In vitro Propagation Techniques for Some Geophyte Ornamental Plants with High Economic Value

Yıl 2015, Cilt: 2 Sayı: 1, 27 - 49, 14.01.2015

Arzu Çığ Gülçinay Başdoğan

Öz

Propagation of some ornamental plants has increased tremendously due to the demand for them as cut flowers, in addition to their usage for interior and exterior landscaping purposes. Geophytes (bulbous-tuberous) are the most preferred group among the ornamental plants due to their aesthetic features, suitability to be cut flowers and their fragrance. These plants are highly propagated and consumed. The geophyte species which are highly profitable, globally traded and constituting 90% of the flower bulb market are Tulipa (tulip), Lilium (lily), Narcissus (daffodil), Gladiolus (gladioli), Iris (iris) and Hyacinthus (hyacinth). In vitro propagation techniques, which provide disease-free mass production options, have started to be used increasingly to fulfil the demand for these species in the market. In this study, the results of in vitro propagation studies for some economically valuable tulip, lily, daffodil, gladiolus, iris and hyacinth species are provided.

Anahtar Kelimeler

Propagation, Geophyte, In vitro, Ornamental Plant

Kaynakça

• Zhen-guang, C., 1982.
• Nutrient medium+active charcoal
• active carbon in basic medium
• Pifang, Z. et al., 1985b. N. chinensis
• The white compact callus was initiated from scale segments with
• the basal plate on MS+1 mg l-1 BA and 0.1 mg l-1 2,4-D.
• Adventitious shoots were produced from callus cultures by
• transferring them on MS or with 1 mg l-1 BA or containing 0.1-0.5
• NAA. Most of bulblets developed leaves and roots ½ MS+0.01-0.1 mg l NAA or without any growth substance, particularly with 0.03 mg l-1 NAA.
• Hengsen, G. and Cuihua, G., 1987. N. chinensis
• MS+different doses of PGR
• increased adventitious shoots on MS+0.1 mg l-1 NAA.
• Hengsen, G. et al., 1987. N. chinensis MS+0-5 mg l-1 BA, 0-1 mg l induced bulbils. The percentage of induction reached 70%.
• Yimin, H. and Guoning, Q., 1991. N. chinensis MS+NAA and 6-BA
• The differentiation rate of puff callus induced by low concentration
• of NAA (0. 5 mg l)+6 BA (1-8 mg l-1) is low.
• Weilian, H. et al., 1993. Narcissus c.v.s
• “St. Keverne” and “hawera” MS+different doses of PGR
• BA in single leaf cultures than in shoot clump cultures. NAA
• stimulated bulbil formation on MS+176 mM sucrose for both
• cultivars. “St Keverne” showed good bulbil development with 0.54
• μM NAA, 5.4 μM IAA and 5.4 μM lBA and “Hawera” responded only to 27 μM IAA.
• Staikidou, I. et al., 1994.
• N. bulbocodium Twin scales PGR
• mg l-1) resulted in shoot initiation and leaf development. Tiny bulbs
• were obtained with MS+BAP+IBA for a long period (70 days). The
• final size of the bulbs was not increased by the presence of NAA
• butlincrease but a better root system was developed by it.
• Santos, J. et al., 1998.
• N. pseudonarcissus Leaves, bulbs and c.v.s
• harvest” and “St. Keverne” MS+different doses of A range of 2,4-D and BAP concentrations started embryogenesis. 5 μM 2,4-D and 0.5 μM or 5 μM BAP was more efficieny on somatic
• embryogenesis (SEs) than other combinations. SEs were produced
• on scape explants earlier. SEs converted to plantlets with 4.9 μM IBA.
• Sage, D.O. et al., 2000. N. chinensis PGR
• mg l-1 2,4-D. The calli could differentiate on the media added BA
• and NAA. But the root differentiation rate could be increased, and
• the differentiation rate of buds decreased with increasing of NAA
• concentration in the media. The buds could grow up to small plants
• on the MS+BA and NAA. Yu, W., 2001.
• Narcissus c.v. “pink Young leaves charm”
• MS+different doses of PGR
• medium for differentiation of rosette bud. For proliferation: MS+1.5
• BA +1.0 mg lNAA and for induction of roots: ½ MS+0.2 mg l-1 6-BA+0.5 mg lNAA.
• Zhu, H. et al., 2007. Narcissus “fortissimo” ½ MS+different doses of
• The root of bulblets grew strongly on ½ MS+0.1 mg l-1 NAA or 0.1
• mg l-1 IBA. The root was shorter and slimmer on ½ MS+0.05 mg l
• IBA. The bulblets grew faster on ½ MS+1.0 mg l-1 NAA or 0.5 mg
• l-1 IBA. ½ MS+0.1 mg l-1 NAA or 0.1 mg l-1 IBA had the significant
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• The best medium was MS+3.2 mg l-1 6-BA+.02 mg l-1 NAA for
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• MS+different doses of PGR
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• NAA, 2.0 mg lactivated charcoal and sucrose 60.0 g·l or MS+
• 0 mg l-16-BA, 1.0 mg l-12,4-D, 2.0 mg l-1 activated charcoal and
• 0 g·l-1sucrose; for roots: MS +1.0 mg l-1 6-BA, 0.5 mg l-1 2,4-D,
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• Twin-scale with basal plate was more suitable explant was
• described. The more appropriate medium for primary culture was
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• proliferation medium was MS+1.5 mg l-1 6-BA+0.3 mg l-1 NAA, its
• induction rate was 668%.The rooting rate of the bulblets was 80%
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• the capacity to induce colorless embryogenic calli. Production of
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