Bioactivities of Hypericum Perforatum L and Equisetum Arvense L fractions obtained with different solvents

Yıl 2019, Cilt: 2 Sayı: 3, 221 - 230, 28.12.2019

Adem Necip , Mesut Işık

https://doi.org/10.38001/ijlsb.636502

Cited By: 13

Öz

Bu çalışmada, Hypericum. Perforatum.L (HPL)
ve Equisetum arvense L. (EAL) den elde edilen HPL sulu ekstraktlarının (HPLAE), HPL metanol ekstraktlarının (HPLME), EAL
sulu ekstraktlarının (EALAE) ve EAL
metanol ekstraktlarının (EALME) antikolinesteraz ve antioksidan etkisi
araştırıldı. Asetilkolinesteraz (AChE) üzerine HPLME fraksiyonu, inhibitör
etkisi gösterirken, diğerleri herhangi bir inhibisyon etkisi göstermemiştir. HPL ve EAL'nin farklı fraksiyonunun
antioksidan aktivitesi, Fe³⁺-Fe²⁺ indirgeme kapasitesi,
ABTS∙+ radikal giderme kapasitesi, DPPH· serbest radikal giderme kapasitesi ve
kuprak metodunu içeren farklı in vitro yöntemler kullanılarak belirlendi.
Çalışmada bu bitki ekstreleri standart antioksidan olan Butil Hidroksi Toluen (BHT),
Butillenmiş hidroksianisol (BHA) ve troloks ile karşılaştırıldı. Bu
bitkilerden elde edilen fraksiyonlar, standartlara yakın %52 radikal giderme
aktivitesine ve orta derecede metal azaltma aktivitesine sahiptir. Sonuç
olarak, oksidatif stresin neden olduğu birçok hastalığı tedavi etmek için
kullanılan bu şifalı bitkilerin farklı fraksiyonları çeşitli biyoaktivitelere
sahiptir.

Anahtar Kelimeler

Anticholinesterase activity, reduction capacity, radical scavenging

Proje Numarası

18240

Kaynakça

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