Application of Experimental Design Method in Bioprocess Studies

Yıl 2020, Cilt: 1 Sayı: 2, 188 - 197, 31.12.2020

Meryem Sena Akkuş Sema Karabudak

Öz

Experimental design methods are the techniques that reduce the costs of the experiments performed, increase the reliability of the results, and reach the desired goal in a short time with less experimentation. In the work of biological processes, there are many factors aimed at the desired goal, that affect the target directly or indirectly. It is possible to reach the most accurate and accurate result with the appropriate experimental design method. In this study, information is given about how the full factorial design, Taguchi method, Box-Behnken design, central composite design, and Plackett-Burman design methods, which are among the widely used experimental design methods in the literature, reach the most appropriate value conditions in implementations and application examples of these methods in bioprocess studies.

Anahtar Kelimeler

Experimental design methods, Process optimization, Bioprocess

Biyoproses Çalışmalarında Deneysel Tasarım Yönteminin Uygulanması

Öz

Deney tasarımı yöntemleri yapılan deneylerin maliyetlerini düşüren, sonuçların güvenilirliğini artıran, az deneyle istenen hedefe kısa sürede ulaştıran tekniklerdir. Biyolojik proseslerin çalışmalarında istenen hedefe yönelik, hedefi doğrudan veya dolaylı olarak etkileyen birçok faktör bulunmaktadır. Uygun deney tasarım yöntemiyle gerçeğe en yakın ve hassas sonuca ulaşmak mümkündür. Yapılan bu çalışmada literatürde yaygın olarak kullanılan deney tasarım yöntemlerinden tam faktöriyel dizaynı, Taguchi metodu, Box–Behnken tasarımı, merkezi kompozit tasarımı ve Plackett–Burman tasarım yöntemlerinin uygulamalarda en uygun değer koşullarına nasıl ulaştığını ve biyoproses çalışmalarında bu yöntemlerin uygulama örnekleri hakkında bilgi verilmiştir.

Anahtar Kelimeler

Deneysel tasarım yöntemleri, Proses optimizasyonu, biyoproses

Kaynakça

• Alshehria, A.N., Ghanem, K.M., Al-Garni, S.M. 2016. Application of a five level central composite design to optimize operating conditions for electricity generation in a microbial fuel cell, Journal of Taibah University for Science, 10(6), 797-804.
• Arslan, A., Topkaya, E., Bingöl, D., Veli, S. 2018. Removal of anionic surfactant sodium dodecyl sulfate from aqueous solutions by O3/UV/H2O2 advanced oxidation process: Process optimization with response surface methodology approach, Sustainable Environment Research, 28(2), 65-71.
• Coban, I., Sargin, S., Celiktas, M.S., Yesil-Celiktas, O. 2012. Bioethanol production from raffinate phase of supercritical CO2 extracted Stevia rebaudiana leaves, Bioresource technology, 120, 52-59.
• Demir, Ö., Aksu, B., Özsoy, Y. 2017. İlaç formülasyonu geliştirilmesinde deney tasarımı (doe) seçimi ve kullanımı.
• El Enshasy, H.A., Elsayed, E.A., Suhaimi, N., Abd Malek, R., Esawy, M. 2018. Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system, BMC biotechnology, 18(1), 71.
• Fontes, G.C., Fonseca Amaral, P.F., Nele, M., Zarur Coelho, M.A. 2010. Factorial design to optimize biosurfactant production by Yarrowia lipolytica, Journal of Biomedicine and Biotechnology, 2010.
• Geiger, E.O. (2014). Statistical methods for fermentation optimization Fermentation and Biochemical Engineering Handbook (pp. 415-422): Elsevier.
• Ghanem, K.M., Al-Garni, S.M., Al-Shehri, A.N. 2009. Statistical optimization of cultural conditions by response surface methodology for phenol degradation by a novel Aspergillus flavus isolate, African Journal of Biotechnology, 8(15).
• İç, Y.T., Duran, H., Keçeci, B., Emrecan, İ., Bilgiç, B. 2016. Çok yanıtlı Taguchi eniyilemesine yönelik bir bilgisayar uygulamasının geliştirilmesi, Politeknik Dergisi, 19(3), 311-323.
• Jacyna, J., Kordalewska, M., Markuszewski, M.J. 2019. Design of Experiments in metabolomics-related studies: An overview, Journal of pharmaceutical and biomedical analysis, 164, 598-606.
• Kammoun, R., Naili, B., Bejar, S. 2008. Application of a statistical design to the optimization of parameters and culture medium for α-amylase production by Aspergillus oryzae CBS 819.72 grown on gruel (wheat grinding by-product), Bioresource technology, 99(13), 5602-5609.
• Karslıoğlu, Z., Baray, A., Özçkar, N. 2015. PVC tesisat bağlanti parçalari üretiminde fire probleminin çözümünde Plackett-Burman deney tasarimi yaklaşımı, İstanbul Üniversitesi İşletme Fakültesi İşletme İktisadı Enstitüsü Yönetim Dergisi, 26(79), 180-207.
• Keskin Gündoğdu, T., Deniz, I., Çalışkan, G., Şahin, E.S., Azbar, N. 2016. Experimental design methods for bioengineering applications, Critical reviews in biotechnology, 36(2), 368-388.
• Lee, J.-E., Vadlani, P.V., Faubion, J. 2017. Corn bran bioprocessing: Development of an integrated process for microbial lipids production, Bioresource Technology, 243, 196-203. doi:https://doi.org/10.1016/j.biortech.2017.06.065
• Lee, K.-M., Gilmore, D.F. 2006. Statistical experimental design for bioprocess modeling and optimization analysis, Applied biochemistry and biotechnology, 135(2), 101-115.
• Mohapatra, P.D., Maity, C., Rao, R., Pati, B., Mondal, K. 2009. Tannase production by Bacillus licheniformis KBR6: Optimization of submerged culture conditions by Taguchi DOE methodology, Food Research International, 42(4), 430-435.
• Muthuvelayudham, R., Viruthagiri, T. 2010. Application of central composite design based response surface methodology in parameter optimization and on cellulase production using agricultural waste, International Journal of Chemical and Biological Engineering, 3(2), 97-104.
• Özkan, G., Akkuş, M.S., Özkan, G. 2019. The effects of operating conditions on hydrogen production from sodium borohydride using Box-Wilson optimization technique, International Journal of Hydrogen Energy, 44(20), 9811-9816. doi:https://doi.org/10.1016/j.ijhydene.2018.12.134
• Plackett, R.L., Burman, J.P. 1946. The design of optimum multifactorial experiments, Biometrika, 33(4), 305-325.
• Prakash, G., Srivastava, A.K. 2005. Statistical media optimization for cell growth and azadirachtin production in Azadirachta indica (A. Juss) suspension cultures, Process Biochemistry, 40(12), 3795-3800. Savaşkan, M., Taptık, Y., Ürgen, M. 2010. Deney tasarımı yöntemi ile matkap uçlarında performans optimizasyonu, İTÜ Dergisi, 3(6), 117-128.
• Singh, A., Bishnoi, N.R. 2012. Enzymatic hydrolysis optimization of microwave alkali pretreated wheat straw and ethanol production by yeast, Bioresource Technology, 108, 94-101.
• Singh, R. 2015. Chapter 3-Hybrid Membrane Systems–Applications and Case Studies, Membrane Technology and Engineering for Water Purification (Second Edition), Publisher: Elsevier Science & Technology Books. Pages 179, 281.
• Tari, C., Ustok, F.I., Harsa, S. 2010. Production of food grade β-galactosidase from artisanal yogurt strains, Food Biotechnology, 24(1), 78-94.
• Türk, T. 2016. Merkezi Kompozit Tasarımı Kullanılarak Bakır Cürufundan Arsenik Liçinin İncelenmesi, Yerbilimleri Dergisi, 37(3), 193-202.
• Verma, A.S., Agrahari, S., Rastogi, S., Singh, A. 2011. Biotechnology in the realm of history, Journal of Pharmacy and Bioallied Sciences, 3(3), 321.
• Vijayaraghavan, P., Prakash Vincent, S.G., Dhillon, G.S. 2016. Solid-substrate bioprocessing of cow dung for the production of carboxymethyl cellulase by Bacillus halodurans IND18, Waste Management, 48, 513-520. doi:https://doi.org/10.1016/j.wasman.2015.10.004
• Wang, J., Wan, W. 2009. Experimental design methods for fermentative hydrogen production: a review, International journal of hydrogen energy, 34(1), 235-244.
• Wang, K.-S., Chen, J.-H., Huang, Y.-H., Huang, S.-L. 2013. Integrated Taguchi method and response surface methodology to confirm hydrogen production by anaerobic fermentation of cow manure, International journal of hydrogen energy, 38(1), 45-53.
• Wasli, A.S., Salleh, M.M., Abd-Aziz, S., Hassan, O., Mahadi, N.M. 2009. Medium optimization for chitinase production from Trichoderma virens using central composite design, Biotechnology and Bioprocess Engineering, 14(6), 781-787.
• Yang, W.p., Tarng, Y. 1998. Design optimization of cutting parameters for turning operations based on the Taguchi method, Journal of materials processing technology, 84(1-3), 122-129.
• Yatsyshyn, V.Y., Fedorovych, D.V., Sibirny, A.А. 2010. Medium optimization for production of flavin mononucleotide by the recombinant strain of the yeast Candida famata using statistical designs, Biochemical Engineering Journal, 49(1), 52-60.
• Zümrüt, Z. (2009). Tam Faktöriyel Deney Tasarımı Tekniği İle Hidroksiapatit Kaplı Titanyum İmplant Malzemelerin Mekanik Özellikleri Üzerine Parametrelerin Etkisi. Fen Bilimleri Enstitüsü.