., and Leusden, F. (2006). Spores from mesophilic Bacillus cereus strains germinate better and grow faster in simulated gastro-intestinal conditions than spores from psychrotrophic strains. Food Microbiology. 112(2), 120-128.
53. Wooford, N. & David, M. L. (2009). Infections caused by Gram-positive bacteria: a review of global challenge. Infection, 59(1), 4-16
54. Zhang, Y., Liu, X., Jiang, P. & Quek, S. Y. (2015). Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus.