3-Chloropropionic Acid (3cp) Degradation and Production of Propionic Acid by Newly Isolated Fungus Trichoderma Sp. Mf1

Yıl 2020, Cilt: 3 Sayı: 1, 41 - 50, 15.04.2020

Mohamed Edbeib

https://doi.org/10.38001/ijlsb.677005

Cited By: 3

Öz

Biologically synthesized chemicals have drawn a growing interest recently. The main objectives of the study are to isolate 3CP degrading fungus and to examine its ability to produce Propionic Acid (PA) as a by-product. 3CP is widely used in many pesticide and herbicide synthesis due to biocidal properties but it is toxic and recalcitrant to be removed from the biosphere. Bioremediation approaches through dehalogenation have promised removal of these xenobiotics. Fungi are selected due to its broad enzymatic capacities which is not limited like bacteria. In this research, several fungal isolates have been purified, among the isolates, strain designated as MF1 has shown greater potential in 3CP degradation. Using morphological and molecular approaches, MF1 was identified as Trichoderma sp. Amplification of ITS genome region revealed that the MF1 isolate had 99% identity to Trichoderma asperellum strain AF14 (JX677934.1). Strain MF1 growth rate is 1.42cm/day on solid medium and it was able to produce biomass up to 0.855g/L in liquid minimal medium supplemented with 10mM 3CP. Whereas the growth in control medium containing 1% glucose has resulted in biomass of 1.814g/L. 90.32% of 10mM 3CP were successfully de-chlorinated within 20 days. Confirmed by HPLC, PA was the major product of dehalogenation with highest concentration of 2.72mM at day 10. Presented data can be used for the designing of by-product extraction. Dehalogenation of 3CP by Trichoderma MF1 have not only been successfully removed xenobiotic pollutant but also have open for new strategy on synthesis of industrial required chemicals.

Anahtar Kelimeler

pollutant degradation, dehalogenation, Trichoderma sp., 3- chloropropionic acid, propionic acid synthesis

Kaynakça

• 1. Nemati M., et al., Identification of putative cof-like hydrolase associated with dehalogenase in enterobacter cloacae mn1 isolated from the contaminated sea-side area of the philippines. Malaysian Journal of Microbiology, 2013. 9 (3): p. 253-9.
• 2. Muslem W. H., et al., The potential of a novel β-specific dehalogenase from bacillus cereus wh2 as a bioremediation agent for the removal of β-haloalkanoic acids. Malaysian Journal of Microbiology, 2017. 13 (4): p. 298-307.
• 3. Maldini G. and M. S. Allen Effects of rate and amount of propionic acid infused into the rumen on feeding behavior of holstein cows in the postpartum period. Journal of Dairy Science, 2019. 102 (9): p. 8120-6.
• 4. Lin C., et al., Biodegradation and metabolic pathway of β-chlorinated aliphatic acid in bacillus sp. Cgmcc no. 4196. Applied Microbiology and Biotechnology, 2011. 90 (2): p. 689-96.
• 5. Jing N. H., et al., A further characterization of 3-chloropropionic acid dehalogenase from rhodococcus sp. Hj1. Research Journal of Microbiology, 2008. 3 (6): p. 482-8.
• 6. Mesri S., R. A. Wahab and F. Huyop Degradation of 3-chloropropionic acid (3cp) by pseudomonas sp. B6p isolated from a rice paddy field. Annals of Microbiology, 2009. 59 (3): p. 447-51.
• 7. Hareland W. A., et al., Metabolic function and properties of 4hydroxyphenylacetic acid 1-hydroxylase from pseudomonas acidovorans. Journal of Bacteriology, 1975. 121 (1): p. 272-85.
• 8. Liu D., et al., Rapid mini-preparation of fungal DNA for pcr. Journal of Clinical Microbiology, 2000. 38 (1): p. 471.
• 9. Bergmann J. and J. J. Sanik Determination of trace amounts of chlorine in naphtha. Analytical Chemistry, 1957. 29 (2): p. 241-3.
• 10. Webster J. Introduction to fungi, " cam-bridge university press, cambridge. 169p. 1991. p.
• 11. Sharma P. Fungi and allied organisms: Alpha Science Int'l Ltd.; 2005.
• 12. Parvizpour S., T. Hamid and F. Huyop Molecular identification and biodegradation of 3-chloropropionic acid (3cp) by filamentous fungi-mucor and trichoderma species isolated from utm agricultural land. Malaysian Journal of Microbiology, 2013. 9 (1): p. 120-4.
• 13. Hamzah A., et al., Optimal physical and nutrient parameters for growth of trichoderma virens ukmp-1m for heavy crude oil degradation. Sains Malaysiana, 2012. 41 (1): p. 71-9.
• 14. Purnomo A. S., et al., Bioremediation of ddt contaminated soil using brown-rot fungi. International Biodeterioration & Biodegradation, 2011. 65 (5): p. 691-5.
• 15. Ryu W. R., et al., Biodegradation of pentachlorophenol by white rot fungi under ligninolytic and nonligninolytic conditions. Biotechnology and Bioprocess Engineering, 2000. 5 (3): p. 211-4.
• 16. Haque M., et al., Propionic acid is an alternative to antibiotics in poultry diet. Bangladesh Journal of Animal Science, 2009. 38 (1-2): p. 115-22.