Fenarimol'e Maruz Kalan Sıçanların Çeşitli Dokularında LDH Aktivitesindeki Değişiklikler

Yıl 2025, Cilt: 6 Sayı: 1, 73 - 85, 30.06.2025

Egemen Dere

Öz

Fenarimol, özellikle üzüm bağlarında mantar hastalıklarıyla mücadelede kullanılan, meyve ve sebze yetiştiriciliğinde yaygın şekilde tercih edilen bir fungisittir. Hem östrojenik hem de antiandrojenik aktivite gösteren bu bileşik, gama-glutamiltransferaz, glikoz-6-fosfataz ve alkali fosfataz gibi önemli enzimler üzerinde etkili olabilmektedir. Bu çalışmada ise laktat dehidrogenaz (LDH) enzimi ele alınmıştır.
Fenarimol, LD₅₀ dozu olan 200mg kg-1 düzeyinde, erkek ve dişi sıçanlara intraperitoneal olarak uygulanmıştır. Uygulamayı takiben 2, 4, 8, 16, 32, 64. ve 72. saatlerde sıçanların karaciğer, böbrek, beyin ve ince bağırsak dokularından alınan örneklerde LDH enzim aktivitesindeki değişimler belirlenmiştir.
Elde edilen bulgular, Fenarimol uygulamasının incelenen tüm dokularda LDH aktivitesini artırdığını göstermiştir. Bu artış, her iki cinsiyette de 72. saate doğru azalma eğilimi göstermiştir. LDH aktivitesindeki bu artışların, ilgili dokularda hücresel hasar meydana geldiğine işaret ettiği düşünülmektedir.

Anahtar Kelimeler

fenarimol , karaciğer , böbrek , beyin , ince bağırsak

Proje Numarası

F(U)-2003/65

The Changes in LDH Activity in Various Tissues of Rats Exposed to Fenarimol

Öz

Fenarimol is a fungicide widely used in fruit and vegetable cultivation, particularly in vineyards, to combat fungal diseases. Exhibiting both estrogenic and antiandrogenic activities, this compound also influences several key enzymes, including gamma-glutamyltransferase, glucose-6-phosphatase, and alkaline phosphatase. In the present study, the enzyme lactate dehydrogenase (LDH) was selected as a biochemical marker to evaluate tissue-specific effects of Fenarimol exposure.
Fenarimol was administered intraperitoneally to both male and female rats at its LD₅₀ dose (200 mg/kg). Following administration, changes in LDH enzyme activity were assessed in liver, kidney, brain, and small intestine tissues at various time intervals: 2, 4, 8, 16, 32, 64, and 72 hours.
The results indicated a significant increase in LDH activity in all examined tissues post-exposure. However, this elevated enzyme activity gradually declined toward the 72-hour mark in both sexes. These increases in LDH activity suggest that Fenarimol induces notable tissue damage, reflecting its toxicological impact on vital organs.

Anahtar Kelimeler

fenarimol , liver , kidney , brain , small intestine

Etik Beyan

Bu çalışmada kullanılan tüm hayvan deneyleri, Bursa Uludağ Üniversitesi Hayvan Deneyleri Etik Kurulu’nun onayı ile gerçekleştirilmiştir (3. 06. 2003/3]. Deneyler sırasında hayvan refahı, 6343 sayılı Veteriner Hizmetleri, Bitki Sağlığı, Gıda ve Yem Kanunu ve ilgili yönetmeliklere uygun olarak sağlanmıştır. Tüm prosedürler, hayvanların gereksiz yere acı çekmesini önleyecek şekilde planlanmış ve uygulanmıştır.

Destekleyen Kurum

Bu çalışma Bursa Uludağ Üniversitesi Bilimsel Araştırma Fonu tarafından desteklenmiştir. F(U)-2003/65

Proje Numarası

F(U)-2003/65

Teşekkür

Çalışmaya katkılarından dolayı Sayın Ferda Arı ve Hakan Tosunoğlu'na teşekkür ederim

Kaynakça

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