Fish Immunity Against Nervous Necrosis Virus (NNV) Infection: A Review

Yıl 2025, Cilt: 8 Sayı: 1, 74 - 84, 15.04.2025

Irfan Hakimi Roslan , Azila Abdullah , Nur Nazifah Mansor , Mohd Firdaus Nawi , Nur Amalin Sofea Abdul Rahim , Hazreen Nita Mohd Khalid

https://doi.org/10.38001/ijlsb.1542126

Öz

Fish are unique organisms fitted with a certain degree of immune system that are similarly to mammals, made up of innate and adaptive immunity. Both innate and adaptive immunity mechanisms in fish are crucial as the first line of defense as well as secreting antibodies and memory cells following an infection. Innate immunity comprised of non-specific cellular and the nonspecific humoral components while adaptive immunity consists of specialized systemic cells and mechanisms that are categorized into two main groups: the humoral and cellular components. Prior to Nervous Necrosis Virus (NNV) infection caused by small (25-30 nm), non-enveloped and icosahedral shaped virus belongs to genus Betanodavirus, both innate and adaptive immunity mechanisms have their own parts to play. As Nervous Necrosis Virus (NNV) invades fish body, pathogen will be break down through phagocytosis followed by presentation of small protein fragments on MHC class II molecules. This will eventually activate adaptive immunity pathway to stimulates the proliferation and maturation of the B cells. As B cells matured, antibodies will be produced to suppress the pathogen through series of process known as opsonization and neutralization before phagocytosis takes place. Following secondary exposure with the same antigen, B cells will rapidly proliferate, releasing significant number of antibodies to clear the antigen.

Anahtar Kelimeler

Immunity , Viral Nervous Necrosis (VNN) , Nervous Necrosis Virus (NNV) , Betanodavirus , Antibody , Antigen

Etik Beyan

All animal procedures were conducted in accordance with the guidelines set by the Institution's Animal Care and Use Committee (IACUC) (ID: IACUC 2023-003). The protocol was approved by the Committee on the Ethics of Animal Experiments of the International Islamic University Malaysia. All sampling was performed under anaesthesia, and all efforts were made to minimize suffering.

Destekleyen Kurum

National Fish Health Research Centre, Department of Fisheries Malaysia

Proje Numarası

SPG23-078-0078 and SPG24-116-0116

Teşekkür

I would like to express my appreciation to National Fish Health Research Institute, for providing the fund for this project and Kulliyah of Science, IIUM for their support throughout this project. I also would like to extend my utmost appreciation and gratitude to my project supervisors; my colleague and laboratory staff of Kulliyah of Science, IIUM for their supervision, support and all necessary materials for this project

Fish Immunity Against Nervous Necrosis Virus (NNV) Infection: A Review

Öz

Fish are unique organisms fitted with a certain degree of immune system that are similarly to mammals, made up of innate and adaptive immunity. Both innate and adaptive immunity mechanisms in fish are crucial as the first line of defense as well as secreting antibodies and memory cells following an infection. Innate immunity comprised of non-specific cellular and the nonspecific humoral components while adaptive immunity consists of specialized systemic cells and mechanisms that are categorized into two main groups: the humoral and cellular components. Prior to Nervous Necrosis Virus (NNV) infection caused by small (25-30 nm), non-enveloped and icosahedral shaped virus belongs to genus Betanodavirus, both innate and adaptive immunity mechanisms have their own parts to play. As Nervous Necrosis
Virus (NNV) invades fish body, pathogen will be break down through phagocytosis followed by presentation of small protein fragments on MHC class II molecules. This will eventually activate adaptive immunity pathway to stimulates the proliferation and maturation of the B cells. As B cells matured, antibodies will be produced to suppress the pathogen through series of process known as opsonization and neutralization before phagocytosis takes place. Following secondary exposure with the same antigen, B cells will rapidly proliferate, releasing significant number of antibodies to clear the antigen.

Anahtar Kelimeler

Immunity , Viral Nervous Necrosis (VNN) , Nervous Necrosis Virus (NNV) , Betanodavirus , Antibody , Antigen

Etik Beyan

All animal procedures were conducted in accordance with the guidelines set by the Institution's Animal Care and Use Committee (IACUC) (ID: IACUC 2023-003). The protocol was approved by the Committee on the Ethics of Animal Experiments of the International Islamic University Malaysia. All sampling was performed under anaesthesia, and all efforts were made to minimize suffering.

Destekleyen Kurum

National Fish Health Research Centre, Department of Fisheries Malaysia

Proje Numarası

SPG23-078-0078 and SPG24-116-0116

Teşekkür

I would like to express my appreciation to National Fish Health Research Institute, for providing the fund for this project and Kulliyah of Science, IIUM for their support throughout this project. I also would like to extend my utmost appreciation and gratitude to my project supervisors; my colleague and laboratory staff of Kulliyah of Science, IIUM for their supervision, support and all necessary materials for this project

Kaynakça

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