Biosynthesis and Characterization of Copper Oxide Nanoparticles using Cimin Grape (Vitis vinifera cv.) Extract

Öz

Nowadays, nanoparticle synthesis has been a very important research area because of the wide use of nanoparticles in many fields. Green synthesis is one step ahead of other synthesis methods due to both cost reduction in production and environmentally friendly approach. For these reasons, we chose green synthesis method which is nature friendly in our research. In this study, it was aimed synthesis of copper nanoparticles by the green synthesis method using the water extract of Erzincan Cimin grape (Vitis vinifera cv. Black plum) from 0.1 M CuCl2.2H2O in the reaction medium. Then, optimum conditions for the green synthesis reaction had been determined and product optimization of copper nanoparticles was made. Optimum conditions for synthesis were measured at a wavelength of 355 nm (for CuO nanoparticles). It was observed that the best synthesis reaction was occurred at pH 5, temperature 60 °C, 0.05 M metal ion concentration and within the 60 minutes.The characterization of the CuO nanoparticles was obtained then was characterized using spectroscopic techniques such as UV-VIS, FTIR, XRD and SEM. When the chromatograms obtained using these techniques were examined, it was understood that 25-50 nm morphologically homogeneous nanoparticles were synthesized. Due to the new physical and chemical properties of obtained copper nanoparticles using a cost-effective and environmentally friendly green synthesis method using water extract of Erzincan Cimin Grape; it was thought that in many areas (optical, biosensors, etc.) could be used.

Anahtar Kelimeler

• Copper(II)oxide NPs
• Green synthesis
• Nanotechnology

Biosynthesis and Characterization of Copper Oxide Nanoparticles using Cimin Grape (Vitis vinifera cv.) Extract

Abstract

Nowadays, nanoparticle synthesis has been a very important research area because of the wide use of nanoparticles in many fields. Green synthesis is one step ahead of other synthesis methods due to both cost reduction in production and environmentally friendly approach. For these reasons, we chose green synthesis method which is nature friendly in our research. In this study, it was aimed synthesis of copper nanoparticles by the green synthesis method using the water extract of Erzincan Cimin grape (Vitis vinifera cv. Black plum) from 0.1 M CuCl₂.2H₂O in the reaction medium. Then, optimum conditions for the green synthesis reaction had been determined and product optimization of copper nanoparticles was made. Optimum conditions for synthesis were measured at a wavelength of 355 nm (for CuO nanoparticles). It was observed that the best synthesis reaction was occurred at pH 5, temperature 60 °C, 0.05 M metal ion concentration and within the 60 minutes.The characterization of the CuO nanoparticles was obtained then was characterized using spectroscopic techniques such as UV-VIS, FTIR, XRD and SEM. When the chromatograms obtained using these techniques were examined, it was understood that 25-50 nm morphologically homogeneous nanoparticles were synthesized. Due to the new physical and chemical properties of obtained copper nanoparticles using a cost-effective and environmentally friendly green synthesis method using water extract of Erzincan Cimin Grape; it was thought that in many areas (optical, biosensors, etc.) could be used.

Keywords

• Copper(II)oxide NPs
• Green synthesis
• Nanotechnology

References

1. Bhusan, S. H., Kumar, A. A., Ashish, T. F., & Lal, K. M. (2012). Evaluation of Polyherbal formulation for diuretic activity in albino rats. Asian Pacific Journal of Tropical Disease, 2, S442-S445.
2. Fan, P., & Lou, H. (2004). Effects of polyphenols from grape seeds on oxidative damage to cellular DNA. Molecular and cellular biochemistry, 267(1-2), 67-74.
3. Ismail, E. H., Khalil, M. M., Al Seif, F. A., & El-Magdoub, F. (2014). Biosynthesis of gold nanoparticles using extract of grape (Vitis vinifera) leaves and seeds. Prog Nanotechnol Nanomater, 3, 1-12.
4. Singh, P. P., & Bhakat, C. (2012). Green synthesis of gold nanoparticles and silver nanoparticles from leaves and bark of Ficus carica for nanotechnological applications. Int. J. Sci. Res. Pub, 2(5), 1-4.
5. Konishi, Y., Ohno, K., Saitoh, N., Nomura, T., Nagamine, S., Hishida, H., ... & Uruga, T. (2007). Bioreductive deposition of platinum nanoparticles on the bacterium Shewanella algae. Journal of biotechnology, 128(3), 648-653.
6. Salam, H. A., Sivaraj, R., & Venckatesh, R. (2014). Green synthesis and characterization of zinc oxide nanoparticles from Ocimum basilicum L. var. purpurascens Benth.-Lamiaceae leaf extract. Materials Letters, 131, 16-18.
7. Liu, F. K., Huang, P. W., Chang, Y. C., Ko, F. H., & Chu, T. C. (2004). Microwave-assisted synthesis of silver nanorods. Journal of materials research, 19(2), 469-473.
8. Singh, A., Jain, D., Upadhyay, M. K., Khandelwal, N., & Verma, H. N. (2010). Green synthesis of silver nanoparticles using Argemone mexicana leaf extract and evaluation of their antimicrobial activities. Dig J Nanomater Bios, 5(2), 483-489.

9. Suramwar, N. V., Thakare, S. R., & Khaty, N. T. (2013). One-Pot Green Synthesis of Ni Nanoparticles and Study of Its Catalytic Activity in the Hydrothermal Reduction of p-Nitrophenol. Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, 43(1), 57-62.
10. Park, B. K., Jeong, S., Kim, D., Moon, J., Lim, S., & Kim, J. S. (2007). Synthesis and size control of monodisperse copper nanoparticles by polyol method. Journal of colloid and interface science, 311(2), 417-424.
11. Han, W. K., Choi, J. W., Hwang, G. H., Hong, S. J., Lee, J. S., & Kang, S. G. (2006). Fabrication of Cu nano particles by direct electrochemical reduction from CuO nano particles. Applied Surface Science, 252(8), 2832-2838.
12. Zhu, H. T., Zhang, C. Y., & Yin, Y. S. (2004). Rapid synthesis of copper nanoparticles by sodium hypophosphite reduction in ethylene glycol under microwave irradiation. Journal of Crystal Growth, 270(3), 722-728.
13. Castangia, I., Marongiu, F., Manca, M. L., Pompei, R., Angius, F., Ardu, A., ... & Ennas, G. (2017). Combination of grape extract-silver nanoparticles and liposomes: A totally green approach. European Journal of Pharmaceutical Sciences, 97, 62-69.
14. Cicek, S., Gungor, A. A., Adiguzel, A., & Nadaroglu, H. (2015). Biochemical evaluation and green synthesis of nano silver using peroxidase from Euphorbia (Euphorbia amygdaloides) and its antibacterial activity. Journal of Chemistry, 2015.
15. Gültekin, D. D., Güngör, A. A., Önem, H., Babagil, A., & Nadaroğlu, H. (2016). Synthesis of Copper Nanoparticles Using a Different Method: Determination of Its Antioxidant and Antimicrobial Activity. Journal of the Turkish Chemical Society, Section A: Chemistry, 3(3), 623-636.
16. Lanje, A. S., Sharma, S. J., Pode, R. B., & Ningthoujam, R. S. (2010). Synthesis and optical characterization of copper oxide nanoparticles. Adv Appl Sci Res, 1(2), 36-40.
17. Azam, A., Ahmed, A. S., Oves, M., Khan, M. S., & Memic, A. (2012). Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and-negative bacterial strains. International Journal of Nanomedicine, 7, 3527.
18. Saif, S., Tahir, A., Asim, T., & Chen, Y. (2016). Plant mediated green synthesis of CuO nanoparticles: comparison of toxicity of engineered and plant mediated CuO nanoparticles towards Daphnia magna. Nanomaterials, 6(11), 205.