Delphinidin, Luteolin and Halogenated Boroxine Modulate CAT Gene Expression in Cultured Lymphocytes

Yıl 2021, Cilt: 4 Sayı: 1, 25 - 32, 15.04.2021

Nikolina Tomic Maida Hadzic Naida Lojo-kadric , Jasmin Ramic Lejla Pojskic

https://doi.org/10.38001/ijlsb.789990

Cited By: 2

Öz

Luteolin and delphinidin are the flavonoids with known protective roles. They inhibit genotoxic effects induced by halogenated boroxine (HB) in vitro. Statistically significant decrease in the frequency of micronuclei and nuclear buds and suppression of the occurrence of aberrant cells were observed before, but mechanism of its anti-genotoxic activity is still not clear. In our experiment we aimed to quantify HB effects on the relative expression of CAT (catalase) gene and explore antioxidative effects of luteolin and delphinidin via restoration of CAT gene activity. Cell cultures from peripheral blood lymphocytes of five healthy donors were established and treated with independent and concomitant treatments of HB with luteolin or delphinidin. Total RNA was isolated from harvested cells and reverse-transcribed. SYBR based Real-Time PCR amplification method was used. Analysis of results included normalization of ratio of target (CAT) and housekeeping (GAPDH) gene and statistical analysis (REST®). Luteolin itself lead to downregulation of relative CAT gene expression as well as HB. But simultaneous treatment of HB and bioflavonoids lead to upregulation. Delphinidin as independent treatment and as simultaneous treatment caused upregulation of relative CAT gene expression. Obtained results may indicate protective role of delphinidin and luteolin to oxidative damage caused by HB, and also that new approaches to the treatment applications of HB should include bioflavonoids and monitoring corresponding antioxidant system. Our findings indicate that there is a quantifiable effect of luteolin and delphinidine on antioxidant genes which could be used in exact monitoring of oxidative stress related events.

Anahtar Kelimeler

bioflavonoids, dipotassium-trioxohydroxytetrafluorotriborate, catalase, antioxidants

Kaynakça

• 1. Sies, H., Oxidative stress: a concept in redox biology and medicine. Redox biology, 2015. 4: p. 180–183.
• 2. Pisoschi, AM. and A. Pop, The role of antioxidants in the chemistry of oxidative stress: A review. European Journal of Medicinal Chemistry, 2015. 97: p. 55–74.
• 3. Ghezzi, P., et al., The oxidative stress theory of disease: levels of evidence and epistemological aspects. British Journal of Pharmacology, 2017. 174: p. 1784–1796.
• 4. Kodydková, J., et al., Human Catalase, Its Polymorphisms, Regulation and Changes of Its Activity in Different Diseases. Folia Biologica, 2014. 60: p. 153-167.
• 5. Gao, G., et al., Luteolin exhibits anti-breast cancer property through up-regulating miR-203. Artificial Cells, Nanomedicine, and Biotechnology, 2019. 47 (1): p. 3265-3271.
• 6. Imran, M., et al., Luteolin, a flavonoid, as an anticancer agent: A review. Biomedicine & Pharmacotherapy, 2019. 112: 108612.
• 7. Seelinger, G., et al., Anti-carcinogenic Effects of the Flavonoid Luteolin. Molecules, 2008. 13: p. 2628-2651.
• 8. Lin, Y., et al., Luteolin, a flavonoid with potentials for cancer prevention and therapy. Current Cancer Drug Targets, 2008. 8 (7): p. 634–646.
• 9. Murata, M., et al., Delphinidin Prevents Muscle Atrophy and Upregulates miR-23a Expression. Journal of Agricultural and Food Chemistry, 2017. 65 (1): p. 45-50.
• 10. Kuo, H.D., et alAnthocyanin Delphinidin Prevents Neoplastic Transformation of Mouse Skin JB6 P+ Cells: Epigenetic Re-activation of Nrf2-ARE Pathway. The AAPS Journal, 2019. 21 (5): p. 83.
• 11. Lee, D.Y., et al., Cytotoxic effects of delphinidin in human osteosarcoma cells. Acta Orthopaedica et Traumatologica Turcica, 2018. 52 (1): p. 58–64.
• 12. Ni, T., W. Yang, and Y. Xing, Protective effects of delphinidin against H2O2 induced oxidative injuries in human retinal pigment epithelial cells. Bioscience Reports, 2019. BSR20190689.
• 13. Galic, B., Removal of skin changes. European patent EP1996514, 2013.
• 14. Haverić, S., et al., Effects of dipotassium trioxohydroxytetrafluorotriborate (K2[B3O3F4OH]) on genetic material and inhibition of cell division in human cell cultures. Drug and Chemical Toxicology, 2011. 34 (3): p. 250-254.
• 15. Haveric, S., et al., Genotoxicity evaluation of dipotassium-trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), in human lymphocyte cultures and mice reticulocytes. Brazilian Archives of Biology and Technology, 2016. 59: e16160195.
• 16. Marasović, M., et al., Quantum Chemical and Biochemical Study on Antioxidant Properties of Halogenated Boroxine K2[B3O3F4OH]. Croatica chemica acta, 2017. 90 (2): p. 155–161.
• 17. Islamovic, S., B. Galic, and M. Milos, A study of the inhibition of catalase by dipotassium-trioxohydroxytetrafluorotriborat K2[B3O3F4OH]. Journal of Enzyme Inhibition and Medicinal Chemistry, 2014. 29 (5): p. 744–748.
• 18. Hadzic, M., et al., Inhibitory effects of delphinidin and luteolin on genotoxicity induced by K2(B3O3F4OH) in human lymphocytes in vitro. Biologia, 2015. 70: p. 553–8.
• 19. Hadzic, M., et al., Bioflavonoids protect cells against halogenated boroxine-induced genotoxic damage by upregulation of hTERT expression. Zeitschrift fur Naturforschung. C, Journal of biosciences, .2018. 74 (5-6): p. 125-129.
• 20. Ryss, I.G., and M.M. Slutskaya, Report on the Platinum Sector. Akademia Nauka SSSR, 1951. 26: p. 216.
• 21. Pfaffl, M.W., G.W. Horgan, and L. Dempfle, Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research, 2002. 30 (9): e36.
• 22. Luca, V.S., A. Miron, and A.C. Aprotosoaie, The antigenotoxic potential of dietary flavonoids. Phytochemistry Reviews, 2016. 15 (4): p. 591-625.
• 23. Ezić, J., et al., Analysis of delphinidin and luteolin genotoxicity in human lymphocyte culture. Journal of Health Sciences, 2015. 5 (2): p. 41-45.
• 24. Azevedo, L., et al., Differential response related to genotoxicity between eggplant (Solanum melanogena) skin aqueous extract and its main purified anthocyanin (delphinidin) in vivo. Food and Chemical Toxicology, 2007. 45: p. 5852-5858.
• 25. Lazzé, M.C., et al., Anthocyanins induce cell cycle perturbations and apoptosis in different human cell lines. Carcinogenesis, 2004. 25: p. 81427-81433.
• 26. Hadžić, M., Analysis of potential of bioflavonoids (luteolin and delphinidin) in inhibition of genotoxic and cytotoxic effects of halogenated boroxine in vitro. MSc Thesis, University of Sarajevo, 2013.
• 27. Chakraborthy, A., et al., Antioxidant and pro-oxidant activity of Vitamin C in oral environment. Indian Journal of Dental Research, 2014. 25 (4): p. 499.
• 28. Chobot, V., and F. Hadacek, Exploration of pro-oxidant and antioxidant activities of the flavonoid myricetin. Redox report Communications in free radical research, 2011. 16 (6): p. 242–247.
• 29. Wang, J., et al., Antioxidant and Pro-Oxidant Activities of Melatonin in the Presence of Copper and Polyphenols In Vitro and In Vivo. Cells, 2019. 8 (8): p. 903.
• 30. Ribeiro, D., et al., Antioxidant and pro-oxidant activities of carotenoids and their oxidation products. Food and Chemical Toxicology, 2018. 120: p. 681-699.