Enzymatic Saccharaification of Bagasse: Effects of Different Pre-Treatment Methods

Yıl 2013, Cilt: 3 Sayı: 2, 230 - 234, 01.06.2013

Md. Zahangir Alam Md. Moniruzzaman SMA Sujan Mosharof Hossain Mohammad Shah Jamal

Öz

About 800,000 tons of bagasse is obtained per year as a by-product from sugar industries in Bangladesh. Bagasse is one of the most readily available lignocellulosic biomass that is a potential source for ethanol production through enzymatic saccharification and hydrolysis. Pretreatment enhances saccharification by removing lignin and hemicellulose. In this study pretreatment of bagasse was done using hot compressed water (HCW), (HCW+H3PO4) and (HCW+NH3). Effects of different pretreatment parameters such as temperature, residence time, concentration of NH3 and H3PO4 were studied in detail. (HCW+H3PO4) system was found to be more effective compared to other systems investigated in this study. A maximum yield of glucose (60%) and xylose (71%) was obtained in case of (HCW+H3PO4) system at temperature180°C and 160°C and residence time of 30 min and 15 min, respectively.

Anahtar Kelimeler

Saccharification, Bagasse, Biofuel, Pretreatment, Hot compressed water.

Kaynakça

• C. E. Wyman, “Bio-mass Ethanol: Technical Progress, Opportunities, and Commercial Challenges”, Annu. Rev. Energy Environ. Vol. 24, pp. 189-226, November 1999.
• S. C. Yat, A. Berger and D. R. Shonnard, “Kinetic Characterization for dilute sulfuric acid hydrolysis of timber varieties and switchgrass”, Bioresour Technol. Vol. 99, pp. 3855-3863, June 2008.
• B. C. Saha, “Hemicellulose bioconversion”, J. Ind. Microbiol. Biotechnol. Vol. 30, pp. 279-291, May 2003.
• Y. Sun and J. Cheng, “Hydrolysis of lignocellulosic materials for ethanol production: A Review”, Bioresource. Technol., vol. 83, pp. 1-11, May 2002.
• S. Pareek, J. Azuma, Y. Shimizu and S. Matsui, “Hydrolysis of newspaper polysaccharides under sulfate reducing Biodegradation, vol. 11, pp. 229-237, 2000. producing conditions”,
• C. M. Roche, C. J. Dibble and J. J. Stickel, “Laboratory- scale lignocellulosic Biotechnol. Biofuels, vol. 2, pp. 28 (1-11), November at biomass of high-solids loadings”, G. Brodeur, E. Yau, K. Badal, J. Collier, K. B. Ramachandran and S. Ramakrishnan, “Chemical and Physicochemical Biomass: A Review” Enzyme Research, doi: 4061/2011/787532, January 2011. Lignocellulosic
• S. Beszedes, M. Abel, G. Szabo, Z. Laszlo, G. Szabo and C. Hodur, “Application of response surface methodology to optimize microwave sludge conditioning for enhanced biogas production”, Annals of Faculty Engineering Hunedoara, International Journal of Engineering, vol. IX, pp. 189-193, 2011.
• M. Irfan, M. Gulsher, S. Abbas, Q. Syed, M. Nadeem and S. Baig, “Effect of various pretreatment conditions on enzymatic saccharification”, Songklanakarin J. Sci. Technol. 33, 397-404, Jul.-Aug. 2011.
• D. L. Yin, Y. Jing, W. W. AlDajani, S. D. Ulrike Tschirner, J. Schilling and R. J. Kazlauskas, “Improved pretreatment enzymatically-generated BioresourTechnol., Vol. 102, 5183-5192, 2011. using acid”, peracetic
• A. Sluiter, B. Hames, R. Ruiz, C. Scarlata, J. Sluiter, D. Templeton and D. Crocker, Determination of Structural Carbohydrates and Lignin in Biomass. Laboratory Analytical Procedure, National Renewable Energy Laboratory Golden Co., July 2006.
• A. Sluiter, B. Hames, R. Ruiz, C. Scarlata, J. Sluiter and D. Templeton, Determination of Sugars, Byproducts, and Degradation Products in Liquid Fraction Process Samples. Laboratory Analytical Procedure, National Renewable Energy Laboratory Golden Co., Aug. 2006.
• D. J. Fox, P. P. Gray, N. W. Dunn and W. L. Marsden, An Explanation of Discrepancy between the Results of HPLC and DNS Assays in the Analysis of Lignocellulosic Biotechnol., vol. 34, pp. 171-175, September 1984. J. Chem. Technol.
• Z. Wu and Y. Y. Lee, “Ammonia recycled percolation as a complementary pretreatment to the dilute acid process”, Appl. Biochem. Biotech., vol. 63-65, pp. 34, 1997.
• K. Boominathan and C. A. Reddy, “cAMP-mediated differential regulation of lignion peroxidase and manganese dependent peroxidase production in the white-rot basidiomycete Phanerochaete chrysosporium”, Proc. Natl. Acad. Sci. USA, vol. 89, pp. 5586-5590, June
• T. K. Kirk and R. L. Farrell, “Enzymatic "combustion": the microbial degradation of lignin”, Annu. Rev. Microbiol., vol. 41, pp. 465-5-5, 1987.
• M. S. Jamal, S. M. A. Sujan, M. Y. Miah, S.K.Banik, S. U. Ahmed and B. Feroza, “Ball Milling Pretreatment of Bagasse for Ethanol Production by Enzymatic Saccharification and Fermentation”, J. Sci. Ind. Res, vol. 46, pp. 353-358, 2011.
• L. R. Lynd, J. H. Cushman, R. J. Nichols and C. E. Wyman, “Fuel ethanol from cellulosic biomass”, Science, vol. 251, pp. 1318-1323, March 1991.
• A. Demirbas, “Biofuels securing the planet's future energy needs”, Energy Conversion and Management, vol. , pp. 2239-2249, September 2009.