Research Article
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Year 2021, Volume: 02 Issue: 02, 95 - 100, 16.08.2021
https://doi.org/10.53811/ijtcmr.893132

Abstract

References

  • 1. Chassaing B, Koren O, Goodrich J K, Poole A C, Srinivasan S, Ley R E, Gewirtz A T. Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome. Nature, 2015; 519(7541), 92.
  • 2. Goulet O. “Potential role of the intestinal microbiota in programming health and disease”, Nutrition Reviews, 2015; 73(suppl_1), 32-40.
  • 3. Roquetto A R, Monteiro N E S, Moura C S, Toreti V C, de Pace F, dos Santos A, ... & Amaya-Farfan J. “Green propolis modulates gut microbiota, reduces endotoxemia and expression of TLR4 pathway in mice fed a high-fat diet”, Food Research International, 2015; 76, 796-803.
  • 4. Huang Y, Shi X, Li Z, Shen Y, Shi X, Wang L, .. & Zhao L. “Possible association of Firmicutes in the gut microbiota of patients with major depressive disorder”, Neuropsychiatric Disease and Treatment, 2018; 14, 3329.
  • 5. Louis P, Hold G L, Flint H J. “The gut microbiota, bacterial metabolites and colorectal cancer”, Nature Reviews Microbiology, 2014;12(10), 661.
  • 6. Carding S, Verbeke K, Vipond D T, Corfe B M, Owen L J. “Dysbiosis of the gut microbiota in disease”, Microbial Ecology in Health and Disease, 2015; 26(1), 26191.
  • 7. Edalati E, Saneei B, Alizadeh M, Hosseini S S, Bialvaei A Z, Taheri K. Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning. New microbes and new infections, 2019; 27, 64-68.
  • 8. de Paula B P, Chávez D W H, Lemos Junior W J F, Guerra A. F, Corrêa M F D, Pereira K S, Coelho, M A Z. Growth parameters and survivability of Saccharomyces boulardii for probiotic alcoholic beverages development. Frontiers in microbiology, 2019 ; 10, 2092.
  • 9. Pais P, Almeida V, Yılmaz M, Teixeira M C. Saccharomyces boulardii: What makes it tick as successful probiotic?. Journal of Fungi, 2020; 6(2), 78.
  • 10. Boynukara B, Gülhan T, Develi Z Ş. Veteriner Hekimlikte Probiyotik Kullanimi. Doğanın Sesi, 2018 ; (1), 43-48.
  • 11. Fijan, S. (2016). Antimicrobial effect of probiotics against common pathogens. In Tech, Venkateswera.
  • 12. Ağyar Yoldaş P , Yoldaş T , Sipahi N . Cytotoxicity of Some Retail Food Supplements. International Journal of Traditional and Complementary Medicine Research. 2020; 1(3): 131-136.
  • 13. Karimi, S., Rashidian, E., Birjandi, M., & Mahmoodnia, L. Antagonistic effect of isolated probiotic bacteria from natural sources against intestinal Escherichia coli pathotypes. Electronic physician, 2018;10(3), 6534.
  • 14. Vaseeharan, B. A. R. P., & Ramasamy, P. Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Letters in applied microbiology, 2003; 36(2), 83-87.
  • 15. Sanders M E, Levy D D. The science and regulations of probiotic food and supplement product labeling. Annals of the New York Academy of Sciences, 2011;1219, E1-E23.
  • 16. AdebayoTayo, B., & Akpeji, S. Probiotic viability, physicochemical and sensory properties of probiotic pineapple juice. Fermentation, 2016; 2(4), 20.
  • 17. de Simone C. The unregulated probiotic market. Clinical Gastroenterology and Hepatology, 2019; 17(5), 809-817.
  • 18. Clinical and Laboratory Standards Institute. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria. The 3rd Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2016.
  • 19. Valdez R M A, ann Ximenez-Fyvie L, Caiaffa K S, Dos Santos V R, Cervantes R M G, Almaguer-Flores A, Duque C. Antagonist effect of probiotic bifidobacteria on biofilms of pathogens associated with periodontal disease. Microbial Pathogenesis, 2021; 150, 104657.
  • 20. Çelik E Ş, Ergün S, Yilmaz S. Bacillus subtilis ve Lactobacillus plantarum Probiyotik Bakterilerinin Bazı Balık Patojenleri Üzerine in vitro Antagonistik Etkisi. Journal of Anatolian Environmental and Animal Sciences, 2019; 4(2), 278-284.
  • 21. Kechagia M, Basoulis D, Konstantopoulou S, Dimitriadi D, Gyftopoulou K, Skarmoutsou N, Fakiri E. M. Health benefits of probiotics: a review. International Scholarly Research Notices, 2013:doi.org/10.5402/2013/481651.
  • 22. Nacakoğlu İ, Sipahi N , Aydın M , Kaya E. Medicinal Plants Meeting with Mud: Phyto-Peloid. International Journal of Traditional and Complementary Medicine Research, 2020; 1(1): 33-41.
  • 23. Kolacek S, Hojsak I, Canani R B, Guarino A, Indrio F, Pot B, ... & Weizman Z. Commercial probiotic products: a call for improved quality control. A position paper by the ESPGHAN Working Group for Probiotics and Prebiotics. Journal of pediatric gastroenterology and nutrition, 2017; 65(1):117-124.
  • 24. Özer M, Özyurt G, Harsa, ŞT.. Probiyotik ve prebiyotiklerin bağırsak-beyin aksına etkisi. Akademik Gıda, 2019;17(2):269-280.
  • 25. Mollakhalili MN, Mortazavian A M, Sohrabvandi S, Cruz AGD & Mohammadi R. Probiotic supplements and food products: comparison for different targets. Applied Food Biotechnology, 2017;4(3): 123-132.
  • 26. Wang Y, Jiang Y, Deng Y, Yi C, Wang Y, Ding M, ... & Wong A. Probiotic Supplements: Hope or Hype?. Frontiers in Microbiology, 2020;11, 160.
  • 27. Iglesias M B, Abadias M, Anguera M, Sabata J, & Vi I. Antagonistic effect of probiotic bacteria against foodborne pathogens on fresh-cut pear. LWT-Food Science and Technology, 2017; 81, 243-249.
  • 28. Corr S C, Gahan C G, & Hill C. Impact of selected Lactobacillus and Bifidobacterium species on Listeria monocytogenes infection and the mucosal immune response. FEMS Immunology & Medical Microbiology, 2007; 50(3), 380-388.
  • 29. Maragkoudakis P A, Zoumpopoulou G, Miaris C, Kalantzopoulos G, Pot B, Tsakalidou E. Probiotic potential of Lactobacillus strains isolated from dairy products. International Dairy Journal, 2006; 16(3), 189-199.
  • 30. Guo S, Li L, Xu B, Li M, Zeng Q, Xiao H, ... & Zhang, G. A simple and novel fecal biomarker for colorectal cancer: ratio of Fusobacterium nucleatum to probiotics populations, based on their antagonistic effect. Clinical chemistry, 2018; 64(9), 1327-1337.
  • 31. Gudisa A. In vitro antagonistic effect of probiotic LAB isolated from traditional fermented products against some antibiotics resistant food borne pathogens 2021. (Doctoral dissertation).
  • 32. O’Connor P M, Kuniyoshi T M, Oliveira R P, Hill C, Ross R P, & Cotter P. D. Antimicrobials for food and feed; a bacteriocin perspective. Current opinion in biotechnology, 2020; 61, 160-167.
  • 33. Yu H S, Lee N K, Choi A J, Choe J S, Bae C H, Paik H D. Antagonistic and antioxidant effect of probiotic Weissella cibaria JW15. Food science and biotechnology, 2019; 28(3), 851-855.

Investigation of Antagonistic Effect of Probiotic Food Supplement against Different Strains of Bacteria

Year 2021, Volume: 02 Issue: 02, 95 - 100, 16.08.2021
https://doi.org/10.53811/ijtcmr.893132

Abstract

Objective: Probiotics are an important part of the microbiota associated with many diseases. The production of food and food supplements containing probiotic products has increased considerably in recent years, due to their protective effect on health and antagonistic effect against some pathogenic microorganisms. However, it is unknown whether the content of these products has the expected effect. In this study was aimed to investigate the antagonistic effect of Probiotics & Prebiotics® commercial product.
Material-Method: The probiotic food supplement was obtained from Aym-Net®. Antagonistic effect of the product on 10 different test bacteria was investigated by the agar spot assay method. Also lyophilized probiotics were cultured in MRS broth medium and centrifuged at 3500 rpm for 25 minutes in order to obtain metabolites that the probiotic microorganisms provide their effectiveness. Effect of probiotic supernatant on test bacteria were determined to disk diffusion test by CLSI. Each test bacteria combined with the probiotic product incubated at 37⸰C for 24 hours to determine the bacterial inhibition rate of product which analyzed using the Mann Whitney U test.
Results: Probiotic product inhibited growth of L. monocytogenes by (86 %), Y. pseudotuberculosis (82%), S. aureus (76%), E. coli (74%), S. Typhimurium (73%), P. aeruginosa (69%) and S. epidermidis (67%) respectively. The lowest antagonistic effect was detected against E. faecalis 44%. While probiotic product provided significant growth inhibition on 8 strains (p<0.05), there were not statistically significant growth inhibition for P. vulgaris and E. cloacae (p>0.05).
Conclusion: It was concluded that antagonistic effect of probiotic food supplement on test bacteria. Therefore it may be beneficial to use in bacterial infections.

References

  • 1. Chassaing B, Koren O, Goodrich J K, Poole A C, Srinivasan S, Ley R E, Gewirtz A T. Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome. Nature, 2015; 519(7541), 92.
  • 2. Goulet O. “Potential role of the intestinal microbiota in programming health and disease”, Nutrition Reviews, 2015; 73(suppl_1), 32-40.
  • 3. Roquetto A R, Monteiro N E S, Moura C S, Toreti V C, de Pace F, dos Santos A, ... & Amaya-Farfan J. “Green propolis modulates gut microbiota, reduces endotoxemia and expression of TLR4 pathway in mice fed a high-fat diet”, Food Research International, 2015; 76, 796-803.
  • 4. Huang Y, Shi X, Li Z, Shen Y, Shi X, Wang L, .. & Zhao L. “Possible association of Firmicutes in the gut microbiota of patients with major depressive disorder”, Neuropsychiatric Disease and Treatment, 2018; 14, 3329.
  • 5. Louis P, Hold G L, Flint H J. “The gut microbiota, bacterial metabolites and colorectal cancer”, Nature Reviews Microbiology, 2014;12(10), 661.
  • 6. Carding S, Verbeke K, Vipond D T, Corfe B M, Owen L J. “Dysbiosis of the gut microbiota in disease”, Microbial Ecology in Health and Disease, 2015; 26(1), 26191.
  • 7. Edalati E, Saneei B, Alizadeh M, Hosseini S S, Bialvaei A Z, Taheri K. Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning. New microbes and new infections, 2019; 27, 64-68.
  • 8. de Paula B P, Chávez D W H, Lemos Junior W J F, Guerra A. F, Corrêa M F D, Pereira K S, Coelho, M A Z. Growth parameters and survivability of Saccharomyces boulardii for probiotic alcoholic beverages development. Frontiers in microbiology, 2019 ; 10, 2092.
  • 9. Pais P, Almeida V, Yılmaz M, Teixeira M C. Saccharomyces boulardii: What makes it tick as successful probiotic?. Journal of Fungi, 2020; 6(2), 78.
  • 10. Boynukara B, Gülhan T, Develi Z Ş. Veteriner Hekimlikte Probiyotik Kullanimi. Doğanın Sesi, 2018 ; (1), 43-48.
  • 11. Fijan, S. (2016). Antimicrobial effect of probiotics against common pathogens. In Tech, Venkateswera.
  • 12. Ağyar Yoldaş P , Yoldaş T , Sipahi N . Cytotoxicity of Some Retail Food Supplements. International Journal of Traditional and Complementary Medicine Research. 2020; 1(3): 131-136.
  • 13. Karimi, S., Rashidian, E., Birjandi, M., & Mahmoodnia, L. Antagonistic effect of isolated probiotic bacteria from natural sources against intestinal Escherichia coli pathotypes. Electronic physician, 2018;10(3), 6534.
  • 14. Vaseeharan, B. A. R. P., & Ramasamy, P. Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Letters in applied microbiology, 2003; 36(2), 83-87.
  • 15. Sanders M E, Levy D D. The science and regulations of probiotic food and supplement product labeling. Annals of the New York Academy of Sciences, 2011;1219, E1-E23.
  • 16. AdebayoTayo, B., & Akpeji, S. Probiotic viability, physicochemical and sensory properties of probiotic pineapple juice. Fermentation, 2016; 2(4), 20.
  • 17. de Simone C. The unregulated probiotic market. Clinical Gastroenterology and Hepatology, 2019; 17(5), 809-817.
  • 18. Clinical and Laboratory Standards Institute. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria. The 3rd Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2016.
  • 19. Valdez R M A, ann Ximenez-Fyvie L, Caiaffa K S, Dos Santos V R, Cervantes R M G, Almaguer-Flores A, Duque C. Antagonist effect of probiotic bifidobacteria on biofilms of pathogens associated with periodontal disease. Microbial Pathogenesis, 2021; 150, 104657.
  • 20. Çelik E Ş, Ergün S, Yilmaz S. Bacillus subtilis ve Lactobacillus plantarum Probiyotik Bakterilerinin Bazı Balık Patojenleri Üzerine in vitro Antagonistik Etkisi. Journal of Anatolian Environmental and Animal Sciences, 2019; 4(2), 278-284.
  • 21. Kechagia M, Basoulis D, Konstantopoulou S, Dimitriadi D, Gyftopoulou K, Skarmoutsou N, Fakiri E. M. Health benefits of probiotics: a review. International Scholarly Research Notices, 2013:doi.org/10.5402/2013/481651.
  • 22. Nacakoğlu İ, Sipahi N , Aydın M , Kaya E. Medicinal Plants Meeting with Mud: Phyto-Peloid. International Journal of Traditional and Complementary Medicine Research, 2020; 1(1): 33-41.
  • 23. Kolacek S, Hojsak I, Canani R B, Guarino A, Indrio F, Pot B, ... & Weizman Z. Commercial probiotic products: a call for improved quality control. A position paper by the ESPGHAN Working Group for Probiotics and Prebiotics. Journal of pediatric gastroenterology and nutrition, 2017; 65(1):117-124.
  • 24. Özer M, Özyurt G, Harsa, ŞT.. Probiyotik ve prebiyotiklerin bağırsak-beyin aksına etkisi. Akademik Gıda, 2019;17(2):269-280.
  • 25. Mollakhalili MN, Mortazavian A M, Sohrabvandi S, Cruz AGD & Mohammadi R. Probiotic supplements and food products: comparison for different targets. Applied Food Biotechnology, 2017;4(3): 123-132.
  • 26. Wang Y, Jiang Y, Deng Y, Yi C, Wang Y, Ding M, ... & Wong A. Probiotic Supplements: Hope or Hype?. Frontiers in Microbiology, 2020;11, 160.
  • 27. Iglesias M B, Abadias M, Anguera M, Sabata J, & Vi I. Antagonistic effect of probiotic bacteria against foodborne pathogens on fresh-cut pear. LWT-Food Science and Technology, 2017; 81, 243-249.
  • 28. Corr S C, Gahan C G, & Hill C. Impact of selected Lactobacillus and Bifidobacterium species on Listeria monocytogenes infection and the mucosal immune response. FEMS Immunology & Medical Microbiology, 2007; 50(3), 380-388.
  • 29. Maragkoudakis P A, Zoumpopoulou G, Miaris C, Kalantzopoulos G, Pot B, Tsakalidou E. Probiotic potential of Lactobacillus strains isolated from dairy products. International Dairy Journal, 2006; 16(3), 189-199.
  • 30. Guo S, Li L, Xu B, Li M, Zeng Q, Xiao H, ... & Zhang, G. A simple and novel fecal biomarker for colorectal cancer: ratio of Fusobacterium nucleatum to probiotics populations, based on their antagonistic effect. Clinical chemistry, 2018; 64(9), 1327-1337.
  • 31. Gudisa A. In vitro antagonistic effect of probiotic LAB isolated from traditional fermented products against some antibiotics resistant food borne pathogens 2021. (Doctoral dissertation).
  • 32. O’Connor P M, Kuniyoshi T M, Oliveira R P, Hill C, Ross R P, & Cotter P. D. Antimicrobials for food and feed; a bacteriocin perspective. Current opinion in biotechnology, 2020; 61, 160-167.
  • 33. Yu H S, Lee N K, Choi A J, Choe J S, Bae C H, Paik H D. Antagonistic and antioxidant effect of probiotic Weissella cibaria JW15. Food science and biotechnology, 2019; 28(3), 851-855.
There are 33 citations in total.

Details

Primary Language English
Subjects Traditional, Complementary and Integrative Medicine
Journal Section Research Articles
Authors

Nisa Sipahi 0000-0001-8915-3545

Publication Date August 16, 2021
Submission Date March 8, 2021
Published in Issue Year 2021 Volume: 02 Issue: 02

Cite

APA Sipahi, N. (2021). Investigation of Antagonistic Effect of Probiotic Food Supplement against Different Strains of Bacteria. International Journal of Traditional and Complementary Medicine Research, 02(02), 95-100. https://doi.org/10.53811/ijtcmr.893132

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