Effect Of Piroxicam On Ileal Anastomoses In Wistar Albino Rats

Öz

Objective: The purpose of our study was to examine the influence of piroxicam, non-steroid anti-inflammatory drug, on healing of ileal anastomoses in rats.

Materials and Methods: 20 rats were divided randomly into two groups and submitted to resection and ileal anastomosis. The test group received piroxicam, 2mg/kg/day, beginning one day before operation until sacrification. The animals were sacrificed on third and seventh days after the operation. Tissue homogenate, indicator of tissue collagen content, was hydrolyzed with HQ
and then evaporated to dryness. Evaporated sample was deproteinized in HC1 and after this processes hydroxyprolin was measured spectrophotometrically in supernatant fraction in which taken isopropanol.

Results: In test group, hydroxyproline concentrations were slightly reduced on 3r d day but again increased to the preoperational levels on 7th day after anastomosis (p> 0.05). On the other hand, in the control group, ileal hydroxyproline concentrations were reduced significantly on 3rd day but increased to preoperational levels on 7t h day after the operation.

Conclusion: In conclusion, piroxicam might improve hydroxyproline levels during initial phase of wound healing. 

Anahtar Kelimeler

• Piroxicam
• Anastomoses
• Wistar Albino Rats

Wistar Albino Sıçanlarda Ileal Anastomoz iyileşmesinde Piroksikamın Etkisi

Öz

Amaç: Çalışmamızın amacı bir non steroid antiinflamatuar ilaç olan piroksikamın sıçanlarda ileal anastomoz iyileşmesindeki etkisini incelemekti.

Gereç ve Yöntem: 20 sıçan randomize olarak iki gruba ayrılarak rezeksiyonlan ve ileal anastomozlan yapıldı. Deney grubuna operasyondan bir gün önceden sakrifiye edilene kadar 2mg/kg/gün piroksikam verildi. Hayvanlar operasyondan sonraki üçüncü ve yedinci günlerde sakrifiye edildi. Dokunun kollajen miktarını göstergesi olarak doku homojenatı HC1 içinde hidroliz edilmesine takiben evapore edildi. Evapore edilmiş örnek HC1 içinde deproteinize edilerek işlem sonrasında süpernatan fraksiyonu isopropanol içine alınarak hidroksiprolin spektrofotometrik olarak tayin edildi.

Bulgular: Deney grubunda hidroksiprolin konsantrasyonları üçüncü günde hafif düşerken anastomoz sonrası yedinci günde operasyon öncesi seviyeye yükseldi. Diğer yandan kontrol grubunda ileal hidroksiprolin konsantrasyonu üçüncü günde anlamlı olarak azalırken operasyon sonrasındaki yedinci günde operasyon öncesi seviyeye yükseldi.

Tartışma: Sonuç olarak piroksikam yara iyileşmesinin başlangıç fazı süresince hidroksiprolin düzeylerini arttırabilmektedir

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